In-Fusion Cloning mechanism
Clone any insert, with any vector, at any site. In-Fusion seamless cloning technology makes it easy! Here’s how.
Learning centers
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Automation systems
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SmartChip Real-Time PCR System introduction
- High-throughput detection of SARS-CoV-2
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- SmartChip Real-Time PCR System technical specifications
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ICELL8 introduction
- ICELL8 technology overview
- ICELL8 cx technical specifications
- ICELL8 technical specifications (original system)
- ICELL8 system vs plate-seq
- Webinars
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Technical notes
- Enhancing biomarker discovery with SMART-Seq Pro kit and ICELL8 cx system
- ICELL8 cx system target enrichment for fusions
- ICELL8 cx system reagent formulation and dispense guidelines
- Improved detection of gene fusions, SNPs, and alternative splicing
- Full-length transcriptome analysis
- High-throughput single-cell ATAC-seq
- Protocol: High-throughput single-cell ATAC-Seq
- Single-cell identification with CellSelect Software
- Single-cell analysis elucidates cardiomyocyte differentiation from iPSCs
- Combined TCR profiling and 5’ DE in single cells
- Automated, high-throughput TCR profiling
- Sample preparation protocols
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- Apollo library prep system introduction
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SmartChip Real-Time PCR System introduction
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Next-generation sequencing
- Product line overview
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Technical notes
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DNA-seq
- High-resolution CNV detection using PicoPLEX Gold DNA-Seq
- Next-gen WGA method for CNV and SNV detection from single cells
- Low-volume DNA shearing for ThruPLEX library prep
- Low-input whole-exome sequencing
- Cell-free nucleic acid sequencing
- DNA-seq from FFPE samples
- ThruPLEX HV data sheet
- Improvements to ThruPLEX HV
- ThruPLEX HV outperforms NEBNext Ultra II
- Comparing ThruPLEX HV PLUS to Kapa and NEBNext
- Low cell number ChIP-seq using ThruPLEX DNA-Seq
- Accurate detection of low-frequency variants using molecular tags
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Immune Profiling
- TCRv2 kit validated for rhesus macaque samples
- TCR repertoire profiling from mouse samples (bulk)
- BCR repertoire profiling from mouse samples (bulk)
- Improved TCR repertoire profiling from human samples (bulk)
- TCR repertoire profiling from human samples (single cells)
- BCR repertoire profiling from human samples (bulk)
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RNA-seq
- All-in-one cDNA synthesis and library prep from single cells
- Automation-friendly, all-in-one cDNA synthesis and library prep
- All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
- 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
- Full-length mRNA-seq for target capture
- Stranded libraries from single cells
- Stranded libraries from picogram-input total RNA (v3)
- Stranded libraries from 100 pg-100 ng total RNA
- Stranded libraries from 100 ng - 1 ug total RNA
- Stranded libraries from FFPE inputs (v2)
- Nonstranded libraries from FFPE inputs
- Cogent NGS Analysis Pipeline
- Cogent NGS Discovery Software
- SMART-Seq DE3 Demultiplexer
- Epigenetic sequencing
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DNA-seq
- Featured kits
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DNA-seq protocols
- Using UMIs with ThruPLEX Tag-Seq HV
- Exome capture with Illumina Nextera Rapid Capture
- Targeted capture with Roche NimbleGen SeqCap EZ
- Targeted capture with IDT xGen panels
- Targeted capture with Agilent SureSelectXT
- Targeted capture with Agilent SureSelectXT2
- Targeted capture with Agilent SureSelectQXT
- Plasma preparation for ThruPLEX Plasma-Seq workflow
- Bioinformatics resources
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Webinars
- Harnessing the power of full-length transcriptome analysis for biomarker discoveries
- SMART-Seq Pro kits for biomarker detection
- Takara Bio Single-Cell Workshop, Spring 2021
- Capturing biological complexity by high-resolution single-cell genomics
- Sensitivity and scale for neuron multiomics
- Taking single-cell RNA-seq by STORM
- Pushing the limits of sensitivity for single-cell applications
- Liver metabolic function, dissecting one cell at a time
- Single-Cell Workshop at 2020 NextGen Omics Series UK
- TCR-seq methods: when to use which
- Immunogenomics to accelerate immunotherapy
- Total RNA sequencing of liquid biopsies
- MeD-Seq, a novel method to detect DNA methylation
- Automating full-length single-cell RNA-seq libraries
- Single-cell DNA-seq
- Single-cell whole transcriptome analysis
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Gene function
- Gene editing
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Viral transduction
- Recombinant virus comparison
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Lentivirus
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- Lenti-X packaging
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- Retrovirus
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- Inducible systems
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Transfection reagents
- Xfect Transfection Reagent
- Transfection tips
- Cell lines transfected with the Xfect reagent
- Protein transfection FAQs
- Xfect RNA Transfection Reagent
- Transfecting with single shots video
- Single shots picture protocol
- Transfection reagent products
- Protocol: transfection of cerebellar slice cultures with Xfect reagent
- Calcium phosphate transfection of neurons
- Fluorescent proteins
- Cell biology assays
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Stem cell research
- Overview
- Protocols
- Applications
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Technical notes
- Pluripotent stem cells
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Gene editing in hiPS cells
- Tagging an endogenous gene with AcGFP1 in hiPS cells
- Tagging an endogenous gene with a myc tag in hiPS cells
- Generating clonal hiPS cell lines deficient in CD81
- Introducing a tyrosinemia-related SNP in hiPS cells
- Inserting an expression cassette into the AAVS1 locus in hiPS cells
- Editing hiPS cells using electroporation
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- Single-cell cloning of hiPS cells
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Citations
- Cellartis MSC Xeno-Free Culture Medium
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- Cellartis hES-MP 002.5
- Cellartis hPS cell-derived cardiomyocytes
- Cellartis iPS Cell to Hepatocyte Differentiation System
- GS1-R
- 2i mES/iPSC medium
- iMatrix-511
- 3i mES/iPSC medium
- NDiff 227
- NDiff N2
- RHB-A
- STEM101
- STEM121
- STEM123
- Selection guides
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Protein research
- Capturem technology
- Antibody immunoprecipitation
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His-tag purification
- Purification methods overview
- TALON resin selection guide
- Selection guide: His60 resin
- xTractor Buffer is optimized for superior protein yield
- Why tag a protein?
- Tech note: cobalt resin
- Simplified purification of active, secreted his-tagged proteins
- Overview: His60
- Tech note: Capturem technology
- Tech note: Capturem large volume
- Magnetic beads
- FAQs: TALON
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Protocols
- Video: Capturem his maxiprep
- Video: Capturem his miniprep
- Visual protocol: Capturem his maxiprep
- Visual protocol: Capturem his miniprep
- Capturem nickel column reagent compatibility
- TALON reagent compatibility
- His60 reagent compatibility
- TALON: Native vs denaturing purification
- Protocol: denaturing purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, pH elution
- Other tag purification
- Phosphoprotein and glycoprotein purification
- Matchmaker Gold yeast two-hybrid systems
- Expression systems
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PCR
- Citations
- Selection guides
- PCR enzyme brochure
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Technical notes
- Rapid, high-performance multiplex PCR
- EmeraldAmp outperforms MyTaq Red mix
- Fast and accurate PCR
- Methylation studies
- Hot-start PCR
- Long-range PCR with LA Taq
- Direct PCR from human nail
- Direct PCR from meat samples
- Megaprimer PCR with PrimeSTAR GXL
- Amplifying GC-rich templates
- Titanium Taq for high-throughput genotyping
- Colony PCR in under an hour
- High-throughput endpoint PCR
- Direct PCR from blood
- PrimeSTAR GXL for targeted sequencing
- Detecting somatic mosaicism using massively parallel sequencing
- FAQ
- Go green with lyophilized enzymes
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Cloning
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In-Fusion Cloning general information
- In-Fusion Cloning overview
- In-Fusion Cloning guide
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In-Fusion Cloning and competition
- In-Fusion Snap Assembly vs. GeneArt Gibson Assembly HiFi
- In-Fusion Snap Assembly vs. NEBuilder HiFi
- Sequence accuracy in seamless cloning
- Choosing a seamless cloning method
- Improving background over Gibson Assembly
- A successful alternative to ligation cloning
- Single- and multiple-insert cloning
- Easy cloning into lentiviral vectors
- Outperforming TOPO cloning
- In-Fusion Cloning citations
- Stellar Competent Cells product overview and performance data
- EcoDry reagents and sustainability
- Primer design and other tools
- In‑Fusion Cloning tips and FAQs
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Applications and technical notes
- In-Fusion Cloning applications collection
- Efficient multiple-fragment cloning
- Mutagenesis with In-Fusion Cloning
- Rapid, high-throughput cloning for antibody development
- Solve a synthesis challenge with easy multiple-insert cloning
- Direct cloning into large vectors
- Simplified insertion of a GFP-encoding cassette into a 100-kb plasmid
- Efficient cloning for sgRNA/Cas9 plasmids
- In-Fusion Cloning of sgRNAs
- De novo insertion of small fusion protein tags
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Traditional molecular cloning
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Restriction enzyme overview
- General information about restriction enzymes
- Star activity of restriction enzymes
- Inactivation of restriction enzymes
- Buffer activity with restriction enzymes
- Universal buffers for double digestion with restriction enzymes
- Restriction enzymes affected by methylation
- Methylation-sensitive restriction enzymes
- QC of restriction enzymes
- Ligation cloning overview
- Ligation product guide
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Restriction enzyme overview
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In-Fusion Cloning general information
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Nucleic acid purification
- Nucleic acid extraction webinars
- Product demonstration videos
- Product finder
- Plasmid kit selection guide
- Plasmid purification
- Genomic DNA purification
- DNA/RNA cleanup and extraction
- RNA purification
- RNA purification kit finder
- Viral DNA and RNA purification
- Parallel DNA, RNA & protein
- Automated DNA and RNA purification
- Accessory selection guides
- Microbiome
- Antibodies and ELISA
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Real-time PCR
- Overview
- Product finder
- Reaction size guidelines
- Real-time PCR products brochure
- Real-time PCR tutorial videos
- Guest webinar: extraction-free SARS-CoV-2 detection
- Guest webinar: developing and validating molecular diagnostic tests
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Technical notes
- Unbiased preamplification of limited samples
- Accurate gene expression analysis with TB Green Premix Ex Taq II
- Efficient quantification of human gene expression with PrimeScript Reverse Transcriptase
- Rapid qPCR analysis from blood samples using PrimeScript Reverse Transcriptase
- Monitoring siRNA knockdown
- qPCR without optimization using TB Green Premix Ex Taq
- Fast synthesis of cDNA templates for real-time RT-PCR
- Specific, consistent real-time PCR with TB Green Premix Ex Taq II
- Mir-X microRNA quantification
- FAQs
- cDNA synthesis
Cloning learning center
Cloning is simple and elegant with In-Fusion seamless cloning technology. Its ligation-free protocol accommodates a wide range of applications, including single- and multiple-insert cloning, high-throughput cloning, and site-directed mutagenesis. With streamlined protocols, fast reaction times, and high accuracy, these kits minimize your experimental workload.
In-Fusion Cloning primer design tool | Traditional molecular cloning | Product information
In-Fusion Cloning mechanism
Clone any insert, with any vector, at any site. In-Fusion seamless cloning technology makes it easy! Here’s how.
Applications using In-Fusion Cloning
In-Fusion Cloning is beautifully versatile—learn about the different ways you can put it to work.
In-Fusion Cloning vs. other seamless cloning technologies
In-Fusion Cloning is the most elegant and simple seamless cloning technology available. Learn why.
In-Fusion Cloning: general information
Find information about In-Fusion technology, product guides, and performance vs. the competition.
Primer design and other tools
Design your In-Fusion primers with our step-by-step design tool, or access the molar ratio calculator and construct simulator.
In‑Fusion Cloning tips and FAQs
Learn more about In‑Fusion Cloning, including applications, tips, primer design, and vector and insert requirements.
Applications and technical notes
View application data on how In-Fusion technology performs for all of your cloning needs.
Sign up to stay updated
Be the first to learn new information about In-Fusion Cloning as soon as it's available.
Traditional molecular cloning
Choose from a variety of high-quality reagents and kits for every step of your molecular cloning workflow.
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