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Cloning learning center

In-Fusion Cloning

In-Fusion Cloning is a ligation-free technology which accommodates a wide range of applications, including single- and multiple-insert cloning, high-throughput cloning, and site-directed mutagenesis. With streamlined protocols, fast reaction times, and high accuracy, these kits minimize your experimental workload.

By leveraging the deep enzymology expertise of Takara Bio, we also offer reagents for all of your traditional cloning needs, including ligation kits, restriction enzymes, and modifying enzymes.

Seamless cloning tips, tricks, and troubleshooting

In‑Fusion Cloning FAQs

Learn more about In‑Fusion Cloning, including applications, tips, primer design, and vector and insert requirements.

In‑Fusion Cloning tips

Explore new and interesting tips and solutions for seamless PCR cloning with In-Fusion technology.

Seamless cloning primer design

Useful tips to keep in mind when designing your seamless cloning projects.

In-Fusion Cloning online tools

Primer design tool

Design your In-Fusion primers with our step-by-step design tool, or access the molar ratio calculator and construct simulator.

Construct simulation software

SnapGene software allows easy planning and visualization of molecular biology procedures, including In-Fusion Cloning.

Molar ratio calculator

Calculate the optimal amounts of vector and insert for an In-Fusion Cloning reaction. Just enter the sizes of both vector and insert(s), in base pairs.

Cloning kit selection guides

In-Fusion Cloning selection guide

Select the right In-Fusion Cloning kit for fast, efficient, and accurate ligation-free cloning for your application.

Ligation kit selection guide

Choose the best DNA ligation kit for your experiment, based on speed, insert size, and more.

In-Fusion Cloning applications

In-Fusion Cloning tech notes

View application data on using In-Fusion for all of your cloning needs.

In-Fusion Cloning webinars

Watch our series of In-Fusion Cloning webinars to learn how to simplify your cloning project.

In-Fusion Cloning citations

Read a wide range of In-Fusion Cloning citations, organized by application.

Mutagenesis with In-Fusion Cloning

A single, flexible system for deletions, base substitutions, or deletions.

Efficient multiple-fragment cloning

With a newly optimized protocol, In‑Fusion Cloning ensures a high level of accuracy for single- and multiple-insert cloning.

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Traditional cloning resources

Ligation cloning overview

Ligation kits for your traditional cloning projects. Choose based on speed, insert length, blunt vs A-overhang vs sticky ends, and other criteria.

Ligation product guide

Choose the best DNA ligation kit for your experiment, based on speed, insert size, and more.

Restriction enzyme overview

Learn more about restriction enzymes: instructions for use, star activity, buffer activity, and more.

1.12 | Simplicity conquers complexity in influenza research

With constant genetic variation being the norm for influenza, researchers need elegant solutions to build effective vaccine defenses. Instead of designing primers for each of the eight influenza RNA segments, Bin Zhou and colleagues identified a single primer pair to amplify all subtypes and species, then relied on In-Fusion PCR cloning kits for an accurate, fast, and standardized subcloning method. Learning how to cut the time from outbreak to vaccine: That's Good Science!

Learn more about In-Fusion Cloning

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