mRNA synthesis learning center
In vitro transcription (IVT) is a molecular technique critical for expression studies (e.g., microinjection and in vitro translation), hybridizations, and structural or mechanistic studies. Explore below to learn about our in vitro transcription offerings and ways our technology can support your RNA research.
Takara IVTpro product overviews
Learn about a complete in vitro transcription solution.
5' capping information
Learn about co-transcriptional vs. post-transcriptional capping of mRNA
Webinar
Learn to learn how to generate ultra-high yields of synthetic mRNA.
FAQs
Helpful information and tips for successful in vitro transcription
Selection guide
Find the right product for your in vitro transcription workflow.
Download flyers
Learn about mRNA synthesis products.
Template generation | In vitro transcription | RNA purification | Poly(A) tailing | RNA capping | |
PCR & purification | Cloning & plasmid production | ||||
PrimeSTAR Max DNA Polymerase Ver.2 TaKaRa Ex Premier DNA Polymerase NucleoSpin Gel and PCR Clean-Up |
Takara IVTpro mRNA Synthesis System | ||||
Cloning Kit for mRNA Template | Takara IVTpro T7 mRNA Synthesis Kit | ||||
Template Vector (BspQ I) for T7 mRNA Synthesis | PrimeCap T7 RNA Polymerase (low dsRNA) | NucleoSpin RNA Clean-up | Poly(A) Polymerase | ||
In-Fusion Snap Assembly Master Mix | T7 RNA Polymerase ver.2.0 (High yield)* | ||||
Stellar Competent Cells | T7 RNA Polymerase, HQ† | ||||
NucleoSpin Plasmid | SP6 RNA Polymerase | ||||
BspQ I | Ribonucleoside triphosphates (rNTPs) | ||||
Recombinant RNase Inhibitor ver.2.0 | |||||
Pyrophosphatase (inorganic) | |||||
Pyrophosphatase (inorganic), HQ† |
*GMP available
†High Quality (HQ) grade refers to the quality level of the raw and ancillary materials to be used for further manufacturing. HQ grade reagents are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards.
GMP grade is a branding term Takara Bio uses to describe products manufactured at Takara Bio Inc. GMP-grade reagents are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards that operate in accordance with relevant Good Manufactured Practice (GMP) guidelines, as outlined in ICH guides or equivalent guidance documents or standards.
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