- Capturem technology
- Antibody immunoprecipitation
- His-tag purification
- Other tag purification
- Phosphoprotein and glycoprotein purification
- Matchmaker Gold yeast two-hybrid systems
- Expression systems
Bacterial expression overview
Takara Bio offers a choice of technologies for bacterial protein expression: standard E. coli expression, high-yield expression, low-temperature expression, high-purity expression, and bacterial expression systems.
Learn how to choose the best bacterial expression system for your specific application from our selection of standard E. coli expression systems, as well as specialized systems in E. coli or other bacterial hosts that are designed for high-yield, low-temperature, or high-purity expression.
|Selection guide for bacterial expression systems|
|Advantages||Protein yield per volume of culture||Applications|
|Standard E. coli expression systems|
|pET Express & Purify Kits||
||Up to 1 g/l||Inducible expression and purification of his-tagged proteins|
|HAT Protein Expression & Purification System||
||Up to 1 g/l||Expression and purification of HAT-tagged proteins|
|High-yield expression systems|
|B. subtilis Secretory Protein Expression System||
||Up to 25 g/l||Fermentation of secreted proteins (medium- and large-scale)|
|Brevibacillus Expression System||
||Up to 4 g/l||Expression of toxic proteins, proteins containing disulfide bonds, and his-tagged proteins (with four of the system vectors)|
|Low-temperature expression system|
|pCold DNA cold shock expression system||
||Several mg to several 10s of mg/l||Expression of insoluble proteins and his-tagged proteins (with most of the pCold vectors) in E. coli|
|High-purity expression system|
|SPP System (single-protein rroduction system)||
||Several 10s of mg/l||Expression of highly pure proteins, radioisotope labeling in E. coli|
Cloning shouldn't be work, it should just work ...
With In-Fusion technology, you will be able to perform directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction, with >95% cloning efficiency. Our In-Fusion bundles are complete systems that include all of the reagents you need for your cloning experiments: PCR enzyme, competent cells, and a kit for PCR cleanup or gel extraction. We have optimized the reagents to work together to give you the right clone the first time.In-Fusion Snap Assembly
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