Nucleic acid purification and fractionation: CHROMA SPIN columns
CHROMA SPIN columns are packed with gel filtration resin and enable rapid purification and size selection of nucleic acid samples. The columns can be used to purify single- or double-stranded DNA or RNA from contaminants such as salts, solvents, enzymes, or proteins. Molecules larger than the particular matrix pore size are eluted out of the column while smaller molecules are retained inside the column matrix. As a result, these columns are ideal for size fractionation of libraries or for removal of primers.
CHROMA SPIN columns are packed with gel filtration resin and enable rapid purification and size selection of nucleic acid samples. The columns can be used to purify single- or double-stranded DNA or RNA from contaminants such as salts, solvents, enzymes, or proteins. Molecules larger than the particular matrix pore size are eluted out of the column while smaller molecules are retained inside the column matrix. As a result, these columns are ideal for size fractionation of libraries or for removal of primers.
Available in three different sterile buffers
- TE buffer: ideal for routine DNA applications
- STE (0.1 M NaCl + TE) buffer: used for applications requiring a higher salt concentration to prevent ionic interaction between molecules
- DEPC-treated water (+ 0.1 mM EDTA): useful for RNA purification and applications requiring a completely nuclease-free environment
Choose from six different column pore sizes
CHROMA SPIN-10, -30, -100, -200, -400, and -1000. All columns, matrices, and buffers have been autoclaved to destroy nucleases. For optimal recovery and purification, there should be at least a threefold difference between the size of the desired nucleic acid molecules and the size of the contaminants.
Overview
- Adaptable—one system for any application
- Efficient—purify samples in 10 minutes
- Simple—apply sample and spin
More Information
Applications
- Nucleic acid purification
- Removal of contaminants
- Buffer exchange
- Library construction
- Microarray spotting
- PCR
- Cloning
Principal CHROMA SPIN column applications
CHROMA SPIN Series |
Purify oligos/primers after labeling reactions (bases) |
DNA/RNA lengths (bases) |
Remove primers/linkers digestion fragments (bases) |
Remove proteins/enzymes (kDa) |
CHROMA SPIN-10 |
>15 |
— |
— |
— |
CHROMA SPIN-30 |
— |
>35 |
<9 |
<30 |
CHROMA SPIN-100 |
— |
>140 |
<30 |
<250 |
CHROMA SPIN-200 |
— |
>300 |
<50 |
<1,000 |
CHROMA SPIN-400 |
— |
>600 |
<100 |
<8,000 |
CHROMA SPIN-1000 |
— |
>1,350 |
<300 |
all sizes |
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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