RNA sequencing

Good science leaves no possibility uninvestigated—no matter how small the sample size or how varied the sample composition. We offer best-in-class tools for rapid and accurate transcriptome analysis using RNA-seq (see selection guide below), and are continuously refining and building upon our core SMART (Switching Mechanism at the 5′ end of RNA Template) technology, which leverages the template-switching capability of certain reverse transcriptases to generate double-stranded cDNA in a single step.

We offer a wide variety of SMARTer RNA-seq kits: Our stranded total RNA-seq kits add priming sites for indexing PCR during reverse transcription, which means that Illumina-compatible sequencing libraries can be amplified after a single cleanup step. This approach streamlines your library preparation workflow by eliminating the need for A-tailing, ligation, and additional postligation cleanups. If your sample is mammalian, we provide a complete solution from total RNA to sequencing that includes rRNA depletion, cDNA synthesis, PCR amplification, and indexing primers. For other sample types, we provide kits that accommodate poly(A)-enriched, rRNA-depleted, or total RNA samples with the same ligation-free workflow. If you are using an alternative sequencing platform, we also provide cDNA synthesis kits that produce double-stranded cDNA that can be used directly as samples for other low-input DNA library prep kits.

Benefits

• Fast and simple—ligation-free SMART technology streamlines your RNA-seq library preparation regardless of input amount
• Accurate—identify each transcript's strand of origin with >99% accuracy for both mammalian and nonmammalian samples
• Sensitive—detect low-abundance transcripts, including noncoding RNAs
• Flexible—sequencer-agnostic cDNA synthesis kit doesn't limit your downstream approach
• Targeted—only sequence the transcripts you are interested in

Selection guide