We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About

Close

  • ‹ Back to Learning centers
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
    • In-Fusion Cloning tools
    • In-Fusion Cloning guide
    • In‑Fusion Cloning FAQs
    • In‑Fusion Cloning tips
    • Choosing a seamless cloning method
    • Seamless cloning primer design
    • In-Fusion Cloning applications collection
    • Efficient cloning for sgRNA/Cas9 plasmids
    • In-Fusion Cloning tech notes
    • In-Fusion Cloning citations
    • EcoDry reagents and sustainability
    • Mutagenesis with In-Fusion Cloning
    • Efficient multiple-fragment cloning
    • Sign up to stay updated
    • Traditional molecular cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
Save on In-Fusion Cloning starter kits 50% off In-Fusion Cloning starter kits!
In-Fusion Cloning kits In-Fusion Cloning kits
Download the In-Fusion cloning infographic Download our infographic about seamless cloning
In-Fusion Cloning Interest Sign up for In-Fusion updates and promotions
Home › Learning centers › Cloning

Learning centers

  • Automation systems
    • SmartChip Real-Time PCR System introduction
      • SmartChip Real-Time PCR System overview
      • SmartChip Real-Time PCR System technical specifications
      • SmartChip Real-Time PCR System applications
        • Antibiotic resistance genes
          • Antibiotic resistance genes in manure and sewage
          • Antibiotic resistance genes in soil, sediment, and sludge
          • Antibiotic resistance genes in hospitals
          • Antibiotic resistance genes in water
        • mRNA, miRNA, and lncRNA as disease biomarkers
        • Pathogen detection in human samples and food
        • Genotyping using animal and blood samples
      • SmartChip Real-Time PCR System video resources
        • Antibiotic resistance screening webinar
      • Request a SmartChip system demo
    • Apollo library prep system introduction
      • Automated VeriSeq library preparation for PGS
      • Apollo library prep system citations
      • SMART-Seq v4 chemistry for the SMARTer Apollo system
      • Apollo library prep system overview
      • Apollo system technical specifications
      • In-tip bead separation on the Apollo system
    • ICELL8 introduction
      • ICELL8 technology overview
      • ICELL8 cx technical specifications
      • ICELL8 technical specifications (original system)
      • Technical notes
        • ICELL8 cx system reagent formulation and dispense guidelines
        • Protocol: High-throughput single cell DNA-seq
        • Improved detection of gene fusions, SNPs, and alternative splicing
        • Full-length transcriptome analysis
        • Protocol: High-throughput single-cell ATAC-Seq
        • High-throughput single-cell ATAC-seq
        • Single-cell identification with CellSelect Software
        • Single-cell analysis elucidates cardiomyocyte differentiation from iPSCs
        • Combined TCR profiling and 5’ DE in single cells
        • Automated, high-throughput TCR profiling
      • Sample preparation protocols
        • Protocol: Nuclei isolation from mammalian cells
        • Protocol: Mouse cardiomyocyte preparation
        • Isolate cells of any size
      • Video resources
      • Posters
      • Citations
  • Next-generation sequencing
    • Selection guide
    • Product line overview
    • Technical notes
      • Single-cell RNA- and DNA-seq
        • Highest sensitivity for single-cell mRNA-seq
        • Stranded libraries from single cells
        • Streamlined single-cell mRNA-seq
        • Single-cell mRNA-seq
        • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
        • Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
        • Full-length single-cell library method comparison
        • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
        • Next-gen WGA method for CNV and SNV detection from single cells
      • RNA-seq
        • Stranded libraries from picogram-input total RNA (v2)
        • Stranded libraries from FFPE inputs (v2)
        • Stranded libraries from 100 ng - 1 ug total RNA
        • Stranded libraries from 100 pg-100 ng total RNA
        • Stranded libraries from picogram-input total RNA (v1)
        • Stranded RNA-seq competitor kit comparison
        • Nonstranded libraries from FFPE inputs
        • Sensitive capture of full-length transcript information with targeted RNA-seq
      • DNA-seq
        • Low cell number ChIP-seq using SMARTer ThruPLEX
        • Cell-free DNA sequencing
        • Sequencing analysis of low-frequency mutations in cfDNA
        • DNA-seq from FFPE samples
        • Low-input whole-exome sequencing
        • Tag-seq variant detection
        • Low-volume DNA shearing for SMARTer ThruPLEX library prep
      • Immune profiling
        • TCR repertoire profiling from human samples (single cells)
        • TCR repertoire profiling from human samples (bulk)
        • BCR repertoire profiling from human samples (bulk)
        • TCR repertoire profiling from mouse samples (bulk)
        • BCR repertoire profiling from mouse samples (bulk)
      • Epigenetics and smRNA-seq
        • Full-length small RNA libraries
        • ChIP-seq libraries for transcription factor analysis
        • ChIP-seq libraries from ssDNA
        • Methylated DNA-seq
    • Featured kits
    • Technology and application overviews
      • SMART technology
      • Total RNA-seq
      • Whole genome amplification from single cells
      • Cell-free nucleic acid sequencing
      • Sequencing depth for SMARTer ThruPLEX Tag-seq
    • FAQs and tips
      • RNA-seq FAQs
      • RNA-seq tips
      • Positive and negative controls in scRNA-seq
      • DNA-seq FAQs
      • ChIP-seq FAQs
    • DNA-seq protocols
      • Exome capture with Illumina Nextera Rapid Capture
      • Targeted capture with Roche NimbleGen SeqCap EZ
      • Targeted capture with IDT xGen panels
      • Targeted capture with Agilent SureSelectXT
      • Targeted capture with Agilent SureSelectXT2
      • Targeted capture with Agilent SureSelectQXT
      • Plasma preparation for ThruPLEX Plasma-Seq workflow
    • Webinars
      • Sign up: automating full-length single-cell RNA-seq libraries
      • Sign up: single-cell DNA-seq webinar
      • Sign up: single-cell whole transcriptome analysis
      • Single-cell RNA-seq
      • Total RNA-seq
      • Immune profiling
      • Epigenetics
      • Targeted RNA-seq
      • DNA-seq
    • Citations
      • Epigenetics citations
      • Microbiome citations
      • SMARTer PicoPLEX citations
      • SMARTer RNA-seq citations
      • ThruPLEX DNA-seq citations
      • ThruPLEX Plasma-seq citations
    • Posters
  • Gene function
    • Gene editing
      • Gene editing product finder
      • Gene editing tools and information
        • sgRNA design tools
        • Tools for successful CRISPR/Cas9 genome editing
        • Gene editing posters
        • Customer data for Guide-it products
        • How to design sgRNA sequences
        • Introduction to the CRISPR/Cas9 system
        • Gene editing of CD3+ T cells and CD34+ HSCs
      • CRISPR/Cas9 knockouts
        • Mutation detection kit comparison
        • Screening for effective guide RNAs
        • Monoallelic versus biallelic mutants
        • Indel identification kit for mutation characterization
      • CRISPR/Cas9 knockins
        • Choosing an HDR template format
        • Homology-directed repair FAQs
        • Mouse CRISPR knockin protocol
        • Site-specific gene knockins using long ssDNA
        • Efficient CRISPR/Cas9-mediated knockins in iPS cells
      • Genome-wide screening
        • CRISPR library screening
        • CRISPR library screening webinar
        • Phenotypic screen using sgRNA library system
      • Creating and screening for SNPs
        • SNP detection with knockin screening kit
        • Oligo design tool for SNP screening
          • Oligo design tool user guide
        • Sign up: SNP engineering webinar
        • Guide-it SNP Screening Kit FAQs
      • CRISPR/Cas9 delivery methods
        • Electroporation-grade Cas9 for editing in diverse cell types
        • CRISPR/Cas9 gene editing with AAV
        • CRISPR/Cas9 gesicles overview
        • Cas9 Gesicles—reduced off-target effects
        • sgRNA-Cas9 delivery to many cell types
        • Tet-inducible Cas9 for gene editing
      • Cre recombinase
        • Control your Cre recombinase experiments
        • Fast Cre delivery with gesicle technology
    • Viral transduction
      • Recombinant virus comparison
      • Product finder
      • Transduction posters
      • Lentivirus
        • Lenti-X FAQs
        • Lentiviral workflow
        • Lentiviral products guide
        • Lentivirus biosafety
        • Lentiviral tips
        • Lentiviral vectors
        • Lenti-X packaging
        • Lenti-X Concentrator
        • Lentiviral titration
        • Lenti-X GoStix Plus video protocols
        • Lenti-X GoStix Plus FAQs
        • Rapid lentivirus titration by p24 ELISA
        • Lentiviral particles
        • Lentiviral particles—fluorescent
        • Lentiviral particles webinar
        • Lentiviral particles FAQs
      • Retrovirus
        • Retroviral products
        • Retro-X FAQs
        • Retro-X packaging
        • Retro-X Concentrator
      • Adeno-associated virus
        • AAV workflow
        • AAV delivery in vivo
        • AAV products
        • AAV FAQs
        • AAV2 purification video
        • AAVpro Concentrator overview
      • Adenovirus
        • Adenoviral FAQs
        • Adenoviral products
        • Fastest, easiest adenoviral system ever
        • Tet-inducible adenovirus
        • Adenovirus purification kits
        • Adenovirus purification mega-scale
        • Adenovirus rapid titer
        • Adenoviral titration
    • T-cell transduction and culture
      • Technology overview
      • Adoptive T-cell therapy (ACT)
      • RetroNectin FAQs
      • Hematopoietic cell transduction
      • T-cell expansion
      • Serum-free T-cell culture
      • CultiLife culture bags protocol
    • Inducible systems
      • iDimerize systems
        • Inducible protein-protein interactions—iDimerize systems
        • iDimerize systems journal club
        • iDimerize in vivo protocol
        • iDimerize systems citations
        • ARGENT cell signaling regulation kits from ARIAD
      • ProteoTuner systems
        • ProteoTuner technology overview
        • ProteoTuner citations
        • Inducible protein stabilization systems product selection guide
      • Tet-inducible systems
        • Tet systems product selection guide
        • Tet systems overview
        • Tet-One technology overview
        • Tet-On 3G plasmid system kit components
        • Tet-On 3G lentiviral system kit components
        • Tet system webinars
    • Transfection reagents
      • Xfect Transfection Reagent
      • Transfection tips
      • Cell lines transfected with the Xfect reagent
      • Protein transfection FAQs
      • Xfect RNA Transfection Reagent
      • Transfecting with single shots video
      • Single shots picture protocol
      • Transfection reagent products
      • Protocol: transfection of cerebellar slice cultures with Xfect reagent
      • Calcium phosphate transfection of neurons
    • Fluorescent proteins
      • Fluorescent protein vector finder
      • Fluorescent protein excitation and emission maxima
      • Fluorescent protein antibodies selection guide
      • Fluorescent protein antibody citations
        • GFP antibody citations
        • RFP antibody citations
      • Fluorescent protein quick guide
      • Fluorescent retroviral expression vectors
      • Verify miRNA expression
      • Find EGFP vector alternatives
  • Stem cell research
    • Applications
    • Technical notes
      • Pluripotent stem cells
        • Using the DEF-CS system to culture human iPS cells
        • Comparison of the Cellartis DEF-CS system with other vendors' human iPS cell culture systems
        • Reprogramming PBMCs
        • Reprogramming fibroblasts
      • Gene editing in hiPS cells
        • Tagging an endogenous gene with AcGFP1 in hiPS cells
        • Tagging an endogenous gene with a myc tag in hiPS cells
        • Generating clonal hiPS cell lines deficient in CD81
        • Introducing a tyrosinemia-related SNP in hiPS cells
        • Inserting an expression cassette into the AAVS1 locus in hiPS cells
        • Editing hiPS cells using electroporation
        • Editing hiPS cells using gesicle technology
        • Single-cell cloning of hiPS cells
      • Beta cells
        • Beta cells for disease modeling
      • Hepatocytes
        • hiPS-HEP cells for disease modeling
        • hiPS-HEP cells for drug metabolism studies
        • Power medium for long-term human primary hepatocyte culture
        • iPS cell to hepatocyte differentiation system
      • Cardiomyocytes
        • Making engineered heart tissue with cardiomyocytes
      • Neural stem cells
        • RHB-A neural stem cell medium
    • Posters
    • Webinars
      • Edited hiPS cell lines for disease modeling
      • Power medium for intrinsic clearance studies
    • Videos
      • Hepatocyte offerings
    • Protocols
      • Getting started with hepatocyte differentiation
      • Cardiomyocytes in FLIPR 384-well plate format
      • Cardiomyocytes on the Patchliner system
      • Cardiomyocytes on the Maestro MEA system
      • Cardiomyocytes on the MED64 MEA system
      • Cardiomyocytes on the CardioExcyte 96 system
      • Cardiomyocytes on the xCELLigence RTCA CardioECR system
      • Reprogramming fibroblasts
      • Reprogramming PBMCs
      • Spin embryoid body formation
      • Transferring iPSCs from other media to DEF-CS
      • Transferring iPSCs on MEFs to DEF-CS
    • FAQs
      • Cellartis DEF-CS 500 Culture System FAQs
      • Cellartis enhanced hiPS-HEP FAQs
      • Cellartis iPS Cell to Hepatocyte Differentiation System FAQs
    • Citations
      • Cellartis Power Primary HEP Medium
      • Cellartis DEF-CS 500 Culture System
      • Cellartis Enhanced hiPS-HEP
      • Cellartis hES-MP 002.5
      • Cellartis hPS cell-derived cardiomyocytes
      • Cellartis iPS Cell to Hepatocyte Differentiation System
      • GS1-R
      • GS2-M
      • iMatrix-511
      • iSTEM
      • NDiff 227
      • NDiff N2
      • RHB-A
      • STEM101
      • STEM121
      • STEM123
    • Selection guides
      • Stem cell antibody selection guide
      • Stem cell media product finder
      • Stem cell tools product finder
      • Hepatocyte product finder
    • Overview
      • Stem cell research products and services
      • Stem cell media products
      • Cardiomyocyte products
      • Hepatocyte products and services
      • iPS cell to hepatocyte differentiation overview
      • Cellartis human pluripotent stem cell services
  • Protein research
    • Capturem rapid purification technology
      • FAQs about Capturem technology
      • Capturem posters
      • Rapid antibody labeling with Capturem technology
      • Capturem citations
    • Antibody purification
      • Tech note: Rapid Protein G-based antibody purification
      • Tech note: thiophilic antibody purification resins
      • Tech note: Purification and screening
      • Tech note: IP and Co-IP
    • His-tag purification
      • Why tag a protein?
      • Tech note: cobalt resin
      • Purification methods overview
      • Simplified purification of active, secreted his-tagged proteins
      • Overview: His60
      • Tech note: Capturem technology
      • Tech note: Capturem large volume
      • Magnetic beads
      • TALON resin selection guide
      • Selection guide: His60 resin
      • FAQs: TALON
      • Protocols
        • Video: Capturem his maxiprep
        • Video: Capturem his miniprep
        • Visual protocol: Capturem his maxiprep
        • Visual protocol: Capturem his miniprep
        • Capturem nickel column reagent compatibility
        • TALON reagent compatibility
        • His60 reagent compatibility
        • TALON: Native vs denaturing purification
        • Protocol: denaturing purification with TALON resin, imidazole elution
        • Protocol: native purification with TALON resin, imidazole elution
        • Protocol: native purification with TALON resin, pH elution
    • Other tag purification
      • Streptavidin-based enrichment using Capturem technology
      • Selection guide: peptide tags
      • Myc-tagged protein purification overview
      • GST-tagged protein purification overview
    • Phosphoprotein and glycoprotein purification
      • Non-tagged protein purification overview
      • Phosphoprotein purification overview
    • Mass spectrometry digestion reagents
      • Capturem technology for trypsin protein digestion
      • Protein and whole-cell lysate digestion with Capturem Trypsin technology
      • Protein digestion with Capturem Pepsin
    • Matchmaker Gold yeast two-hybrid systems
      • Matchmaker Gold Yeast Two-Hybrid System
      • Make your own library for yeast two-hybrid screening
      • Mate and Plate yeast two-hybrid cDNA libraries
      • Aureobasidin A for improved selectable drug resistance in yeast
    • Expression systems
      • Protein expression overview
      • Insect expression overview
      • Mammalian expression overview
      • pHEK293 Ultra expression overview
      • OKT3 expression in mammalian cells
      • Bacterial expression overview
  • PCR
    • Citations
      • PrimeSTAR HS
      • EmeraldAmp MAX
      • Terra PCR Direct
      • EmeraldAmp GT
      • Takara Ex Taq
      • PrimeSTAR Max
      • PrimeSTAR GXL
      • Takara LA Taq
      • SpeedSTAR HS
      • Takara Taq and Taq HS
      • Titanium Taq
    • Selection guides
      • PCR selection guide
      • Direct PCR selection guide
      • GPR-registered PCR products selection guide
      • Commercial-use PCR selection guide
      • Fast PCR selection guide
    • PCR enzyme brochure
    • Technical notes
      • Rapid, high-performance multiplex PCR
      • EmeraldAmp outperforms MyTaq Red mix
      • Fast and accurate PCR
      • Methylation studies
      • Hot-start PCR
      • Long-range PCR with LA Taq
      • Direct PCR from human nail
      • Direct PCR from meat samples
      • Megaprimer PCR with PrimeSTAR GXL
      • Amplifying GC-rich templates
      • Titanium Taq for high-throughput genotyping
      • Colony PCR in under an hour
      • High-throughput endpoint PCR
      • Direct PCR from blood
      • PrimeSTAR GXL for targeted sequencing
      • Detecting somatic mosaicism using massively parallel sequencing
    • PCR FAQs
      • Primer design
      • Optimization
      • Troubleshooting
      • Applications and conditions
      • Shipping, storage, and handling
    • Go green with lyophilized enzymes
  • Cloning
    • In-Fusion Cloning tools
      • In-Fusion molar ratio calculator
      • Simulate your construct
    • In-Fusion Cloning guide
    • In‑Fusion Cloning FAQs
    • In‑Fusion Cloning tips
    • Choosing a seamless cloning method
    • Seamless cloning primer design
    • In-Fusion Cloning applications collection
    • Efficient cloning for sgRNA/Cas9 plasmids
    • In-Fusion Cloning tech notes
      • Simplified insertion of a GFP-encoding cassette into a 100-kb plasmid
      • In-Fusion Cloning of sgRNAs
      • Sequence accuracy in seamless cloning
      • De novo insertion of small fusion protein tags
      • Improving background over Gibson Assembly
      • Rapid, high-throughput cloning for antibody development
      • A successful alternative to ligation cloning
      • Single- and multiple-insert cloning
      • Direct cloning into large vectors
      • Easy cloning into lentiviral vectors
      • Outperforming TOPO cloning
      • Solve a synthesis challenge with easy multiple-insert cloning
    • In-Fusion Cloning citations
    • EcoDry reagents and sustainability
    • Mutagenesis with In-Fusion Cloning
    • Efficient multiple-fragment cloning
    • Sign up to stay updated
    • Traditional molecular cloning
      • Restriction enzyme overview
        • General information about restriction enzymes
        • Star activity of restriction enzymes
        • Inactivation of restriction enzymes
        • Buffer activity with restriction enzymes
        • Universal buffers for double digestion with restriction enzymes
        • Restriction enzymes affected by methylation
        • Methylation-sensitive restriction enzymes
        • QC of restriction enzymes
      • Ligation cloning overview
      • Ligation product guide
  • Nucleic acid purification
    • Product finder
    • Plasmid purification
      • Plasmid kit selection guide
      • NucleoSpin Plasmid Transfection-grade
      • NucleoSnap Plasmid Midi
      • NucleoBond Xtra Midi/Maxi
    • Genomic DNA purification
      • NucleoSpin Tissue
      • NucleoSpin DNA RapidLyse
      • NucleoSpin Tissue XS
      • NucleoSnap DNA Plasma
      • NucleoSpin Plasma XS
      • NucleoMag DNA Plasma
      • NucleoMag Pathogen
      • NucleoMag Virus
      • NucleoMag Blood
      • NucleoBond HMW DNA
      • NucleoMag DNA FFPE
      • NucleoSpin 96 DNA FFPE
      • NucleoMag DNA Swab
      • NucleoMag VET
      • NucleoMag 384 Plant
      • NucleoSpin DNA Plasma Midi
    • DNA/RNA cleanup and extraction
      • NucleoSpin RNA Clean-up
      • NucleoSpin Gel and PCR Clean-up
    • Parallel DNA, RNA & protein
      • NucleoSpin RNA/Protein
      • NucleoSpin TriPrep
    • Automated DNA and RNA purification
      • Application notes, by sample type
        • Plasmid purification
        • DNA cleanup
        • DNA from cells and tissues
        • RNA from cells and tissues
        • DNA from blood and bodily fluids
        • DNA and RNA from plants, fungi, and food
        • Viral RNA and DNA purification
      • Application notes, by platform
        • Eppendorf
        • Tecan
        • Hamilton
        • Thermo Fisher Scientific
        • MASMEC Biomed
    • RNA purification
      • RNA purification overview
      • RNA purification kit finder
      • NucleoSpin RNA Plus
      • NucleoSpin RNA Plus XS
      • NucleoSpin RNA
      • NucleoProtect RNA
      • NucleoSpin miRNA
      • NucleoSpin RNA Blood
      • NucleoSpin miRNA Plasma
      • NucleoZOL for RNA isolation
      • High-throughput RNA isolation from FFPE samples
    • Hard-to-lyse samples
      • NucleoSpin Plant II
      • NucleoSpin RNA Plant and Fungi
      • NucleoMag DNA Food
      • NucleoSpin DNA Insect
      • NucleoSpin Soil
      • NucleoSpin 8/96 Soil
      • NucleoBond RNA Soil
      • NucleoSpin DNA Lipid Tissue
      • NucleoSpin DNA Stool
      • NucleoSpin RNA Stool
      • NucleoSpin Microbial DNA
      • NucleoMag DNA/RNA Water
      • Sample homogenization beads
  • Antibodies and ELISA
    • Osteocalcin focus
  • Cell biology assays
    • Extracellular vesicle isolation from biofluids
    • Cell viability kits
    • Exosome isolation from cell culture
    • Mir-X microRNA quantification
  • Real-time PCR
    • Product finder
    • Reaction size guidelines for qPCR
    • Real-time PCR products brochure
    • Real-time PCR tutorial videos
    • Overview
      • One-step RT-qPCR kits
      • Two-step RT-qPCR kits
      • Probe-based qPCR kits
      • TB Green-based qPCR kits
      • Videos: qPCR analysis for challenging inputs
      • qRT-PCR to detect RNA present at low levels
    • Technical notes
      • Unbiased preamplification of limited samples
      • Accurate gene expression analysis with TB Green Premix Ex Taq II
      • Efficient quantification of human gene expression with PrimeScript Reverse Transcriptase
      • Rapid qPCR analysis from blood samples using PrimeScript Reverse Transcriptase
      • Monitoring siRNA knockdown
      • qPCR without optimization using TB Green Premix Ex Taq
      • Fast synthesis of cDNA templates for real-time RT-PCR
      • Specific, consistent real-time PCR with TB Green Premix Ex Taq II
    • FAQs
  • cDNA synthesis
    • Premium total and poly A+ RNA
    • SMARTer RACE 5'/3' Kit—advances in SMARTer PCR cDNA synthesis
    • Cloning antibody variable regions
New products
Need help?
Contact Sales
Save on In-Fusion Cloning starter kits 50% off In-Fusion Cloning starter kits!
In-Fusion Cloning kits In-Fusion Cloning kits
Download the In-Fusion cloning infographic Download our infographic about seamless cloning
In-Fusion Cloning Interest Sign up for In-Fusion updates and promotions

Cloning learning center

In-Fusion Cloning

In-Fusion Cloning is a ligation-free technology which accommodates a wide range of applications, including single- and multiple-insert cloning, high-throughput cloning, and site-directed mutagenesis. With streamlined protocols, fast reaction times, and high accuracy, these kits minimize your experimental workload.

By leveraging the deep enzymology expertise of Takara Bio, we also offer reagents for all of your traditional cloning needs, including ligation kits, restriction enzymes, and modifying enzymes.

Find the right In-Fusion Cloning kit for your needs »


High-throughput cloning

Rapid, efficient antibody development

Save time with a streamlined, highly accurate cloning method adaptable to high-throughput applications.

High-throughput In-Fusion Cloning kits

High-throughput formats for projects that require a large number of clones, e.g., expression screening studies & library construction.


Seamless cloning tips, tricks, and troubleshooting

In‑Fusion Cloning FAQs

Learn more about In‑Fusion Cloning, including applications, tips, primer design, and vector and insert requirements.

In‑Fusion Cloning tips

Explore new and interesting tips and solutions for seamless PCR cloning with In-Fusion technology.

Seamless cloning primer design

Useful tips to keep in mind when designing your seamless cloning projects.

Seamless cloning methods

Learn about Gibson Assembly, Type IIS restriction enzymes, and In-Fusion Cloning.

Improve seamless cloning accuracy

View data on how different seamless cloning mechanisms yield different results in cloning accuracy.


In-Fusion Cloning online tools

Primer design tool

Design your In-Fusion primers with our step-by-step design tool, or access the molar ratio calculator and construct simulator.

Construct simulation software

SnapGene software allows easy planning and visualization of molecular biology procedures, including In-Fusion Cloning.

Molar ratio calculator

Calculate the optimal amounts of vector and insert for an In-Fusion Cloning reaction. Just enter the sizes of both vector and insert(s), in base pairs.


Cloning kit formats

In-Fusion Cloning selection guide

Select the right In-Fusion Cloning kit for fast, efficient, and accurate ligation-free cloning for your application.

High-throughput In-Fusion Cloning kits

High-throughput formats for projects that require a large number of clones, e.g., expression screening studies & library construction.

Ligation kit selection guide

Choose the best DNA ligation kit for your experiment, based on speed, insert size, and more.


In-Fusion Cloning applications

In-Fusion Cloning tech notes

View application data on using In-Fusion for all of your cloning needs.

In-Fusion Cloning citations

Read a wide range of In-Fusion Cloning citations, organized by application.

Mutagenesis with In-Fusion Cloning

A single, flexible system for deletions, base substitutions, or additions.

Efficient multiple-fragment cloning

With a newly optimized protocol, In‑Fusion Cloning ensures a high level of accuracy for single- and multiple-insert cloning.

Sign up to stay updated

Be the first to learn new information about In-Fusion Cloning as soon as it's available.


Traditional cloning resources

Ligation cloning overview

Ligation kits for your traditional cloning projects. Choose based on speed, insert length, blunt vs A-overhang vs sticky ends, and other criteria.

Ligation product guide

Choose the best DNA ligation kit for your experiment, based on speed, insert size, and more.

Restriction enzyme overview

Learn more about restriction enzymes: instructions for use, star activity, buffer activity, and more.


A simple approach to flu vaccine research

Constant genetic variation is the norm for influenza, making vaccine research challenging. Instead of designing primers for each of the eight influenza RNA segments, a clever team of researchers identified a single primer pair, then relied on In-Fusion Cloning for accurate, fast, and standardized subcloning. Learning how to cut the time from outbreak to vaccine: That's Good Science!

Learn more about In-Fusion Cloning

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
  • Corporate development
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2019 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • Automation systems
  • SmartChip Real-Time PCR System, chips, and reagents
  • Apollo system
  • ICELL8 system and software
  • Next-generation sequencing
  • Single-cell RNA- and DNA-seq
  • RNA-seq
  • DNA-seq
  • Immune profiling
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Human iPS cell gene editing systems
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • cDNA synthesis
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion Cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Cell biology assays
  • Extracellular vesicle miniprep
  • Exosome isolation (cell culture)
  • Reporter systems
  • Apoptosis detection kits
  • Epigenetics
  • Signal transduction
  • Gene function
  • Gene editing
  • Fluorescent proteins
  • Viral transduction
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Fluorescent protein plasmids
  • Capturem his-tagged purification sale
  • In-Fusion Cloning sale
  • qPCR promotion
  • Nucleic acid purification sale
  • Nucleic acid purification kit samples
  • Power medium promotion
  • CRISPR-Cas9 promotion
  • Lenti-X special offers
  • PCR samples
Alzheimer's disease research

Alzheimer's disease research

Alzheimer's disease (AD) affects ~5.8 million adults and is the sixth leading cause of death in the US alone. Over 400 AD clinical trials are currently under way, investigating the possibilities of immunotherapy, our microbiome, and inflammation as prospective therapeutic targets. We have a variety of best-in-class products that can help your AD research move forward.

See how our best-in-class solutions can help to advance your AD research

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • Apollo library prep system introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Selection guide
  • Product line overview
  • Technical notes
  • FAQs and tips
  • Webinars
  • Posters
  • Real-time PCR
  • Product finder
  • Reaction size guidelines for qPCR
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Overview
  • Technical notes
  • FAQs
  • Stem cell research
  • Applications
  • Technical notes
  • Webinars
  • Videos
  • Protocols
  • Citations
  • Nucleic acid purification
  • Product finder
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • Automated DNA and RNA purification
  • RNA purification
  • Hard-to-lyse samples
  • cDNA synthesis
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • PCR FAQs
  • Cloning
  • In-Fusion Cloning tools
  • In-Fusion Cloning guide
  • In‑Fusion Cloning FAQs
  • In‑Fusion Cloning tips
  • Choosing a seamless cloning method
  • Seamless cloning primer design
  • Cell biology assays
  • Extracellular vesicle isolation from biofluids
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Protein research
  • Capturem rapid purification technology
  • Antibody purification
  • His-tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Antibodies and ELISA
Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Website FAQs
  • Feedback
  • Trademarks
  • License statements
  • Vector information
  • Vector document overview
  • Vector document finder
  • Online tools
  • Business development
  • Partnering & OEM solutions
  • In licensing
  • Out licensing
  • Submit a licensing request
  • Instrument & reagent services
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

Learn more

  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • Announcements
  • Events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Quality statement
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Trademarks
  • License statements
  • That's Good Science!
  • Season one
  • Season two
  • Season three
  • BioView blog
  • That's Good Support!
  • Automation
  • Cancer research
  • Career spotlights
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • About our blog
  • Need help?
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
Best-in-class products, expert support, superior value

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

A simple approach to flu vaccine research

Constant genetic variation is the norm for influenza, making vaccine research challenging. Instead of designing primers for each of the eight influenza RNA segments, a clever team of researchers identified a single primer pair, then relied on In-Fusion Cloning for accurate, fast, and standardized subcloning. Learning how to cut the time from outbreak to vaccine: That's Good Science!

Learn more about In-Fusion Cloning

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • Feedback
  • About
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System, chips, and reagents
  • Apollo system
  • ICELL8 system and software
  • Next-generation sequencing
  • Single-cell RNA- and DNA-seq
  • RNA-seq
  • DNA-seq
  • Immune profiling
  • Epigenetics and small RNA sequencing
  • NGS accessories
  • Gene function
  • Gene editing
  • Fluorescent proteins
  • Viral transduction
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • ProteoTuner protein control systems
  • iDimerize inducible protein interaction systems
  • Transfection reagents
  • Mammalian expression plasmids
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Human iPS cell gene editing systems
  • Accessories
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Expression vectors & systems
  • Glycobiology
  • Antibodies and immunoprecipitation
  • SDS-PAGE & western blotting
  • Protein sequencing
  • Accessory enzymes
  • PCR
  • Most popular polymerases
  • Standard PCR
  • High-yield PCR
  • High-fidelity PCR
  • Fast PCR
  • Long-range PCR
  • GC rich PCR
  • Direct PCR
  • PCR master mixes
  • Custom business friendly and automation-ready solutions
  • Molecular diagnostic products
  • GMP-grade products
  • Application-specific PCR
  • Other PCR-related products
  • PCR thermal cyclers
  • Cloning
  • In-Fusion Cloning
  • Competent cells
  • Ligation kits
  • Mutagenesis kits
  • Ligation enzymes
  • Restriction enzymes
  • Modifying enzymes
  • Legacy cloning products
  • X-Gal and IPTG
  • Linkers, primers, and cloning vectors
  • Agarose gel electrophoresis
  • Nucleic acid extraction
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • RNA cleanup kits
  • Viral DNA and RNA purification kits
  • Accessories and components
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Secondary antibodies
  • Antibody and ELISA accessories
  • Fluorescent protein antibodies
  • Cell biology assays
  • Extracellular vesicle miniprep
  • Exosome isolation (cell culture)
  • Reporter systems
  • Apoptosis detection kits
  • Epigenetics
  • Cell biology reagents
  • RNA interference
  • Cell-culture accessories
  • Signal transduction
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • Real-time PCR primer sets
  • References and standards for qPCR
  • RNA extraction and analysis for real-time qPCR
  • Application-specific qPCR
  • cDNA synthesis
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • cDNA synthesis accessories
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • Apollo library prep system introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Selection guide
  • Product line overview
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Webinars
  • Citations
  • Posters
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Stem cell research
  • Applications
  • Technical notes
  • Posters
  • Webinars
  • Videos
  • Protocols
  • FAQs
  • Citations
  • Selection guides
  • Overview
  • Protein research
  • Capturem rapid purification technology
  • Antibody purification
  • His-tag purification
  • Other tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Expression systems
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • PCR FAQs
  • Go green with lyophilized enzymes
  • LA PCR technology
  • Cloning
  • In-Fusion Cloning tools
  • In-Fusion Cloning guide
  • In‑Fusion Cloning FAQs
  • In‑Fusion Cloning tips
  • Choosing a seamless cloning method
  • Seamless cloning primer design
  • In-Fusion Cloning applications collection
  • Efficient cloning for sgRNA/Cas9 plasmids
  • In-Fusion Cloning tech notes
  • In-Fusion Cloning citations
  • EcoDry reagents and sustainability
  • Mutagenesis with In-Fusion Cloning
  • Efficient multiple-fragment cloning
  • Sign up to stay updated
  • Traditional molecular cloning
  • Nucleic acid purification
  • Product finder
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • Parallel DNA, RNA & protein
  • Automated DNA and RNA purification
  • RNA purification
  • Hard-to-lyse samples
  • Antibodies and ELISA
  • Osteocalcin focus
  • Cell biology assays
  • Extracellular vesicle isolation from biofluids
  • Cell viability kits
  • Exosome isolation from cell culture
  • Mir-X microRNA quantification
  • Real-time PCR
  • Product finder
  • Reaction size guidelines for qPCR
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Overview
  • Technical notes
  • FAQs
  • cDNA synthesis
  • Premium total and poly A+ RNA
  • SMARTer RACE 5'/3' Kit—advances in SMARTer PCR cDNA synthesis
  • Cloning antibody variable regions
  • Services & Support
  • Instrument services
  • OEM & custom enzyme manufacturing
  • Cell and gene therapy manufacturing services
  • Stem cell services
  • Customer service
  • Technical support
  • Shipping & delivery
  • Sales
  • Website FAQs
  • Corporate development
  • Vector information
  • Online tools
  • Instrument & reagent services
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • Cell and gene therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Stem cell services
  • Clinical-grade hES cell line derivation
  • Sourcing
  • Reprogramming
  • Cell banking
  • Gene editing
  • Directed differentiation to hepatocytes
  • Directed differentiation to beta cells
  • Directed differentiation to definitive endoderm cells
  • Human pathogen and sterility testing
  • Corporate development
  • Partnering & OEM solutions
  • In licensing
  • Out licensing
  • Submit a licensing request
  • Vector information
  • Vector document overview
  • Vector document finder
  • Areas of interest
  • Cancer research
  • Immunotherapy research
  • Alzheimer's disease research
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • About
  • Manufacturing DSS Takara Bio India
  • Careers
  • Quality and compliance
  • Need help?
  • Our brands
  • Our history
  • Trademarks
  • License statements
  • Announcements
  • Our partners
  • BioView blog
  • Takara Bio affiliates & distributors
  • That's Good Science!
  • Special offers
  • New products
  • Events
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • BioView blog
  • That's Good Support!
  • Automation
  • Cancer research
  • Career spotlights
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • About our blog
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • That's Good Science!
  • Season one
  • Season two
  • Season three
  • Special offers
  • Buy Again and Save More on DSS Takara Bio India Manufactured products
  • Classic Campaign on Takara Bio and Macherey Nagel products
  • Fluorescent protein plasmids
  • Fluorescent protein plasmids
  • Capturem his-tagged purification sale
  • In-Fusion Cloning sale
  • qPCR promotion
  • Nucleic acid purification sale
  • Nucleic acid purification kit samples
  • Power medium promotion
  • CRISPR-Cas9 promotion
  • Lenti-X special offers
  • PCR samples
  • EOY Promo (GBP)
  • qPCR-RT promo (DKK)
  • RT-qPCR bundle promotion (EUR)
  • RT-qPCR bundle promotion (SEK)
  • RT-qPCR bundle promotion (CHF)
  • RT-qPCR bundle promotion (GBP)
  • Events
  • Calendar
  • Conferences
  • Speak with us
  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About