In-Fusion Cloning citations

In-Fusion Cloning has been used to investigate and develop tools against COVID-19 and the SARS-CoV-2 virus that causes it.

• An infectious cDNA clone of a growth attenuatedKorean isolate of MERS coronavirus KNIH002 inclade B. Read now »
• Cross-reactive Antibody Response between SARS-CoV-2 and SARS-CoV Infections. Read now »
• Cutting Edge: Mouse SARS-CoV-2 Epitope Reveals Infection and Vaccine-Elicited CD8 T Cell Responses. Read now »
• Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza. Read now »
• Functional assessment of cell entry and receptor usage for lineage B β-coronaviruses, including 2019-nCoV. Read now »
• Neutralization of SARS-CoV-2 by destruction of the prefusion spike. Read now »
• Neutralizing antibodies targetingthe SARS-CoV-2 receptor binding domain isolated fromanaïve human antibody library. Read now »
• Newcastle disease virus (NDV) expressing the spike protein of SARS-CoV-2 as a live virus vaccine candidate. Read now »
• Stability of RNA sequences derived from the coronavirus genome in human cells. Read now »
• Ubiquitin ligase RIPLET mediates polyubiquitination of RIG-I and LGP2 and regulates the innate immune responses to SARS-CoV-2 infection. Read now »

In-Fusion Cloning has been used for seamless cloning of sgRNA constructs for CRISPR/Cas9 vector systems.

• Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza. Read now »
• Microhomology-assisted scarless genome editing in human iPSCs. Read now »
• A highly efficient ligation-independent cloning system for CRISPR/Cas9 based genome editing in plants. Read now »
• Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system. Read now »
• MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Read now »
• The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation. Read now »
• Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Read now »
• Efficient genome editing in the oomycete Phytophthora sojae using CRISPR/Cas9. Read now »
• Targeted mutagenesis of guinea pig Cytomegalovirus using CRISPR/Cas9-mediated gene editing. Read now »

In-Fusion Cloning has been used for streamlined, high-throughput cloning in antibody engineering workflows.

• Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells. Read now »
• Creation of antigen-dependent β-lactamase fusion protein tethered by circularly permuted antibody variable domains. Read now »
• Efficient generation of monoclonal antibodies from single rhesus macaque antibody secreting cells. Read now »
• Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis. Read now »

In-Fusion Cloning has been used for cloning inserts directly into large, complex lentiviral vectors.

• Gene expression profiling reveals U1 snRNA regulates cancer gene expression. Read now »
• Single cell resolution in vivo imaging of DNA damage following PARP inhibition. Read now »
• Lrig1 is a haploinsufficient tumor suppressor gene in malignant glioma. Read now »
• Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors. Read now »

In-Fusion Cloning has been used to investigate the mechanisms of various human diseases.

• Oxidative stress inhibits distant metastasis by human melanoma cells. Read now »
• Rb suppresses human cone-precursor-derived retinoblastoma tumours. Read now »
• Visualization and targeting of LGR5⁺ human colon cancer stem cells. Read now »
• DNA methylation analysis of the EGR3 gene in patients of schizophrenia. Read now »

In-Fusion Cloning has been used to study embryogenesis and development in different species.

• Live imaging of cellular dynamics during Caenorhabditis elegans postembryonic development. Read now »
• DUX-family transcription factors regulate zygotic genome activation in placental mammals. Read now »

In-Fusion Cloning has been used to study gene regulation, protein interactions, and metabolic pathways in various microorganisms, as well as viral infection of mammalian cells.

• Revisiting the regulation of the primary scaffoldin gene in Clostridium thermocellum. Read now »
• Yeast dynamin interaction with ESCRT proteins at the endosome. Read now »
• A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea. Read now »
• Interaction between HCMV pUL83 and human AIM2 disrupts the activation of the AIM2 inflammasome. Read now »

In-Fusion Cloning has been used to study gene regulation, growth mechanisms, viral infections, and stress response in plants.

• Shoot-to-root mobile polypeptides involved in systemic regulation of nitrogen acquisition. Read now »
• Co-option of a photoperiodic growth-phase transition system during land plant evolution. Read now »
• Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase. Read now »
• Construction of Agrobacterium tumefaciens-mediated tomato black ring virus infectious cDNA clones. Read now »
• DoGMP1 from Dendrobium officinale contributes to mannose content of water-soluble polysaccharides and plays a role in salt stress response. Read now »
• A highly efficient ligation-independent cloning system for CRISPR/Cas9-based genome editing in plants. Read now »

In-Fusion Cloning has been adapted to high-throughput cloning platforms to generate many constructs in parallel.

• A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Read now »
• Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells. Read now »
• High throughput generation and characterization of replication-competent clade C transmitter-founder simian human immunodeficiency viruses. Read now »