Seeker Spatial Transcriptomics Kit resources

Simple, single-day workflow plugs directly into existing sequencing workflows. Once your tissue section is placed on the Seeker tile, mRNA is captured and hybridized on the spatially-indexed beads before reverse transcription. The indexed beads are then dissociated from the tile and the tissue is digested, followed by second strand synthesis and cDNA amplification. Purified cDNA undergoes NGS library preparation and is sequenced. The resulting FASTQ files are processed by the Seeker Analysis Pipeline to create a continuous whole transcriptome spatial map of your tissue section.

The Seeker kit produces gene expression maps with sharp spatial boundaries using tiles available in two convenient sizes: 3 mm x 3 mm and 10 mm x 10 mm. The Seeker Analysis Pipeline mapped spatial gene expression within a mouse hippocampus section (top row) and whole mouse brain (bottom row). Expression patterns of the top five spatially variable genes are shown.