BspQ I

BspQ I creates linearized, scarless templates from plasmid DNA that is suitable for use in in vitro transcription.

Recognition Site:

5’. . .GCTCTTCN|. . .3’
3’. . .CGAGAAGNNNN|. . .5’

BspQ I creates linearized, scarless templates from plasmid DNA that is suitable for in in vitro transcription.

Recognition Site:

5’. . .GCTCTTCN|. . .3’
3’. . .CGAGAAGNNNN|. . .5’

As a type IIS restriction enzyme that cleaves DNA outside its recognition site, the use of BspQ I to linearize plasmid DNA ensures that the 3′ end of the in vitro transcription template does not contain extra base pairs from the restriction site. This keeps the template poly(A) sequence intact and promotes successful translation after mRNA synthesis.

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Cat. #	Product	Size
1227A	BspQ I	500 U	USD $89.00
BspQ I is a type IIS restriction enzyme that creates linearized, scarless templates from plasmid DNA that are suitable for in vitro transcription. BspQ I (10 U/μl) and 10X BspQ I Buffer are included. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back BspQ I Restriction Site BspQ I cuts outside of its recognition site, which keeps the poly(A) tail sequence intact. Back 1227A: BspQ I
1227B	BspQ I	2,500 U	USD $394.00
BspQ I is a type IIS restriction enzyme that creates linearized, scarless templates from plasmid DNA that are suitable for in vitro transcription. BspQ I (10 U/μl) and 10X BspQ I Buffer are included. Cat. # 1227B contains 5 of Cat. # 1227A. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back BspQ I Restriction Site BspQ I cuts outside of its recognition site, which keeps the poly(A) tail sequence intact. Back 1227B: BspQ I

Overview

Perfect for preparing scarless in-vitro transcription templates from plasmid DNA

Cuts outside recognition site keeping the poly(A) tail sequence intact, which promotes successful translation downstream of the in vitro transcription
Linearizes plasmid DNA in one hour

More Information

Applications

Linearization of plasmid DNA for use as a template for in vitro transcription.

Source

Escherichia coli carrying the plasmid containing the gene for BspQ I.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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BspQ I

Notice to purchaser

BspQ I Restriction Site

1227A: BspQ I