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639505
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Advantage® RT-for-PCR Kit |
25 Rxns |
USD $396.00
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Complete kit for the synthesis of first-strand cDNA from total RNA or polyA+ RNA. The kit contains sufficient reagents for 25 first-strand cDNA synthesis reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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639506
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Advantage® RT-for-PCR Kit |
100 Rxns |
USD $784.00
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Complete kit for the synthesis of first-strand cDNA from total RNA or polyA+ RNA. The kit contains sufficient reagents for 100 first-strand cDNA synthesis reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR023A
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BcaBEST™ RNA PCR Kit Ver. 1.1 |
100 Rxns |
USD $403.00
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The BcaBEST RNA PCR Kit is designed to perform both reverse transcription and DNA amplification in a single tube. The kit uses BcaBEST DNA Polymerase, which contains both DNA polymerase and reverse transcriptase for first strand cDNA synthesis. In contrast to standard reverse transcriptases, BcaBEST Polymerase has a temperature optimum of 65°C, which permits cDNA synthesis from difficult and highly structured RNA templates. Bca-Optimized Taq Polymerase is used for second strand synthesis and subsequent PCR. This polymerase utilizes LA PCR technology for improved PCR length and accuracy. Random 9-mers, oligo-dT primers, or a specific downstream primer that acts as an antisense primer in PCR can be used for cDNA synthesis.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR023B
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BcaBEST™ RNA PCR Kit Ver. 1.1 |
200 Rxns |
USD $686.00
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The BcaBEST RNA PCR Kit is designed to perform both reverse transcription and DNA amplification in a single tube. The kit uses BcaBEST DNA Polymerase, which contains both DNA polymerase and reverse transcriptase for first strand cDNA synthesis. In contrast to standard reverse transcriptases, BcaBEST Polymerase has a temperature optimum of 65°C, which permits cDNA synthesis from difficult and highly structured RNA templates. Bca-Optimized Taq Polymerase is used for second strand synthesis and subsequent PCR. This polymerase utilizes LA PCR technology for improved PCR length and accuracy. Random 9-mers, oligo-dT primers, or a specific downstream primer that acts as an antisense primer in PCR can be used for cDNA synthesis. Cat. # RR023B contains 2 of Cat. # RR023A. Please refer to Cat. # RR023A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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637404
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Clontech® PCR-Select™ Bacterial Genome Subtraction Kit |
7 Rxns |
USD $1166.00
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Complete kit for comparing two bacterial genomes (from two different species of bacteria or two different strains of the same species) and obtaining DNA fragments that are present in one genome but not in the other. Enough reagents are provided for one control and six complete subtractions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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637401
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Clontech® PCR-Select™ cDNA Subtraction Kit |
7 Rxns |
USD $1166.00
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Kit for identifying cDNAs that correspond to differentially expressed sequences in one cDNA population compared with another. Enough reagents are provided for seven cDNA reactions. PCR primers are provided for 50 primary and 100 secondary PCR amplifications—enough for one control and six complete subtractions if the cDNA from each synthesis is used for tester and driver in separate subtractions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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637402
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Clontech® PCR-Select™ Differential Screening Blocking Solution |
1 mL |
USD $338.00
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Hybridization Blocking Solution for use with the Clontech PCR-Select Differential Screening Kit.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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637403
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Clontech® PCR-Select™ Differential Screening Kit |
Each |
USD $641.00
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Complete kit for differential screening of subtracted libraries obtained using the Clontech PCR-Select cDNA Subtraction Kit. The differential screening procedure can be used to identify putative differentially expressed sequences in the subtracted library before performing Northern blot analysis. The kit contains reagents for making cDNA probes for differential screening and hybridization control cDNAs.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634933
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In-Fusion® SMARTer® Directional cDNA Library Construction Kit |
10 Rxns |
USD $1496.00
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The In-Fusion SMARTer Directional cDNA Library Construction Kit provides a sensitive and efficient method for producing high-quality, full-length cDNA libraries. The kit utilizes two of our most innovative technologies: SMART (Switching Mechanism At 5' end of RNA Transcript) technology coupled with PCR amplification makes it possible to generate high yields of full-length double-stranded cDNA from nanograms of poly A+ or total RNA. In-Fusion cloning technology makes it easy to clone your SMARTer cDNA library into any location within any vector. You may use the pSMART2IFD Linearized Vector included in the kit, or any vector of your choice, for cloning your library. Isolated clones from finished libraries can be transferred directly to any linearized expression vector for functional analysis-without the need for compatible restriction sites.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634913
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Marathon® cDNA Amplification Kit |
100 Rxns |
USD $849.00
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This kit contains reagents for making adaptor-ligated ds cDNA from poly A+ RNA and performing 5' and 3' Marathon RACE PCR using gene-specific primers and a mixture of thermostable DNA polymerases supplied by the user. This kit contains sufficient reagents for 5 cDNA synthesis reactions and 100 PCR reactions.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR024A
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One-Step RNA PCR Kit (AMV) |
50 Rxns |
USD $446.00
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Takara Bio's One Step RNA PCR Kit offers the convenience and time savings of performing reverse transcription and PCR in a single tube, without the need for changing buffers or adding reagents, minimizing the risk of contamination. AMV Reverse Transcriptase XL provides greater thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. The kit also contains AMV-Optimized Taq DNA Polymerase for efficient amplification of the cDNA synthesized by AMV Reverse Transcriptase XL.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR024B
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One-Step RNA PCR Kit (AMV) |
100 Rxns |
USD $882.00
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Takara's One Step RNA PCR Kit offers the convenience and time savings of performing reverse transcription and PCR in a single tube, without the need for changing buffers or adding reagents, minimizing the risk of contamination. AMV Reverse Transcriptase XL provides greater thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. The kit also contains AMV-Optimized Taq DNA Polymerase for efficient amplification of the cDNA synthesized by AMV Reverse Transcriptase XL. Cat. # RR024B contains 2 of Cat. # RR024A. Please refer to Cat. # RR024A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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635003
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pEXP-Lib Vector |
20 ug |
USD $384.00
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pEXP-Lib Vector contains an internal ribosome entry site (IRES), which permits the translation of two open reading frames from one messenger RNA. After selection with puromycin, nearly all surviving colonies will stably express the gene of interest, thus decreasing the need to screen large numbers of colonies to find functional clones. SfiIA and SfiIB indicate two distinct SfiI sites that differ in their interpalindromic sequences. This arrangement permits cloning of libraries such that all inserts are in the proper orientation for protein translation.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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635002
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pRetro-Lib Vector |
20 ug |
USD $358.00
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pRetro-Lib, derived from Moloney murine leukemia virus (MoMuLV), is designed for delivery and expression of retroviral libraries or genes. Upon transfection into a packaging cell line, pRetro-Lib can transiently express, or integrate and stably express, a transcript containing (psi, the extended viral packaging signal) and the gene of interest. The 5' viral LTR in this vector contains a viral promoter that controls expression of genes cloned into the MCS.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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6110A
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PrimeScript™ 1st strand cDNA Synthesis Kit |
50 Rxns |
USD $323.00
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The PrimeScript 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize first strand cDNA from total or polyA+ RNA using PrimeScript RTase, a MMLV (Moloney Murine Leukemia Virus)-derived reverse transcriptase. This enzyme is capable of synthesizing cDNA up to 12 kb in length. PrimeScript RTase can synthesize cDNA efficiently at 42℃, even from RNA templates that contain GC-rich regions or complex secondary structures, making it unnecessary to perform reactions at high temperatures that may degrade RNA. The first strand cDNA synthesized with this kit can be used for variety of applications, including second strand synthesis, hybridization, PCR amplification, and real-time PCR.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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6110B
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PrimeScript™ 1st strand cDNA Synthesis Kit |
200 Rxns |
USD $1085.00
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The PrimeScript 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize first strand cDNA from total or polyA+ RNA using PrimeScript RTase, a MMLV (Moloney Murine Leukemia Virus)-derived reverse transcriptase. This enzyme is capable of synthesizing cDNA up to 12 kb in length. PrimeScript RTase can synthesize cDNA efficiently at 42℃, even from RNA templates that contain GC-rich regions or complex secondary structures, making it unnecessary to perform reactions at high temperatures that may degrade RNA. The first strand cDNA synthesized with this kit can be used for variety of applications, including second strand synthesis, hybridization, PCR amplification, and real-time PCR. Cat. # 6110B contains 4 of Cat. # 6110A. Please refer to Cat. # 6110A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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6111A
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PrimeScript™ Double Strand cDNA Synthesis Kit |
10 Rxns |
USD $844.00
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cDNA cloning is used for structural analysis of genes and RNAs, and expression of target proteins. Generating a cDNA library involves synthesizing double-stranded cDNA that is complementary to the target mRNA, inserting the cDNA into a vector of bacterial or viral origin, and introducing the vector into bacterial or eukaryotic cells. The cloned cDNA can be then used for analysis, and in vitro transcription and translation. The PrimeScript Double Strand cDNA Synthesis Kit synthesizes double-stranded cDNA from polyA+ RNA of animal or plant origin using the Gubler-Hoffman method.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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R022A
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PrimeScript™ High Fidelity RT-PCR Kit |
50 Rxns |
USD $385.00
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The PrimeScript High Fidelity RT-PCR Kit is designed to synthesize and amplify cDNA from total RNA or mRNA with high fidelity. This kit includes all reagents necessary for reverse transcription of RNA to cDNA followed by PCR amplification of cDNA, including PrimeScript reverse transcriptase and PrimeSTAR Max DNA Polymerase.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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R022B
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PrimeScript™ High Fidelity RT-PCR Kit |
200 Rxns |
USD $1083.00
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The PrimeScript High Fidelity RT-PCR Kit is designed to synthesize and amplify cDNA from total RNA or mRNA with high fidelity. This kit includes all reagents necessary for reverse transcription of RNA to cDNA followed by PCR amplification of cDNA, including PrimeScript reverse transcriptase and PrimeSTAR Max DNA Polymerase. Cat. # R022B contains 4 of Cat. # R022A. Please refer to Cat. # R022A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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6215A
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PrimeScript™ IV 1st strand cDNA Synthesis Mix |
50 Rxns |
USD $348.00
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PrimeScript IV 1st strand cDNA Synthesis Mix contains a 5X premix for synthesizing first-strand cDNA from total RNA or poly A+ RNA. A cDNA synthesis reaction can be started simply by adding template RNA and water to the premix, which already contains PrimeScript IV Reverse Transcriptase, RNase Inhibitor, Oligo dT Primer, dNTP Mixture, and reaction buffer. The premix also contains an accessory protein that eliminates the need for RNA denaturation, which is commonly performed before reverse transcription.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR055A
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PrimeScript™ One Step RT-PCR Kit Ver.2 |
50 Rxns |
USD $397.00
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The PrimeScript One Step RT-PCR Kit Ver.2 is designed for RT-PCR in a single tube. All components necessary for RT-PCR are mixed in one tube, and therefore it is unnecessary to add reagents at the midpoint of the procedure and the risk of contamination is minimized. This kit includes PrimeScript 1 step Enzyme Mix (a highly optimized premix of PrimeScript Reverse Transcriptase, which has excellent extension even for RNA templates containing higher-order structure, the high efficiency hot-start PCR enzyme Takara Ex Taq HS, and RNase Inhibitor together with a stabilizing agent) and 2X 1 step buffer (a premix containing reaction buffer, dNTP mixture, and 1 step Enhancer Solution). These premix components allow easy preparation of reaction mixtures. This kit facilitates efficient production of RT-PCR products through efficient reverse transcription at 50°C by permitting reaction conditions and reduction in non-specific amplification that can arise from mispriming or from primer-dimer during pre-cycling steps. This kit includes all reagents necessary for reverse transcription of RNA to cDNA and cDNA amplification using PCR.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR055B
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PrimeScript™ One Step RT-PCR Kit Ver.2 |
200 Rxns |
USD $1263.00
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The PrimeScript One Step RT-PCR Kit Ver.2 is designed for RT-PCR in a single tube. All components necessary for RT-PCR are mixed in one tube, and therefore it is unnecessary to add reagents at the midpoint of the procedure and the risk of contamination is minimized. This kit includes PrimeScript 1 step Enzyme Mix (a highly optimized premix of PrimeScript Reverse Transcriptase, which has excellent extension even for RNA templates containing higher-order structure, the high efficiency hot-start PCR enzyme Takara Ex Taq HS, and RNase Inhibitor together with a stabilizing agent) and 2X 1 step buffer (a premix containing reaction buffer, dNTP mixture, and 1 step Enhancer Solution). These premix components allow easy preparation of reaction mixtures. This kit facilitates efficient production of RT-PCR products through efficient reverse transcription at 50°C by permitting reaction conditions and reduction in non-specific amplification that can arise from mispriming or from primer-dimer during pre-cycling steps. This kit includes all reagents necessary for reverse transcription of RNA to cDNA and cDNA amplification using PCR. Cat. # RR055B contains 4 of Cat. # RR055A. Please refer to Cat. # RR055A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR057A
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PrimeScript™ One Step RT-PCR Kit, Ver.2 (Dye Plus) |
50 Rxns |
USD $397.00
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The PrimeScript One Step RT-PCR Kit Ver.2 (Dye Plus) is used for one-step RT-PCR in a single tube. There is no need to add reagents during the reaction, minimizing the risk of contamination. Furthermore, electrophoresis loading dyes (blue and yellow) are included in the premix to allow direct analysis by electrophoresis. The bright green color of the reaction mixture allows visual confirmation of gel loading. The kit includes PrimeScript RTase, a reverse transcriptase that provides superior elongation, and Takara Ex Taq HS, a PCR enzyme formulated for hot start PCR that provides high yields. The reaction components are optimized to provide the same level of performance as conventional RT-PCR systems that lack dyes and loading buffer. This kit includes all of the reagents necessary for the synthesis of cDNA from RNA by reverse transcription and amplification of cDNA by PCR.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR057B
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PrimeScript™ One Step RT-PCR Kit, Ver.2 (Dye Plus) |
200 Rxns |
USD $1263.00
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The PrimeScript One Step RT-PCR Kit Ver.2 (Dye Plus) is used for one-step RT-PCR in a single tube. There is no need to add reagents during the reaction, minimizing the risk of contamination. Furthermore, electrophoresis loading dyes (blue and yellow) are included in the premix to allow direct analysis by electrophoresis. The bright green color of the reaction mixture allows visual confirmation of gel loading. The kit includes PrimeScript RTase, a reverse transcriptase that provides superior elongation, and Takara Ex Taq HS, a PCR enzyme formulated for hot start PCR that provides high yields. The reaction components are optimized to provide the same level of performance as conventional RT-PCR systems that lack dyes and loading buffer. This kit includes all of the reagents necessary for the synthesis of cDNA from RNA by reverse transcription and amplification of cDNA by PCR. Cat. # RR057B contains 4 of Cat. # RR057A. Please refer to Cat. # RR057A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR014A
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PrimeScript™ RT-PCR Kit |
50 Rxns |
USD $321.00
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This kit for two-step RT-PCR is designed to provide excellent extendibility and efficient amplification. It uses PrimeScript reverse transcriptase and Takara Ex Taq HS polymerase. This kit generates RT-PCR products efficiently at standard reverse transcription temperature (42℃), even when RNA templates contain higher-order structures. It is not necessary to perform reactions at a high temperature when there is a risk of RNA decomposition. This kit includes all reagents necessary for the reverse transcription of RNA to cDNA, and for cDNA amplification using PCR.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR014B
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PrimeScript™ RT-PCR Kit |
200 Rxns |
USD $1067.00
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This kit for two-step RT-PCR is designed to provide excellent extendibility and efficient amplification. It uses PrimeScript reverse transcriptase and Takara Ex Taq HS polymerase. This kit generates RT-PCR products efficiently at standard reverse transcription temperature (42℃), even when RNA templates contain higher-order structures. It is not necessary to perform reactions at a high temperature when there is a risk of RNA decomposition. This kit includes all reagents necessary for the reverse transcription of RNA to cDNA, and for cDNA amplification using PCR. Cat. # RR014B contains 4 of Cat. # RR014A. Please refer to Cat. # RR014A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR012A
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RNA LA PCR™ Kit (AMV) Ver. 1 |
100 Rxns |
USD $746.00
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The RNA LA PCR Kit is designed to generate longer and more accurate RT-PCR products. The kit uses AMV RT XL as the reverse transcriptase, which allows for efficient synthesis of first-strand cDNA up to 12 kb. AMV RT XL also provides better thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. In addition, the kit uses LA Taq, which can synthesize products up to 40 kb in size with 6.5X better fidelity than Taq Polymerase. Both the reverse transcription and amplification reactions can be performed in a single tube. The components for 3'-RACE are also supplied.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR019A
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RNA PCR Kit (AMV) Version 3.0 |
100 Rxns |
USD $482.00
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The RNA PCR Kit (AMV) Version 3.0 is designed for performing single tube reverse transcription of RNA to cDNA using AMV (Avian Myeloblastosis Virus) Reverse Transcriptase, with subsequent cDNA amplification using Takara Ex Taq HS. Taking advantage of AMV RTase XL, which possesses higher thermostability and a broader range of reaction temperatures (42–60°C) than MMLV RTase, this kit allows transcription at higher temperatures, which eliminates potential RNA secondary structure that can give rise to polymerase pauses. The supplied Oligo dT-Adaptor Primer is designed for highly efficient cDNA synthesis from the poly(A)+ RNA 3'-terminus, which allows amplification of unknown 3'-termini using 3'-RACE. The components for the 3'-RACE method are supplied in the kit.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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RR019B
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RNA PCR Kit (AMV) Version 3.0 |
200 Rxns |
USD $938.00
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The RNA PCR Kit (AMV) Version 3.0 is designed for performing single tube reverse transcription of RNA to cDNA using AMV (Avian Myeloblastosis Virus) Reverse Transcriptase, with subsequent cDNA amplification using Takara Ex Taq HS. Taking advantage of AMV RTase XL, which possesses higher thermostability and a broader range of reaction temperatures (42-60°C) than MMLV RTase, this kit allows transcription at higher temperatures, which eliminates potential RNA secondary structure that can give rise to polymerase pauses. The supplied Oligo dT-Adaptor Primer is designed for highly efficient cDNA synthesis from the poly(A)+ RNA 3'-terminus, which allows amplification of unknown 3'-termini using 3'-RACE. The components for the 3'-RACE method are supplied in the kit. Cat. # RR019B contains 2 of Cat. # RR019A. Please refer to Cat. # RR019A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634901
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SMART® cDNA Library Construction Kit |
Each |
USD $1213.00
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A kit for making seven cDNA libraries from nanogram amounts of total or poly A+ RNA. The kit utilizes the proprietary SMART (Switching Mechanism At 5' end of RNA Template) Oligonucleotide to generate high yields of full-length ds cDNA. Control Poly A+ RNA, Control Insert and pre-digested λTriplEx2 are included.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634925
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SMARTer® PCR cDNA Synthesis Kit |
10 Rxns |
USD $876.00
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A kit for performing ten cDNA syntheses from nanogram amounts of total or poly A+ RNA. The kit utilizes our SMART (Switching Mechanism At 5' end of RNA Template) technology coupled with PCR amplification to generate high yields of full-length double-stranded cDNA suitable for various applications, including Clontech PCR-Select cDNA subtraction, nondirectional cloning, and preparation of complex cDNA probes. The SMARTer PCR cDNA Synthesis Kit includes the same components as the original SMART PCR cDNA Synthesis Kit plus an improved SMARTer II A oligo and SMARTScribe Reverse Transcriptase, which provide higher specificity and increased yield.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634926
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SMARTer® PCR cDNA Synthesis Kit |
20 Rxns |
USD $1402.00
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A kit for performing twenty cDNA syntheses from nanogram amounts of total or poly A+ RNA. The kit utilizes our SMART (Switching Mechanism At 5' end of RNA Template) technology coupled with PCR amplification to generate high yields of full-length double-stranded cDNA suitable for various applications, including Clontech PCR-Select cDNA subtraction, nondirectional cloning, and preparation of complex cDNA probes. The SMARTer PCR cDNA Synthesis Kit includes the same components as the original SMART PCR cDNA Synthesis Kit plus an improved SMARTer II A oligo and SMARTScribe Reverse Transcriptase, which provide higher specificity and increased yield.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634928
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SMARTer® Pico PCR cDNA Synthesis Kit |
10 Rxns |
USD $1348.00
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The SMARTer Pico PCR cDNA Synthesis Kit provides a PCR-based method for producing high-quality cDNA from picogram quantities of total RNA. The cornerstone of SMARTer cDNA synthesis is still our unique SMART (Switching Mechanism At the 5' End of RNA Transcript) technology; now with a modified SMARTer II A oligo. This new and improved oligo, combined with SMARTScribe Reverse Transcriptase, provides higher specificity and increased yields. The SMARTer Pico PCR cDNA Synthesis Kit allows you to synthesize high-quality cDNA for array probe generation, cDNA subtraction, "Virtual Northern" blots, or other applications, from as little as 1 ng of total RNA at extremely low concentrations (or in diluted samples). It is especially useful for researchers who have limited starting material, such as RNA derived from laser-capture microscopy samples, cells sorted by flow cytometry, or other extremely small samples.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634858
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SMARTer® RACE 5’/3’ Kit |
10 Rxns |
USD $801.00
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The SMARTer RACE 5'/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (Switching Mechanism At 5' End of RNA Template) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. Our carefully designed, specially-modified SMARTer Oligo preferentially captures the 5' ends of the cDNA during cDNA synthesis. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences, thus increasing the likelihood you will amplify the entire sequence of your gene. RACE PCR products are amplified with the provided SeqAmp DNA Polymerase, and cloned into the linearized pRACE vector with In-Fusion HD Cloning. The SMARTer RACE 5'/3' Kit has been improved to accommodate larger RNA input volumes and perform better on challenging targets than the original SMARTer RACE cDNA Amplification Kit. The In-Fusion HD Cloning Kit, NucleoSpin Gel and PCR Clean-Up Kit, and Stellar Competent Cells are included for your convenience in cloning RACE products. Gene-specific RACE primers are supplied by the user.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634859
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SMARTer® RACE 5’/3’ Kit |
20 Rxns |
USD $1353.00
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The SMARTer RACE 5'/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (Switching Mechanism At 5' End of RNA Template) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. Our carefully designed, specially-modified SMARTer Oligo preferentially captures the 5' ends of the cDNA during cDNA synthesis. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences, thus increasing the likelihood you will amplify the entire sequence of your gene.
RACE PCR products are amplified with the provided SeqAmp DNA Polymerase, and cloned into the linearized pRACE vector with In-Fusion HD Cloning. The SMARTer RACE 5'/3' Kit has been improved to accommodate larger RNA input volumes and perform better on challenging targets than the original SMARTer RACE cDNA Amplification Kit. The In-Fusion HD Cloning Kit, NucleoSpin Gel and PCR Clean-Up Kit, and Stellar Competent Cells are included for your convenience in cloning RACE products. Gene specific RACE primers are supplied by the user.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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639503
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Titanium® One-Step RT-PCR Kit |
30 Rxns |
USD $268.00
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The Titanium One-Step RT-PCR Kit is designed for the reverse transcription of total or poly A+ RNA and the subsequent PCR amplification of a target cDNA in a single reaction tube. The included enzyme mix contains a highly purified Moloney murine leukemia virus (MMLV) reverse transcriptase for first-strand cDNA synthesis, and Titanium Taq DNA Polymerase for PCR amplification. Titanium Taq DNA Polymerase is a blend of a 5'-exonuclease deficient, thermostable DNA polymerase, and TaqStart Antibody for automatic hot start PCR. Reagents for purifying RNA are not supplied.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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639504
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Titanium® One-Step RT-PCR Kit |
100 Rxns |
USD $621.00
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The Titanium One-Step RT-PCR Kit is designed for the reverse transcription of total or poly A+ RNA and the subsequent PCR amplification of a target cDNA in a single reaction tube. The included enzyme mix contains a highly purified Moloney murine leukemia virus (MMLV) reverse transcriptase for first-strand cDNA synthesis, and Titanium Taq DNA Polymerase for PCR amplification. Titanium Taq DNA Polymerase is a blend of a 5'-exonuclease deficient, thermostable DNA polymerase, and TaqStart Antibody for automatic hot start PCR. Reagents for purifying RNA are not supplied.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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634922
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Universal Primer Mix |
100 Rxns |
USD $152.00
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The Universal Primer Mix (UPM) adds suppression PCR elements to the ends of cDNA generated by our SMARTer RACE 5'/3' Kit (Cat. Nos. 634858 & 634859). The UPM consists of two primers: a long, 45-base primer and a short, 22-base primer. The Long Universal Primer (Long UP) is composed of two parts: the 23 bases at the 3' end of the primer are identical to those at the 5' end of the SMARTer IIA Oligo; the 22 bases at the 5' end of the primer provide the suppression sequence for suppression PCR. The Short Universal Primer (Short UP) sequence is identical to that of the 22 bases at the 5' end of the Long UP. The Short UP, for which the binding site is introduced by the Long UP during the first round of PCR, is present in the UPM at 5 times the concentration of the Long UP.
Notice to purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
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