Primer design and other tools
Takara Bio provides easy-to-use tools to help you plan your cloning experiments:
- Design primers for single- or multi-insert cloning or for your site-directed mutagenesis experiment (insertion, deletion, replacement) with our primer design tool
- Calculate the optimal amounts of vector and insert for your cloning reaction with our molar ratio calculator
- Simulate your In-Fusion Cloning construct with SnapGene software
Design your primers
NEW TOOL!
Our NEW In-Fusion Cloning Primer Design Tool allows for single- or multiple-insert cloning, accommodates vector linearization by inverse PCR or restriction digest, and enables site-directed mutagenesis. Simply input the DNA sequences of your vector and insert(s), along with your linearization method to generate primers for your next cloning experiment. Easily switch to the mutagenesis option to generate primers for all of your insertion, replacement, and deletion projects.
Launch »
Seamless cloning primer design tips
Useful tips to keep in mind when designing your seamless cloning projects.
In-Fusion Cloning primer design tutorials
Learn how to use our online tool to quickly and easily design primers for every In-Fusion Cloning project.
Molar ratio calculator for In-Fusion Cloning
Calculate the optimal amounts of vector and insert for an In-Fusion Cloning reaction. Just enter the sizes of both vector and insert(s), in base pairs.
Simulate your In-Fusion Cloning construct
SnapGene software allows easy planning and visualization of molecular biology procedures, including In-Fusion Cloning.
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