T7 RNA Polymerase ver.2.0
T7 RNA Polymerase ver.2.0 is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage T7 promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme uses double-stranded DNA containing T7 promoter sequence as a template and incorporates labeled or unlabeled nucleoside triphosphates to synthesize a single-stranded RNA transcript. Enhanced activity of T7 RNA Polymerase ver.2.0 enables the production of large quantities of high-quality RNA with high processivity and transcription frequency.
T7 RNA Polymerase ver.2.0 is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage T7 promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme uses double-stranded DNA containing T7 promoter sequence as a template and incorporates labeled or unlabeled nucleoside triphosphates to synthesize a single-stranded RNA transcript. Enhanced activity of T7 RNA Polymerase ver.2.0 enables the production of large quantities of high-quality RNA with high processivity and transcription frequency.
T7 RNA Polymerase, HQ (high quality) has been manufactured with stricter quality standards and is supplied in a higher volume (1 mL/tube) for scaling-up your processes. It is free from human- or animal-derived materials, as well as free from β-lactam compounds in the final formulation.
A GMP grade T7 RNA Polymerase is available upon request. Please contact us at inside_sales@takarabio.com for more information.
Do you want to explore OEM options? Contact OEM Services & Support at oem@takarabio.com.
For more information on the differences between research, HQ, and GMP grade enzymes, please refer to the HQ-grade reagents page.
More Information
Protocol
| RNase-free Water | up to 20 μl |
| 10X T7 RNA Polymerase Buffer | 2 μl |
| ATP, CTP, GTP, UTP | each 10 mM |
| Template DNA | 0.5–2 μg |
| Recombinant RNase Inhibitor ver.2.0 | 20 U |
| Pyrophosphatase (inorganic) | 0.1 U |
| T7 RNA Polymerase ver.2.0 | 200 U |
Incubate at 37°C for 1–2 hr.
Applications
- Synthesis of uncapped or capped mRNA using a cap analog
- Synthesis of long non-coding RNA or siRNA precursors
- Synthesis of guide RNAs
- Synthesis of RNA standard templates for RT-qPCR
- Synthesis of RNA probes for hybridization
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara IVTpro mRNA Synthesis System
A complete solution for synthesizing high-quality single-stranded mRNA containing your gene of interest with a cap structure and poly(A) sequence.
Inorganic Pyrophosphatase
Use our inorganic pyrophosphatase to improve the RNA yield in in vitro transcription reactions.
Oxidation-resistant recombinant RNase inhibitor
The oxidation-resistant Recombinant RNase Inhibitor ver.2.0 inhibits RNAse A and is suitable for use in enzymatic reactions requiring DTT concentrations, such as in vitro transcription, in vitro translation, and RT-PCR.
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