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  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
    • Overview
    • Protocols
    • Applications
    • Technical notes
    • Stem cell posters
    • Webinars
    • Videos
    • Stem cell FAQs
    • Citations
    • Stem cell selection guides
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Real-time PCR
  • cDNA synthesis
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Home › Learning centers › Stem cell research

Learning centers

  • Automation systems
    • SmartChip Real-Time PCR System introduction
      • High-throughput detection of SARS-CoV-2
      • SmartChip Real-Time PCR System overview
      • SmartChip Real-Time PCR System technical specifications
      • SmartChip Real-Time PCR System applications
        • Antibiotic resistance genes
          • Screening for antibiotic resistance genes in manure and sewage
          • Uncovering antibiotic resistance genes in soil, sediment, and sludge
          • Tracking down antibiotic resistance genes in hospitals
          • Identifying antibiotic resistance genes in water
        • mRNA, miRNA, and lncRNA as disease biomarkers
        • Pathogen detection in human samples and food
        • Genotyping using animal and blood samples
      • SmartChip Real-Time PCR System video resources
        • Webinar: detection of ARGs and SARS-CoV-2 in wastewater
        • Webinar: antibiotic resistance screening
        • SmartChip tutorials
    • ICELL8 introduction
      • ICELL8 technology overview
      • ICELL8 cx technical specifications
      • ICELL8 technical specifications (original system)
      • ICELL8 system vs plate-seq
      • Webinars
      • Technical notes
        • Enhancing biomarker discovery with SMART-Seq Pro kit and ICELL8 cx system
        • ICELL8 cx system target enrichment for fusions
        • ICELL8 cx system reagent formulation and dispense guidelines
        • Improved detection of gene fusions, SNPs, and alternative splicing
        • Full-length transcriptome analysis
        • High-throughput single-cell ATAC-seq
        • Protocol: High-throughput single-cell ATAC-Seq
        • Single-cell identification with CellSelect Software
        • Single-cell analysis elucidates cardiomyocyte differentiation from iPSCs
        • Combined TCR profiling and 5’ DE in single cells
        • Automated, high-throughput TCR profiling
      • Sample preparation protocols
        • Basic cell preparation for the ICELL8 cx system
        • Protocol: Nuclei isolation from mammalian cells
        • Protocol: Mouse cardiomyocyte preparation
        • Isolate cells of any size
      • Video resources
      • Citations
      • Posters
      • System & software notices
    • Apollo library prep system introduction
      • Automated VeriSeq library preparation for PGS
      • Apollo library prep system citations
      • SMART-Seq v4 chemistry for the Apollo system
      • Apollo library prep system overview
      • Apollo system technical specifications
      • In-tip bead separation on the Apollo system
  • Next-generation sequencing
    • Product line overview
    • Technical notes
      • DNA-seq
        • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
        • Next-gen WGA method for CNV and SNV detection from single cells
        • Low-volume DNA shearing for ThruPLEX library prep
        • Low-input whole-exome sequencing
        • Cell-free nucleic acid sequencing
        • DNA-seq from FFPE samples
        • ThruPLEX HV data sheet
        • Improvements to ThruPLEX HV
        • ThruPLEX HV outperforms NEBNext Ultra II
        • Comparing ThruPLEX HV PLUS to Kapa and NEBNext
        • Low cell number ChIP-seq using ThruPLEX DNA-Seq
        • Accurate detection of low-frequency variants using molecular tags
      • Immune Profiling
        • TCRv2 kit validated for rhesus macaque samples
        • TCR repertoire profiling from mouse samples (bulk)
        • BCR repertoire profiling from mouse samples (bulk)
        • Improved TCR repertoire profiling from human samples (bulk)
        • TCR repertoire profiling from human samples (single cells)
        • BCR repertoire profiling from human samples (bulk)
      • RNA-seq
        • All-in-one cDNA synthesis and library prep from single cells
        • Automation-friendly, all-in-one cDNA synthesis and library prep
        • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
        • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
        • Full-length mRNA-seq for target capture
        • Stranded libraries from single cells
        • Stranded libraries from picogram-input total RNA (v3)
        • Stranded libraries from 100 pg-100 ng total RNA
        • Stranded libraries from 100 ng - 1 ug total RNA
        • Stranded libraries from FFPE inputs (v2)
        • Nonstranded libraries from FFPE inputs
      • Epigenetic sequencing
        • ChIP-seq libraries for transcription factor analysis
        • ChIP-seq libraries from ssDNA
        • Full-length small RNA libraries
        • Methylated DNA-seq with MBD2
      • Reproductive health technologies
        • Embgenix PGT-A (CE-IVD & RUO)
    • Featured kits
    • Technology and application overviews
      • SMART technology
      • Single-cell mRNA-seq
      • Total RNA-seq
      • Biomarker discovery with RNA-seq
      • SMART-Seq PLUS solutions
      • Whole genome amplification from single cells
      • Sequencing depth for ThruPLEX Tag-seq
    • FAQs and tips
      • RNA-seq FAQs
      • RNA-seq tips
      • Positive and negative controls in scRNA-seq
      • DNA-seq FAQs
      • ChIP-seq FAQs
      • Indexing FAQs
      • TCR-seq methods: Q&A
      • STORM-seq Q&A
      • Pushing the limits Q&A
      • Liver metabolic function Q&A
      • Neural multiomics Q&A
    • DNA-seq protocols
      • Using UMIs with ThruPLEX Tag-Seq HV
      • Exome capture with Illumina Nextera Rapid Capture
      • Targeted capture with Roche NimbleGen SeqCap EZ
      • Targeted capture with IDT xGen panels
      • Targeted capture with Agilent SureSelectXT
      • Targeted capture with Agilent SureSelectXT2
      • Targeted capture with Agilent SureSelectQXT
      • Plasma preparation for ThruPLEX Plasma-Seq workflow
    • Bioinformatics resources
      • Cogent NGS Analysis Pipeline
        • Cogent NGS Analysis Pipeline notices
      • Cogent NGS Discovery Software
        • Cogent NGS Discovery Software notices
      • Cogent NGS Immune Profiler
        • Cogent NGS Immune Profiler Software notices
        • Cogent NGS Immune Profiler Software videos
      • Cogent NGS Immune Viewer
      • Embgenix Analysis Software
      • ICELL8 scTCR Analyzer
      • SMARTer Human scTCR Demultiplexer
      • SMART-Seq DE3 Demultiplexer
    • Newsletters
      • 2021 H1 NGS newsletter
      • 2020 NGS newsletter
    • Webinars
      • Harnessing the power of full-length transcriptome analysis for biomarker discoveries
      • SMART-Seq Pro kits for biomarker detection
      • Takara Bio Single-Cell Workshop, Spring 2021
      • Capturing biological complexity by high-resolution single-cell genomics
      • Sensitivity and scale for neuron multiomics
      • Taking single-cell RNA-seq by STORM
      • Pushing the limits of sensitivity for single-cell applications
      • Liver metabolic function, dissecting one cell at a time
      • Single-Cell Workshop at 2020 NextGen Omics Series UK
      • TCR-seq methods: when to use which
      • Immunogenomics to accelerate immunotherapy
      • Total RNA sequencing of liquid biopsies
      • MeD-Seq, a novel method to detect DNA methylation
      • Automating full-length single-cell RNA-seq libraries
      • Single-cell DNA-seq
      • Single-cell whole transcriptome analysis
    • Citations
      • Epigenetics citations
      • Microbiome citations
      • PicoPLEX citations
      • SMARTer RNA-seq citations
      • ThruPLEX DNA-seq citations
      • ThruPLEX Plasma-seq citations
    • Posters
  • Gene function
    • Gene editing
      • Gene editing product finder
      • Gene editing tools and information
        • sgRNA design tools
        • Tools for successful CRISPR/Cas9 genome editing
        • Gene editing posters
        • Customer data for Guide-it products
        • How to design sgRNA sequences
        • Introduction to the CRISPR/Cas9 system
        • Gene editing of CD3+ T cells and CD34+ HSCs
      • CRISPR/Cas9 knockouts
        • Mutation detection kit comparison
        • Screening for effective guide RNAs
        • Monoallelic versus biallelic mutants
        • Indel identification kit for mutation characterization
      • CRISPR/Cas9 knockins
        • Choosing an HDR template format
        • Homology-directed repair FAQs
        • Mouse CRISPR knockin protocol
        • Site-specific gene knockins using long ssDNA
        • Efficient CRISPR/Cas9-mediated knockins in iPS cells
        • Oligo design tool for detecting precise insertions
          • Oligo design tool user guide (insertions)
      • Genome-wide screening
        • CRISPR library screening
        • CRISPR library screening webinar
        • Phenotypic screen using sgRNA library system
      • Creating and screening for SNPs
        • SNP detection with knockin screening kit
        • Oligo design tool for SNP screening
          • Oligo design tool user guide (SNPs)
        • Sign up: SNP engineering webinar
        • Guide-it SNP Screening Kit FAQs
      • CRISPR/Cas9 delivery methods
        • Electroporation-grade Cas9 for editing in diverse cell types
        • CRISPR/Cas9 gene editing with AAV
        • CRISPR/Cas9 gesicles overview
        • Cas9 Gesicles—reduced off-target effects
        • sgRNA-Cas9 delivery to many cell types
        • Tet-inducible Cas9 for gene editing
      • Cre recombinase
        • Control your Cre recombinase experiments
        • Fast Cre delivery with gesicle technology
    • Viral transduction
      • Recombinant virus comparison
      • Product finder
      • Transduction posters
      • Lentivirus
        • Customizable SARS-CoV-2 pseudovirus
        • Lenti-X FAQs
        • Lentiviral workflow
        • Lentiviral products guide
        • Lentivirus biosafety
        • Lentiviral tips
        • Lentiviral vectors
        • Lenti-X packaging
        • High-throughput lentivirus production
        • Lenti-X Concentrator
        • Lentiviral titration
        • Lenti-X GoStix Plus video protocols
        • Lenti-X GoStix Plus FAQs
        • Rapid lentivirus titration by p24 ELISA
        • Lentiviral particles
        • Lentiviral particles—fluorescent
        • Lentiviral particles webinar
        • Lentiviral particles FAQs
      • Retrovirus
        • Retroviral products
        • Retro-X FAQs
        • Retro-X packaging
        • Retro-X Concentrator
      • Adeno-associated virus
        • AAV workflow
        • AAV products
        • AAV FAQs
        • AAV tech notes
          • Customer data: Purified AAV9 delivery (mouse brain)
          • Customer data: Purified AAV9 delivery (songbird brain)
          • Serotype-independent AAV vector purification
          • Customer data: Purified AAV2 delivery (mouse brain)
        • AAV videos
          • AAV2 purification video
          • AAVpro "All Serotypes" protocols
        • AAVpro Concentrator overview
      • Adenovirus
        • Adenoviral FAQs
        • Adenoviral products
        • Fastest, easiest adenoviral system ever
        • Tet-inducible adenovirus
        • Adenovirus purification kits
        • Adenovirus purification mega-scale
        • Adenovirus rapid titer
        • Adenoviral titration
    • T-cell transduction and culture
      • Technology overview
      • Adoptive T-cell therapy (ACT)
      • RetroNectin FAQs
      • Hematopoietic cell transduction
      • T-cell expansion
      • Serum-free T-cell culture
      • CultiLife culture bags protocol
      • Cytokine analysis
    • Inducible systems
      • iDimerize systems
        • Inducible protein-protein interactions—iDimerize systems
        • iDimerize systems journal club
        • iDimerize in vivo protocol
        • iDimerize systems citations
        • ARGENT cell signaling regulation kits from ARIAD
      • ProteoTuner systems
        • ProteoTuner technology overview
        • ProteoTuner citations
        • Inducible protein stabilization systems product selection guide
      • Tet-inducible systems
        • Tet systems product selection guide
        • Tet systems overview
        • Tet-One technology overview
        • Tet-On 3G plasmid system kit components
        • Tet-On 3G lentiviral system kit components
        • Tet system webinars
    • Transfection reagents
      • Xfect Transfection Reagent
      • Transfection tips
      • Cell lines transfected with the Xfect reagent
      • Protein transfection FAQs
      • Xfect RNA Transfection Reagent
      • Transfection reagent products
      • Protocol: transfection of cerebellar slice cultures with Xfect reagent
      • Calcium phosphate transfection of neurons
    • Fluorescent proteins
      • Fluorescent protein vector finder
      • Fluorescent protein excitation and emission maxima
      • Fluorescent protein antibodies selection guide
      • Fluorescent protein antibody citations
        • GFP antibody citations
        • RFP antibody citations
      • Fluorescent protein quick guide
      • Fluorescent retroviral expression vectors
      • Verify miRNA expression
      • Find EGFP vector alternatives
    • Cell biology assays
      • Technical notes
        • Cell viability and proliferation measurement
      • Citations
  • Stem cell research
    • Overview
      • Stem cell research products and services
      • Stem cell media products
      • Cardiomyocyte products
      • Hepatocyte products and services
      • iPS cell to hepatocyte differentiation overview
      • Cellartis human pluripotent stem cell services
    • Protocols
      • Hepatocytes
        • Video protocols for hiPS-HEP v2 cells
        • Getting started with hepatocyte differentiation
      • Pluripotent stem cells
        • Transferring iPSCs on MEFs to DEF-CS
        • Transferring iPSCs from other media to DEF-CS
        • Spin embryoid body formation
        • Reprogramming PBMCs
        • Reprogramming fibroblasts
      • Cardiomyocytes
        • Cardiomyocytes in FLIPR 384-well plate format
        • Cardiomyocytes on the Patchliner system
        • Cardiomyocytes on the Maestro MEA system
        • Cardiomyocytes on the MED64 MEA system
        • Cardiomyocytes on the CardioExcyte 96 system
        • Cardiomyocytes on the xCELLigence RTCA CardioECR system
    • Applications
    • Technical notes
      • Pluripotent stem cells
        • Using the DEF-CS system to culture human iPS cells
        • Comparison of the Cellartis DEF-CS system with other vendors' human iPS cell culture systems
        • Reprogramming PBMCs
        • Reprogramming fibroblasts
      • Gene editing in hiPS cells
        • Tagging an endogenous gene with AcGFP1 in hiPS cells
        • Tagging an endogenous gene with a myc tag in hiPS cells
        • Generating clonal hiPS cell lines deficient in CD81
        • Introducing a tyrosinemia-related SNP in hiPS cells
        • Inserting an expression cassette into the AAVS1 locus in hiPS cells
        • Editing hiPS cells using electroporation
        • Editing hiPS cells using gesicle technology
        • Single-cell cloning of hiPS cells
      • Organoids
        • Liver organoid differentiation from iPSCs for prediction of drug-induced liver injury
        • Generation of embryonic organoids using NDiff 227 neural differentiation medium
      • Beta cells
        • Beta cells for disease modeling
      • Hepatocytes
        • hiPS-HEP cells for disease modeling
        • hiPS-HEP cells for drug metabolism studies
        • Power medium for long-term human primary hepatocyte culture
        • iPS cell to hepatocyte differentiation system
      • Cardiomyocytes
        • Making engineered heart tissue with cardiomyocytes
      • Neural stem cells
        • RHB-A neural stem cell medium
    • Posters
    • Webinars
      • Using hiPS gene editing to create a tyrosinemia disease model
    • Videos
      • Hepatocyte offerings
    • FAQs
      • Cellartis DEF-CS 500 Culture System FAQs
      • Cellartis enhanced hiPS-HEP FAQs
      • Cellartis iPS Cell to Hepatocyte Differentiation System FAQs
    • Citations
      • Cellartis MSC Xeno-Free Culture Medium
      • Cellartis Power Primary HEP Medium
      • Cellartis DEF-CS 500 Culture System
      • Cellartis Enhanced hiPS-HEP cells
      • Cellartis hES-MP 002.5
      • Cellartis hPS cell-derived cardiomyocytes
      • Cellartis iPS Cell to Hepatocyte Differentiation System
      • GS1-R
      • 2i mES/iPSC medium
      • iMatrix-511
      • 3i mES/iPSC medium
      • NDiff 227
      • NDiff N2
      • RHB-A
      • STEM101
      • STEM121
      • STEM123
    • Selection guides
      • Stem cell antibody selection guide
      • Stem cell media product finder
      • Stem cell tools product finder
      • Hepatocyte product finder
  • Protein research
    • Capturem technology
      • Capturem protocols
      • Capturem tech notes and applications
      • FAQs about Capturem technology
      • Capturem technology citations
      • Capturem posters
    • Antibody immunoprecipitation
      • IP and Co-IP of cardiac voltage-gated ion channel proteins
      • Tech note: thiophilic antibody purification resins
      • Tech note: IP and Co-IP
    • His-tag purification
      • Purification methods overview
      • TALON resin selection guide
      • Selection guide: His60 resin
      • xTractor Buffer is optimized for superior protein yield
      • Why tag a protein?
      • Tech note: cobalt resin
      • Simplified purification of active, secreted his-tagged proteins
      • Overview: His60
      • Tech note: Capturem technology
      • Tech note: Capturem large volume
      • Magnetic beads
      • FAQs: TALON
      • Protocols
        • Video: Capturem his maxiprep
        • Video: Capturem his miniprep
        • Visual protocol: Capturem his maxiprep
        • Visual protocol: Capturem his miniprep
        • Capturem nickel column reagent compatibility
        • TALON reagent compatibility
        • His60 reagent compatibility
        • TALON: Native vs denaturing purification
        • Protocol: denaturing purification with TALON resin, imidazole elution
        • Protocol: native purification with TALON resin, imidazole elution
        • Protocol: native purification with TALON resin, pH elution
    • Other tag purification
      • Streptavidin-based enrichment using Capturem technology
      • Selection guide: peptide tags
      • Myc-tagged protein purification overview
      • GST-tagged protein purification overview
    • Phosphoprotein and glycoprotein purification
      • Non-tagged protein purification overview
      • Phosphoprotein purification overview
    • Matchmaker Gold yeast two-hybrid systems
      • Matchmaker Gold Yeast Two-Hybrid System
      • Make your own library for yeast two-hybrid screening
      • Mate and Plate yeast two-hybrid cDNA libraries
      • Aureobasidin A for improved selectable drug resistance in yeast
    • Expression systems
      • Protein expression overview
      • Insect expression overview
      • Mammalian expression overview
      • pHEK293 Ultra expression overview
      • OKT3 expression in mammalian cells
      • Bacterial expression overview
  • PCR
    • Citations
      • PrimeSTAR HS
      • EmeraldAmp MAX
      • Terra PCR Direct
      • EmeraldAmp GT
      • Takara Ex Taq
      • PrimeSTAR Max
      • PrimeSTAR GXL
      • Takara LA Taq
      • SpeedSTAR HS
      • Takara Taq and Taq HS
      • Titanium Taq
    • Selection guides
      • PCR selection guide
      • Direct PCR selection guide
      • Fast PCR selection guide
    • PCR enzyme brochure
    • Technical notes
      • Rapid, high-performance multiplex PCR
      • EmeraldAmp outperforms MyTaq Red mix
      • Fast and accurate PCR
      • Methylation studies
      • Hot-start PCR
      • Long-range PCR with LA Taq
      • Direct PCR from human nail
      • Direct PCR from meat samples
      • Megaprimer PCR with PrimeSTAR GXL
      • Amplifying GC-rich templates
      • Titanium Taq for high-throughput genotyping
      • Colony PCR in under an hour
      • High-throughput endpoint PCR
      • Direct PCR from blood
      • PrimeSTAR GXL for targeted sequencing
      • Detecting somatic mosaicism using massively parallel sequencing
    • FAQ
      • Primer design
      • Optimization
      • Troubleshooting
      • Applications and conditions
      • Shipping, storage, and handling
    • Go green with lyophilized enzymes
  • Cloning
    • In-Fusion Cloning general information
      • In-Fusion Cloning overview
      • In-Fusion Cloning guide
      • In-Fusion Cloning and competition
        • In-Fusion Snap Assembly vs. GeneArt Gibson Assembly HiFi
        • In-Fusion Snap Assembly vs. NEBuilder HiFi
        • Sequence accuracy in seamless cloning
        • Choosing a seamless cloning method
        • Improving background over Gibson Assembly
        • A successful alternative to ligation cloning
        • Single- and multiple-insert cloning
        • Easy cloning into lentiviral vectors
        • Outperforming TOPO cloning
      • In-Fusion Cloning citations
      • Stellar Competent Cells product overview and performance data
      • EcoDry reagents and sustainability
    • Primer design and other tools
      • Seamless cloning primer design
      • In-Fusion Cloning tutorials
        • Cloning one or more fragments
        • Deleting a sequence
        • Inserting a sequence
        • Deleting and replacing a sequence
      • In-Fusion molar ratio calculator
      • Simulate your construct
    • In‑Fusion Cloning tips and FAQs
    • Applications and technical notes
      • In-Fusion Cloning applications collection
      • Efficient multiple-fragment cloning
      • Mutagenesis with In-Fusion Cloning
      • Rapid, high-throughput cloning for antibody development
      • Solve a synthesis challenge with easy multiple-insert cloning
      • Direct cloning into large vectors
      • Simplified insertion of a GFP-encoding cassette into a 100-kb plasmid
      • Efficient cloning for sgRNA/Cas9 plasmids
      • In-Fusion Cloning of sgRNAs
      • De novo insertion of small fusion protein tags
    • Sign up to stay updated
    • Traditional molecular cloning
      • Restriction enzyme overview
        • General information about restriction enzymes
        • Star activity of restriction enzymes
        • Inactivation of restriction enzymes
        • Buffer activity with restriction enzymes
        • Universal buffers for double digestion with restriction enzymes
        • Restriction enzymes affected by methylation
        • Methylation-sensitive restriction enzymes
        • QC of restriction enzymes
      • Ligation cloning overview
      • Ligation product guide
  • Nucleic acid purification
    • Nucleic acid extraction webinars
    • Product demonstration videos
    • Product finder
    • Plasmid kit selection guide
    • Plasmid purification
      • NucleoSpin Plasmid Transfection-grade
      • NucleoSnap Plasmid Midi
      • NucleoBond Xtra Midi/Maxi
    • Genomic DNA purification
      • Tissue
        • NucleoSpin Tissue
        • NucleoSpin DNA RapidLyse
        • NucleoSpin Tissue XS
        • NucleoMag DNA Swab
        • NucleoBond HMW DNA
        • NucleoSpin DNA Lipid Tissue
      • FFPE
        • NucleoMag DNA FFPE
        • NucleoSpin 96 DNA FFPE
      • Blood and plasma
        • NucleoMag Blood
        • NucleoSnap cfDNA
        • NucleoSpin cfDNA XS
        • NucleoMag cfDNA
        • NucleoSpin cfDNA Midi
      • Plant
        • NucleoMag 384 Plant
        • NucleoSpin Plant II
      • Other organisms and samples
        • NucleoMag DNA Bacteria
        • NucleoMag DNA Food
        • NucleoSpin DNA Insect
        • NucleoSpin Microbial DNA
    • DNA/RNA cleanup and extraction
      • NucleoSpin Gel and PCR XS product overview
      • NucleoSpin Gel and PCR Clean-up
      • NucleoMag NGS Clean-up and Size Select
      • NucleoSpin RNA Clean-up
    • RNA purification
      • RNA purification overview
      • NucleoSpin RNA Plus
      • NucleoSpin RNA Plus XS
      • NucleoSpin RNA
      • NucleoProtect RNA
      • NucleoSpin miRNA
      • NucleoSpin RNA Blood
      • NucleoSpin miRNA Plasma
      • NucleoZOL for RNA isolation
      • High-throughput RNA isolation from FFPE samples
      • NucleoSpin RNA Plant and Fungi
    • RNA purification kit finder
    • Viral DNA and RNA purification
      • Nucleic acid detection from wastewater
      • NucleoSpin VET
      • NucleoMag VET
      • NucleoMag Virus
      • NucleoMag Pathogen
    • Parallel DNA, RNA & protein
      • NucleoSpin RNA/Protein
      • NucleoSpin TriPrep
    • Automated DNA and RNA purification
      • Application notes, by sample type
        • Plasmid purification
        • DNA cleanup
        • DNA from cells and tissues
        • RNA from cells and tissues
        • DNA from blood and bodily fluids
        • DNA and RNA from plants, fungi, and food
        • Viral RNA and DNA purification
      • Application notes, by platform
        • Eppendorf
        • Tecan
        • Hamilton
        • Thermo Fisher Scientific
        • MASMEC Biomed
    • Accessory selection guides
      • Sample homogenization beads
      • NucleoBond racks selection guide
    • Microbiome
      • NucleoMag DNA Microbiome
      • NucleoSpin Soil
      • NucleoSpin 8/96 Soil
      • NucleoSpin DNA Stool
      • NucleoSpin 96 DNA Stool
      • NucleoMag DNA/RNA Water
      • NucleoSpin eDNA Water
      • NucleoBond RNA Soil
      • NucleoSpin RNA Stool
  • Antibodies and ELISA
    • Osteocalcin focus
  • Real-time PCR
    • Overview
      • One-step RT-qPCR kits
      • Two-step RT-qPCR kits
      • Probe-based qPCR kits
      • TB Green-based qPCR kits
      • Videos: qPCR analysis for challenging inputs
      • qRT-PCR to detect RNA present at low levels
    • Product finder
    • Reaction size guidelines
    • Real-time PCR products brochure
    • Real-time PCR tutorial videos
    • Guest webinar: extraction-free SARS-CoV-2 detection
    • Guest webinar: developing and validating molecular diagnostic tests
    • Technical notes
      • Unbiased preamplification of limited samples
      • Accurate gene expression analysis with TB Green Premix Ex Taq II
      • Efficient quantification of human gene expression with PrimeScript Reverse Transcriptase
      • Rapid qPCR analysis from blood samples using PrimeScript Reverse Transcriptase
      • Monitoring siRNA knockdown
      • qPCR without optimization using TB Green Premix Ex Taq
      • Fast synthesis of cDNA templates for real-time RT-PCR
      • Specific, consistent real-time PCR with TB Green Premix Ex Taq II
      • Mir-X microRNA quantification
    • FAQs
  • cDNA synthesis
    • Premium total and poly A+ RNA
    • SMARTer RACE 5'/3' Kit
    • Cloning antibody variable regions
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Stem cell learning center

stem cell cardiomyocytesStem cell research is at the forefront of biological discoveries and therapies. You not only need best-in-class tools, but also the support and knowledge of our scientists with over 15 years of stem cell experience. We offer a broad range of learning tools to help you take your research to the next level. Find application-specific protocols, read about our experimental data, watch educational videos, view cutting-edge posters from recent conferences, find published papers using our stem cell products, and more!


Stem cell protocols

Protocols and techniques for stem cell research.

Stem cell applications

Sign up to download application data on predicting intrinsic clearance using Cellartis Power Primary HEP Medium.

Stem cell technical notes

View data on our tools for stem cell research.

Stem cell citations

Citation lists using Takara Bio stem cell research tools.

Stem cell webinars

View webinars on a wide range of stem cell topics and applications

Stem cell videos

Short, educational videos about stem cell research.

Stem cell posters

Stem cell research posters from scientific conferences and meetings.

Stem cell selection guides

Find the right tools for your experiment using our stem cell selection guides.

Stem cell FAQs

Find answers to your stem cell research questions.

Stem cell overview

Overviews and discussions on a variety of stem cell research topics.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Diagnostic solutions
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • Services & Support
  • Instrument services
  • OEM & custom enzyme manufacturing
  • Stem cell services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Quality
  • Expertise
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • cDNA synthesis
  • Real-time PCR
  • Nucleic acid purification
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • Vaccine development
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
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