In vitro transcription
T7 RNA Polymerase ver.2.0
T7 RNA Polymerase ver.2.0 is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage T7 promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme uses double-stranded DNA containing T7 promoter sequence as a template and incorporates labeled or unlabeled nucleoside triphosphates to synthesize a single-stranded RNA transcript. Enhanced activity of T7 RNA Polymerase ver.2.0 enables the production of large quantities of high-quality RNA with high processivity and transcription frequency.
- Synthesis of capped mRNA using a cap analog
- Synthesis of long non-coding RNA or siRNA precursors
- Synthesis of RNA standard template for RT-qPCR
- Synthesis of RNA probes for hybridization
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Discover how our products are helping researchers overcome challenges in their vaccine development workflows.
Use our inorganic pyrophosphatase to improve the RNA yield in in vitro transcription reactions.
Takara IVTpro mRNA Synthesis System
A complete solution for synthesizing high-quality single-stranded mRNA containing your gene of interest with a cap structure and poly(A) sequence.
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