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  • ‹ Back to Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
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Home › Applications › Cancer research › Cancer biomarker quantification

Cancer research

  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
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Overviews SmartChip system introduction

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Gene expression quantification for cancer biomarkers

Cancer Biomarker quantification

The primary method of diagnosing cancer and disease relies on lengthy, expensive, and complicated histological techniques, which are often employed in patients who are already symptomatic and thus further along in the disease state. Ideally, diagnosis methodologies should be rapid and efficient—enabling affordable and precise screening in presymptomatic, as well as symptomatic, populations. Nucleic acids, either from circulating fluids or FFPE tissue, have emerged as one potential category of biomarkers for studying cancer and disease. Clinically, nucleic acids have already been utilized for prenatal diagnostic testing, and numerous candidate tests are emerging for a variety of disease states. These biomarkers typically fall into four main categories: DNA, mRNA, microRNA (miRNA), and long noncoding RNA (lncRNA).

mRNA markers for many types of cancer, including liver, breast, and prostate have all been reported. However, the high degree of variability and turnover in mRNA makes it a challenging candidate for precision diagnostics. miRNA is a small (typically ~20 nt) species of noncoding RNA that is generally thought to regulate gene expression. Unlike coding mRNAs, miRNAs tend to be relatively stable and resistant to fragmentation. Currently, thousands of unique miRNAs have been identified, with implications across hundreds of diseases, including numerous types of cancers. lncRNAs are longer (typically >200 nt) transcripts that do not code for a specific protein. Similar to miRNA, lncRNA is typically thought to be involved in gene regulation. Hundreds of thousands of lncRNAs have been identified in multiple forms of cancer.

PCR, qPCR, and RT-qPCR are molecular techniques that allow for the detection and quantification of genes and gene expression in a variety of sample sources. These techniques allow for rapid, sensitive, and accurate detection of potential cancer biomarkers.

Highlighted products

Whole-genome SNP array with the Titanium DNA Amplification Kit

Cytogenetic microarrays offer a quick and cost-effective method for the detection of mutations in specific genes, including low-level mosaicism, loss of heterozygosity, and copy number change (Eldai et al. 2013). These mutations are known to affect the prognosis and treatment of various cancers such as melanoma, colorectal cancer, and acute lymphoblastic leukemia. Our Titanium DNA Amplification Kit has been used in microarray assays to screen for and discover mutations in various cancers, such as screening for BRAF mutations in solid tumors with the INFINITI BRAF Assay (Weyant et al. 2014) and uncovering IKFZ1 deletions in childhood acute lymphoblastic leukemia (Lopes et al. 2016). Finally, the high yield and consistent quality provided by the Titanium enzyme enables users of the Affymetrix GeneChip Mapping 500K Assay to achieve the very highest standards in SNP genotyping (Figure 1).

GeneChip Mapping 500K Assay workflow

Figure 1. Overview of the GeneChip Mapping 500K Assay.

Quantitative detection of gene expression using RT-qPCR and qPCR kits

qpcr curveRT-qPCR and qPCR allow for sensitive quantification of genes from RNA and gDNA samples from a variety of sources, including circulating tumor cells (Lianidou 2016). We have developed one-step RT-qPCR kits, two-step RT-qPCR kits, probe-based qPCR mixes, and TB Green-based qPCR mixes in a variety of user-friendly formats for rapid, accurate, and sensitive detection of genes of interest for cancer research. Additionally, as starting materials for cancer research may be precious (e.g., FFPE tissue), we developed our Takara PreAmp Master Mix for best-in-class, unbiased preamplification of as little as 12.5 pg of DNA.

These tools have been utilized to detect changes in TrkB signaling in frozen and FFPE uterine leiomyosarcoma samples (Makino et al. 2012), identify key miRNAs in 55 different breast cancer FFPE tissues (Chen et al. 2016), implicate MAF1 in primary human hepatocellular carcinoma tumors (Li et al. 2016), quantify downregulation of the SARI tumor suppressor in prostate cancer tissue and cells, accurately and specifically detect altered expression of RSK4 in multiple cell and tissue samples (Jiang et al. 2017), and ensure reproducible expression of 162 breast cancer-related genes in FFPE tissue samples (view tech note).

We have also developed lyophilized, prevalidated primer sets for colorectal cancer, pancreatic cancer, prostate cancer, melanoma, small cell lung cancer, and non-small cell lung cancer.

Sensitive miRNA quantification with Mir-X miRNA qRT-PCR TB Green kits 

Being able to accurately and sensitively quantify rare miRNA is critical to understanding and tracking the signaling network in various types of cancers (Hayes et al. 2014). Our Mir-X miRNA qRT-PCR TB Green kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry (Figure 2). This enables simple and accurate miRNA detection down to 50 copies from numerous sample types including plasma (Kanemaru et al. 2011), exosomes (Lee et al. 2013), and FFPE tissue (Lee et al. 2013).

Mir-X workflow

Figure 2. Mir-X miRNA qRT-PCR TB Green kit workflow.

High-throughput gene expression analysis with the SmartChip Real-Time PCR System

SmartChip systemOne of the challenges in screening for these biomarkers is the need to screen hundreds of potential targets in a variety of sample types. High-throughput qPCR has emerged as one potential solution, enabling rapid and sensitive detection of many targets. The SmartChip Real-Time PCR System has been utilized in numerous publications to profile miRNA (Choo et al. 2014), lncRNA (Leucci et al. 2016), and mRNA (Chen et al. 2016) in different forms of cancer and diseases from a multitude of sample types including blood, cell lines, T cells, plasma, and various types of patient biopsies. Critical to all of these studies was the ability of the SmartChip system to support numerous assay and sample configurations, as each disease has a unique and changing set of biomarkers. Through collaboration, we helped researchers design and utilize multiple panels for mRNA, miRNA, and lncRNA that could enable high-throughput screening for key biomarkers in their disease model of interest on the SmartChip system. To learn more about these panels and the research, visit the SmartChip system application pages.


References and product citations

Chen, X. et al. The role of miRNAs in drug resistance and prognosis of breast cancer formalin-fixed paraffin-embedded tissues. Gene 595, 221–226 (2016).

Chen, X. et al. Defining a population of stem-like human prostate cancer cells that can generate and propagate castration-resistant prostate cancer. Clin. Cancer Res. 22, 4505–4516 (2016).

Choo, K. B. et al. MicroRNA-5p and -3p co-expression and cross-targeting in colon cancer cells. J. Biomed. Sci. 21:95 (2014).

Eldai, H. et al. Novel genes associated with colorectal cancer are revealed by high resolution cytogenetic analysis in a patient specific manner. PLoS One 8, e76251 (2013).

Hayes, J., Peruzzi, P. P. & Lawler, S. MicroRNAs in cancer: biomarkers, functions and therapy. Trends Mol. Med. 20, 460–469 (2014).

Jiang, Y. et al. Aberrant expression of RSK4 in breast cancer and its role in the regulation of tumorigenicity. Int. J. Mol. Med. 40, 883–890 (2017).

Kanemaru, H. et al. The circulating microRNA-221 level in patients with malignant melanoma as a new tumor marker. J. Dermatol. Sci. 61, 187–193 (2011).

Lee, J.-K. et al. Exosomes derived from mesenchymal stem cells suppress angiogenesis by down-regulating VEGF expression in breast cancer cells. PLoS One 8, e84256 (2013).

Lee, T. S. et al. Aberrant microRNA expression in endometrial carcinoma using formalin-fixed paraffin-embedded (FFPE) tissues. PLoS One 8, e81421 (2013).

Leucci, E. et al. Melanoma addiction to the long non-coding RNA SAMMSON. Nature 531, 518–522 (2016).

Li, Y. et al. MAF1 suppresses AKT-mTOR signaling and liver cancer through activation of PTEN transcription. Hepatology 63, 1928–42 (2016).

Lianidou, E. S. Gene expression profiling and DNA methylation analyses of CTCs. Mol. Oncol. 10, 431–442 (2016).

Lopes, B. A. et al. COBL is a novel hotspot for IKZF1 deletions in childhood acute lymphoblastic leukemia. Oncotarget 7, 53064–53073 (2016).

Makino, K. et al. Inhibition of uterine sarcoma cell growth through suppression of endogenous tyrosine kinase B signaling. PLoS One 7, e41049 (2012).

Weyant, G. W. et al. BRAF mutation testing in solid tumors: a methodological comparison. J. Mol. Diagn. 16, 481–485 (2014).


Featured products

Cat. # Product Size License Quantity Details
639240 Titanium® DNA Amplification Kit 300 Rxns USD $1175.00

The Titanium DNA Amplification Kit is designed to be used with Affymetrix DNA Mapping products. This kit contains sufficient reagents for reactions of 100 μl each.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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PCR products

PCR products
PCR products. Examples of PCR products run on a 2% agarose gel at 120 V for 1 hr. Average product distribution is between ~250 bp and 1,100 bp.

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Overview of the GeneChip Mapping 500K Assay

Overview of the GeneChip Mapping 500K Assay
Overview of the GeneChip Mapping 500K Assay. The high yield and consistent quality provided by Clontech’s TITANIUM Taq DNA Polymerase enable users of the Affymetrix GeneChip Mapping 500K Assay to achieve the very highest standards in SNP genotyping.

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639240: Titanium DNA Amplification Kit

639240: Titanium DNA Amplification Kit
638541 Prelude™ PreAmp Master Mix 40 Rxns USD $557.00

Prelude PreAmp Master Mix uses an optimized 2X PCR mix for unbiased preamplification of over 100 targets, starting from a limited input of cDNA or gDNA (100 pg–10 ng). Amplified products can be used for downstream real-time PCR (qPCR), genotyping, or target enrichment analysis.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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The benefits of preamplification

The benefits of preamplification

Figure 1. The benefits of preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here. Data points for nonpreamplified genes with Ct >32 are not shown.

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Uniform representation following preamplification

Uniform representation following preamplification

Figure 2. Uniform representation following preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here.

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Uniformity following either 10 or 14 cycles of preamplification

Uniformity following either 10 or 14 cycles of preamplification

Figure 3. Uniformity following either 10 or 14 cycles of preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here.

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Delta-Delta Ct analysis demonstrates unbiased preamplification between 10 and 14 cycles

Delta-Delta Ct analysis demonstrates unbiased preamplification between 10 and 14 cycles

Figure 4. Delta-Delta Ct analysis demonstrates unbiased preamplification between 10 and 14 cycles. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here.

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Sensitive, unbiased preamplification using as little as 12.5 pg of starting material

Sensitive, unbiased preamplification using as little as 12.5 pg of starting material

Figure 5. Sensitive, unbiased preamplification using as little as 12.5 pg of starting material. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here.

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Takara PreAmp Master Mix provides less biased preamplification than other master mixes

Takara PreAmp Master Mix provides less biased preamplification than other master mixes

Figure 6. Takara PreAmp Master Mix provides less biased preamplification than other master mixes. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here.

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Takara PreAmp Master Mix provides highly unbiased preamplification when assaying 162 breast cancer targets in FFPE tissue

Takara PreAmp Master Mix provides highly unbiased preamplification when assaying 162 breast cancer targets in FFPE tissue

Figure 7. Takara PreAmp Master Mix provides highly unbiased preamplification when assaying 162 breast cancer targets in FFPE tissue.

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TaqMan PreAmp Master Mix displays more bias than Takara PreAmp Master Mix when assaying 162 breast cancer targets in FFPE tissue

TaqMan PreAmp Master Mix displays more bias than Takara PreAmp Master Mix when assaying 162 breast cancer targets in FFPE tissue

Figure 8. TaqMan PreAmp Master Mix displays more bias than Takara PreAmp Master Mix when assaying 162 breast cancer targets in FFPE tissue.

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638541: Prelude PreAmp Master Mix

638541: Prelude PreAmp Master Mix
RR037A PrimeScript™ RT Reagent Kit (Perfect Real Time) 200 Rxns USD $380.00

PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. This kit's protocol is simple and suitable for high throughput analysis. It can be used in combination with real-time PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A/B), TB Green Fast qPCR Mix (Cat. #RR430A/B), TB Green Premix Ex Taq (Tli RNaseH Pluse) (Cat. #RR420A/B), or Probe qPCR Mix (Cat. #RR391A/B) for two step real-time RT-PCR. The optimized protocol for assay can be selected in each assay condition using either TB Green or a probe.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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PrimeScript RT reagent Kit (Perfect Real Time) has the same high level of efficiency with various RT times (15 min., 30 min. or 60 min.) over a wide range of template concentrations

PrimeScript RT reagent Kit (Perfect Real Time) has the same high level of efficiency with various RT times (15 min., 30 min. or 60 min.) over a wide range of template concentrations
PrimeScript RT reagent Kit (Perfect Real Time) has the same high level of efficiency with various RT times (15 min., 30 min. or 60 min.) over a wide range of template concentrations.

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RR037A: PrimeScript RT Reagent Kit (Perfect Real Time)

RR037A: PrimeScript RT Reagent Kit (Perfect Real Time)
RR390A Premix Ex Taq™ (Probe qPCR) 200 Rxns USD $314.00

Premix Ex Taq (Probe qPCR) is a 2X premix for real-time PCR (qPCR) detection with probe-based qPCR or 5' nuclease assays. The 2X premix includes Takara Ex Taq HS, which contains a hot start PCR enzyme with an anti-Taq antibody, and a buffer optimized for real-time PCR. The resulting premix allows excellent suppression of non-specific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. In addition, Tli RNaseH, a heatresistant RNaseH, is included to minimize PCR inhibition from residual mRNA in reactions using cDNA templates. This product is excellent for high-speed PCR and allows accurate target quantification and detection over a broad dynamic range, making it possible to conduct highly reproducible and reliable real-time PCR analyses.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR390A: Premix Ex Taq (Probe qPCR)

RR390A: Premix Ex Taq (Probe qPCR)
639676 TB Green® Advantage® qPCR Premix 200 Rxns USD $205.00

TB Green Advantage qPCR Premix is a convenient 2X master mix containing TB Green dye, full-length Taq DNA Polymerase, hot-start antibody, dNTPs, and buffer (including Mg2+). The mix comes with two separate tubes of ROX passive reference dye: ROX Reference Dye LSR contains the optimal concentration of ROX for instruments whose excitation source is a 488 nm laser; and ROX Reference Dye LMP contains the optimal concentration of ROX for instruments whose excitation source is either a lamp or an LED.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Reaction specificity—performance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor R's qPCR mix using a Roche LightCycler.

Reaction specificity—performance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor R's qPCR mix using a Roche LightCycler.

Reaction specificity—performance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor R's  mix using a Roche LightCycler. The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor R's reagent are shown in Panels B and D. The cycling conditions for our reagent consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 95°C for 5 sec and 60°C for 20 sec. For Competitor R's reagent, the cycling conditions consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 94°C for 10 sec, 55°C for 5 sec, and 72°C for 10 sec.

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Amplification efficiency—performance of TB Green Advantage qPCR Premix vs. Competitor A's qPCR mix using an ABI PRISM 7000 Sequence Detection System.

Amplification efficiency—performance of TB Green Advantage qPCR Premix vs. Competitor A's qPCR mix using an ABI PRISM 7000 Sequence Detection System.

Amplification efficiency—performance of TB Green Advantage qPCR Premix vs. Competitor A's SYBR mix using an ABI PRISM 7000 Sequence Detection System. The results for the TB Green reagent are shown in Panels A and C, and those for Competitor A's reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95°C for 10 sec, followed by 40 cycles at 95°C for 5 sec and 60°C for 31 sec. For Competitor A's reagent, the cycling conditions consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 95°C for 15 sec and 60°C for 1 min.

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Reaction specificity—Takara Bio's TB Green Advantage qPCR Premix vs. Competitor I's qPCR mix using a Cepheid Smart Cycler.

Reaction specificity—Takara Bio's TB Green Advantage qPCR Premix vs. Competitor I's qPCR mix using a Cepheid Smart Cycler.

Reaction specificity—performance of Takara Bio's TB Green Advantage qPCR Premix vs. Competitor I's qPCR mix using a Cepheid Smart Cycler. The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor I's reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95°C for 2 min, followed by 45 cycles at 95°C for 5 sec and 60°C for 20 sec. For Competitor I's reagent, the cycling conditions consisted of 1 cycle at 95°C for 2 min, followed by 45 cycles at 95°C for 15 sec and 60°C for 30 sec.

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639676: TB Green Advantage qPCR Premix

639676: TB Green Advantage qPCR Premix
638319 Terra™ qPCR Direct TB Green® Premix 200 Rxns USD $268.00

Terra qPCR Direct TB Green Premix is a 2X master mix designed specifically for real-time PCR with TB Green chemistry. In addition to TB Green dye, this mix contains Terra qPCR Direct Polymerase, a novel enzyme developed for optimal amplification from crude or dirty templates; it’s perfect for amplifying short DNA targets (up to 2 kb), regardless of GC content or template purity. The premix also contains a monoclonal antibody that suppresses polymerase activity up to 98°C, allowing automatic hot start PCR.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Real-time PCR with crude extracts—Terra qPCR Direct TB Green Premix versus a conventional 2X qPCR premix

Real-time PCR with crude extracts—Terra qPCR Direct TB Green Premix versus a conventional 2X qPCR premix

Real-time PCR with crude extracts—Terra qPCR Direct TB Green Premix versus a conventional 2X qPCR premix. Real-time PCR was performed using undiluted, 4X diluted, and 16X diluted crude alkaline-heat extracts of mouse spleen or cow muscle (beef foodstuff), and either Terra qPCR Direct TB Green Premix or a conventional qPCR premix. Using the manufacturer's recommended conditions for each enzyme mix, a 165 bp region of the beta-globin gene Hbb-b1 was amplified from the mouse spleen extract (Panel A), and a 289 bp region of the cytochrome c oxidase gene (COX1) was amplified from the beef extract ( Panel B). Data generated by Terra qPCR Direct TB Green Premix corresponded to the theoretical quantities of each gene, while the conventional product was clearly affected by inhibitors present in the crude samples.

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Real-time PCR of GC-rich targets—Terra qPCR Direct TB Green Premix versus conventional 2X qPCR premixes

Real-time PCR of GC-rich targets—Terra qPCR Direct TB Green Premix versus conventional 2X qPCR premixes

Real-time PCR of GC-rich targets—Terra qPCR Direct TB Green Premix versus conventional 2X qPCR premixes. Targets with GC-content greater than 70% were amplified by real-time PCR using either human testis cDNA (equivalent to 50 ng–5 pg of total RNA; Panel A) or human genomic DNA (100 ng–10 pg; Panel B) as template, and either Terra qPCR Direct TB Green Premix (triangles) or one of two conventional SYBR premixes (one specifically for GC-rich targets; see figure legend). Using the manufacturer's recommended conditions for each enzyme mix, gene-specific primers were used to amplify portions of the jun-D proto-oncogene (JUND), the BTB domain-containing protein 6 gene (BTBD6), and the cyclin I gene (CCNI) from the cDNA (Panel A); and a portion of the beta-actin CpG island (ACTB_CpG) from the genomic DNA (Panel B). The resulting Ct values were plotted against the initial quantity of DNA used in each assay. Terra qPCR Direct TB Green Premix was the only premix able to consistently amplify all of the targets assayed.

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638319: Terra qPCR Direct TB Green Premix

638319: Terra qPCR Direct TB Green Premix
RR420A TB Green® Premix Ex Taq™ (Tli RNase H Plus) 200 Rxns USD $392.00

License Statement

ID Number  
M82 This product is covered by the claims of US patent No. 10,760,074 and its foreign counterparts.

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR420A: TB Green Premix Ex Taq (Tli RNase H Plus)

RR420A: TB Green Premix Ex Taq (Tli RNase H Plus)
RR820A TB Green® Premix Ex Taq™ II (Tli RNase H Plus) 200 Rxns USD $392.00

License Statement

ID Number  
M82 This product is covered by the claims of US patent No. 10,760,074 and its foreign counterparts.

TB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration premixed with TB Green at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent also contains Tli RNase H, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as template. This product, with a modified buffer composition, offers a higher reaction specificity than that of TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat.# RR420A). With inhibition of non-specific amplifications, which interfere with quantitative determination, accurate assays over a wide range are possible. A combination of this buffer and Takara Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, allows highly reproducible and reliable real time PCR analyses.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR820A: TB Green Premix Ex Taq II (Tli RNase H Plus)

RR820A: TB Green Premix Ex Taq II (Tli RNase H Plus)
638314 Mir-X™ miRNA qRT-PCR TB Green® Kit 200 Rxns USD $476.00

License Statement

ID Number  
269 For Research Use Only. Not for diagnostics. Not for use in diagnostic procedures. Product is covered by patents and patent applications owned by Exiqon A/S.

This 200 rxn kit enables you to synthesize first-strand cDNA from either total RNA or purified small RNAs isolated from any source, and use the resulting cDNA to quantify specific miRNAs and other RNAs present in the sample. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The TB Green Advantage qPCR Premix and real-time qPCR are used to quantify the sequences of choice in the cDNA. For 600 rxn kit see Cat. # 638316. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry. In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.

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Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants

Specific quantification of Let7 miRNA variants. Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A)+ RNA (Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.

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Induction and quantitation of miR-9 miRNA in mouse P19 cells

Induction and quantitation of miR-9 miRNA in mouse P19 cells
Induction and quantitation of miR-9 miRNA in mouse P19 cells. Exposing aggregated mouse P19 cell clusters to retinoic acid (RA) causes them to acquire neural cell phenotypes, which are accompanied by changes in the cellular miRNA pool. Using the Mir-X miRNA quantitation system, we tracked the abundance of one such miRNA, miR-9, which was induced by RA and continued to accumulate in these cells following a 5 day exposure to RA.

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Trichostatin A treatment alters miRNA expression in mouse ES cells

Trichostatin A treatment alters miRNA expression in mouse ES cells
Trichostatin A treatment alters miRNA expression in mouse ES cells. In mouse embryonic stem cells, we were able to monitor the alterations in expression for a panel of 12 miRNAs that respond to trichostatin A (TSA) treatment.

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638314: Mir-X miRNA qRT-PCR TB Green Kit

638314: Mir-X miRNA qRT-PCR TB Green Kit
640022 SmartChip® Real-Time PCR System Each Inquire for Quotation

License Statement

ID Number  
350 This product is protected by one or more of these U.S. Patents 7,622,296, 9,909,171, 9,132,427, 9,228,933 or 9,951,381, and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
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The SmartChip Real-Time PCR System offers high throughput genotyping and gene expression analysis in SmartChips which contain 5,184 individual nanowells. Samples and assays are dispensed into the SmartChip using the MutliSample NanoDispenser (MSND). Once the samples and assays have been dispensed, the SmartChip can be thermal cycled in the SmartChip Real-Time PCR Cycler.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640022: SmartChip Real-Time PCR System

640022: SmartChip Real-Time PCR System


SmartChip Real-Time PCR System

High-throughput real-time PCR

Where throughput meets flexibility

Real-time PCR (qPCR) is a powerful technique for genotyping and gene expression analysis. Currently, qPCR experiments are becoming increasingly complex—involving an expansive and growing list of targets from a larger number of samples, all with more technical replicates. The SmartChip Real-Time PCR System is a complete high-throughput solution that enables an unrivaled amount of flexible assay and sample formats, allowing researchers to seamlessly switch between dispensing assay reagents and samples into blank chips, or dispensing samples into custom, preprinted chips without the need for revalidation.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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