Tips and troubleshooting
There is nothing more frustrating than hitting a roadblock in the middle of your experiment. Our scientists have shared useful experimental design tips and troubleshooting tricks to help you overcome these hurdles and keep your experiments running smoothly. Check out our posts below and learn about common mistakes and how to avoid them.
Scaling up: moving from research to large-scale RNA production for mRNA therapeutics
Date: October 16, 2024
Explore the stages of mRNA therapeutics manufacturing—including synthesizing high-quality mRNA.
Why lyo-ready mixes are crucial for qPCR assay development
Date: September 26, 2024
Categories: Molecular Diagnostics
Lyophilized assays are highly stable, cost-effective, and easy to ship. Lyo-ready mixes are critical components of these assays, helping you simplify and streamline the development process while achieving your desired performance.
It's a snap! 9 considerations for easy multi-fragment cloning
Date: February 16, 2023
Categories: Troubleshooting
Learn best practices for multi-fragment cloning using Takara Bio's In-Fusion cloning system.
Best practices for RNA-seq success, part I: Optimizing sample prep
Date: December 22, 2021
In the first installment of our RNA-seq troubleshooting blog series, we go over the best practices of RNA sample prep, one of the most important steps in any NGS workflow.
Insertions, deletions, and substitutions, oh my!—designing primers for site-directed mutagenesis
Date: July 27, 2021
Categories: Troubleshooting
Design primers for generating deletions, base substitutions, or additions in any vector using a flexible, accurate system.
Top qualities to look for in your ideal OEM partner
Date: July 2, 2021
Categories: Useful resources
Finding the right OEM partner can be tricky. A good partner should be able to provide custom formulations, manage quality control and supply, and support upscaling and kitting. In this article, we help you make the right decision.
Understanding viral titration—behind the science
Date: March 25, 2020
Categories: Useful resources
A crash course on lentiviral titration methods to get your experiments started on the right foot.
4 factors to consider for immune repertoire profiling
Date: August 8, 2019
Categories: Immune research | Immunotherapy | NGS | Single-cell | Useful resources
Getting ready to sequence T-cell and B-cell receptors? Check out these tips to help you make key decisions before you start.
Top 5 tips for sorting (FACS) upstream of single-cell RNA-seq experiments
Date: July 24, 2019
Categories: NGS | Single-cell | Troubleshooting | Useful resources
Isolating single cells using FACS upstream of RNA-seq? These 5 tips will help you to succeed.
How to choose the right his-tagged purification resin
Date: June 17, 2019
Categories: Protein science | Useful resources
Learn how to decide between nickel- and cobalt-based IMAC resins for your his-tagged purification.
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