In-Fusion Cloning mechanism
Clone any insert, with any vector, at any site. In-Fusion seamless cloning technology makes it easy! Here’s how.
Learning centers
-
Automation systems
-
SmartChip Real-Time PCR System introduction
- SmartChip Real-Time PCR System overview
- SmartChip Real-Time PCR System technical specifications
- SmartChip Real-Time PCR System applications
- SmartChip Real-Time PCR System video resources
- Request a SmartChip system demo
- Apollo library prep system introduction
-
ICELL8 introduction
- ICELL8 technology overview
- ICELL8 cx technical specifications
- ICELL8 technical specifications (original system)
-
Technical notes
- Protocol: Oxford Nanopore library prep and quantification
- ICELL8 cx system target enrichment for fusions
- ICELL8 cx system reagent formulation and dispense guidelines
- Protocol: High-throughput single cell DNA-seq
- Improved detection of gene fusions, SNPs, and alternative splicing
- Full-length transcriptome analysis
- Protocol: High-throughput single-cell ATAC-Seq
- High-throughput single-cell ATAC-seq
- Single-cell identification with CellSelect Software
- Single-cell analysis elucidates cardiomyocyte differentiation from iPSCs
- Combined TCR profiling and 5’ DE in single cells
- Automated, high-throughput TCR profiling
- Sample preparation protocols
- Video resources
- Sign up: cardiomyocyte webinar
- Posters
- Citations
- System & software notices
-
SmartChip Real-Time PCR System introduction
-
Next-generation sequencing
- Product line overview
-
Technical notes
-
Single-cell RNA- and DNA-seq
- All-in-one cDNA synthesis and library prep from single cells
- Highest sensitivity for single-cell mRNA-seq
- Stranded libraries from single cells
- Streamlined single-cell mRNA-seq
- Full-length mRNA libraries from single cells (SMART-Seq v4)
- 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
- Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
- Full-length single-cell library method comparison
- High-resolution CNV detection using PicoPLEX Gold DNA-Seq
- Next-gen WGA method for CNV and SNV detection from single cells
-
RNA-seq
- Automation-friendly, all-in-one cDNA synthesis and library prep
- All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
- Stranded libraries from picogram-input total RNA (v2)
- Stranded libraries from FFPE inputs (v2)
- Stranded libraries from 100 ng - 1 ug total RNA
- Stranded libraries from 100 pg-100 ng total RNA
- Stranded libraries from picogram-input total RNA (v1)
- Stranded RNA-seq competitor kit comparison
- Nonstranded libraries from FFPE inputs
- Sensitive capture of full-length transcript information with targeted RNA-seq
-
DNA-seq
- ThruPLEX HV outperforms NEBNext Ultra II
- Accurate detection of low-frequency variants using molecular tags
- Streamlined DNA-seq from challenging samples
- Low cell number ChIP-seq using SMARTer ThruPLEX
- Cell-free DNA sequencing
- Sequencing analysis of low-frequency mutations in cfDNA
- DNA-seq from FFPE samples
- Low-input whole-exome sequencing
- Tag-seq variant detection
- Low-volume DNA shearing for SMARTer ThruPLEX library prep
-
Immune profiling
- Improved TCR repertoire profiling from human samples (bulk)
- TCR repertoire profiling from human samples (single cells)
- TCR repertoire profiling from human samples (bulk)
- BCR repertoire profiling from human samples (bulk)
- TCR repertoire profiling from mouse samples (bulk)
- BCR repertoire profiling from mouse samples (bulk)
- Epigenetics and smRNA-seq
-
Single-cell RNA- and DNA-seq
- Featured kits
- Technology and application overviews
- FAQs and tips
-
DNA-seq protocols
- Using UMIs with ThruPLEX Tag-Seq HV
- Exome capture with Illumina Nextera Rapid Capture
- Targeted capture with Roche NimbleGen SeqCap EZ
- Targeted capture with IDT xGen panels
- Targeted capture with Agilent SureSelectXT
- Targeted capture with Agilent SureSelectXT2
- Targeted capture with Agilent SureSelectQXT
- Plasma preparation for ThruPLEX Plasma-Seq workflow
-
Webinars
- Sign up: Total RNA sequencing of liquid biopsies
- Sign-up: MeD-Seq, a novel method to detect DNA methylation
- Sign up: automating full-length single-cell RNA-seq libraries
- Sign up: single-cell DNA-seq webinar
- Sign up: single-cell whole transcriptome analysis
- Single-cell RNA-seq
- Total RNA-seq
- Immune profiling
- Epigenetics
- Targeted RNA-seq
- DNA-seq
- Citations
- Posters
-
Gene function
- Gene editing
-
Viral transduction
- Recombinant virus comparison
- Product finder
- Transduction posters
-
Lentivirus
- Lenti-X FAQs
- Lentiviral workflow
- Lentiviral products guide
- Lentivirus biosafety
- Lentiviral tips
- Lentiviral vectors
- Lenti-X packaging
- Lenti-X Concentrator
- Lentiviral titration
- Lenti-X GoStix Plus video protocols
- Lenti-X GoStix Plus FAQs
- Rapid lentivirus titration by p24 ELISA
- Lentiviral particles
- Lentiviral particles—fluorescent
- Lentiviral particles webinar
- Lentiviral particles FAQs
- Retrovirus
- Adeno-associated virus
- Adenovirus
- T-cell transduction and culture
- Inducible systems
-
Transfection reagents
- Xfect Transfection Reagent
- Transfection tips
- Cell lines transfected with the Xfect reagent
- Protein transfection FAQs
- Xfect RNA Transfection Reagent
- Transfecting with single shots video
- Single shots picture protocol
- Transfection reagent products
- Protocol: transfection of cerebellar slice cultures with Xfect reagent
- Calcium phosphate transfection of neurons
- Fluorescent proteins
-
Stem cell research
- Applications
-
Technical notes
- Pluripotent stem cells
-
Gene editing in hiPS cells
- Tagging an endogenous gene with AcGFP1 in hiPS cells
- Tagging an endogenous gene with a myc tag in hiPS cells
- Generating clonal hiPS cell lines deficient in CD81
- Introducing a tyrosinemia-related SNP in hiPS cells
- Inserting an expression cassette into the AAVS1 locus in hiPS cells
- Editing hiPS cells using electroporation
- Editing hiPS cells using gesicle technology
- Single-cell cloning of hiPS cells
- Beta cells
- Hepatocytes
- Cardiomyocytes
- Neural stem cells
- Posters
- Webinars
- Videos
-
Protocols
- Getting started with hepatocyte differentiation
- Cardiomyocytes in FLIPR 384-well plate format
- Cardiomyocytes on the Patchliner system
- Cardiomyocytes on the Maestro MEA system
- Cardiomyocytes on the MED64 MEA system
- Cardiomyocytes on the CardioExcyte 96 system
- Cardiomyocytes on the xCELLigence RTCA CardioECR system
- Reprogramming fibroblasts
- Reprogramming PBMCs
- Spin embryoid body formation
- Transferring iPSCs from other media to DEF-CS
- Transferring iPSCs on MEFs to DEF-CS
- FAQs
-
Citations
- Cellartis Power Primary HEP Medium
- Cellartis DEF-CS 500 Culture System
- Cellartis Enhanced hiPS-HEP cells
- Cellartis hES-MP 002.5
- Cellartis hPS cell-derived cardiomyocytes
- Cellartis iPS Cell to Hepatocyte Differentiation System
- GS1-R
- 2i mES/iPSC medium
- iMatrix-511
- 3i mES/iPSC medium
- NDiff 227
- NDiff N2
- RHB-A
- STEM101
- STEM121
- STEM123
- Selection guides
- Overview
-
Protein research
- Capturem rapid purification technology
- Antibody purification
-
His-tag purification
- Why tag a protein?
- Tech note: cobalt resin
- Purification methods overview
- Simplified purification of active, secreted his-tagged proteins
- Overview: His60
- Tech note: Capturem technology
- Tech note: Capturem large volume
- Magnetic beads
- TALON resin selection guide
- Selection guide: His60 resin
- FAQs: TALON
-
Protocols
- Video: Capturem his maxiprep
- Video: Capturem his miniprep
- Visual protocol: Capturem his maxiprep
- Visual protocol: Capturem his miniprep
- Capturem nickel column reagent compatibility
- TALON reagent compatibility
- His60 reagent compatibility
- TALON: Native vs denaturing purification
- Protocol: denaturing purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, pH elution
- Other tag purification
- Phosphoprotein and glycoprotein purification
- Mass spectrometry digestion reagents
- Matchmaker Gold yeast two-hybrid systems
- Expression systems
-
PCR
- Citations
- Selection guides
- PCR enzyme brochure
-
Technical notes
- Rapid, high-performance multiplex PCR
- EmeraldAmp outperforms MyTaq Red mix
- Fast and accurate PCR
- Methylation studies
- Hot-start PCR
- Long-range PCR with LA Taq
- Direct PCR from human nail
- Direct PCR from meat samples
- Megaprimer PCR with PrimeSTAR GXL
- Amplifying GC-rich templates
- Titanium Taq for high-throughput genotyping
- Colony PCR in under an hour
- High-throughput endpoint PCR
- Direct PCR from blood
- PrimeSTAR GXL for targeted sequencing
- Detecting somatic mosaicism using massively parallel sequencing
- PCR FAQs
- Go green with lyophilized enzymes
-
Cloning
- In-Fusion Cloning overview
- Primer design and other tools
- In-Fusion Cloning guide
- In‑Fusion Cloning tips and FAQs
- Choosing a seamless cloning method
-
In-Fusion Cloning tech notes
- In-Fusion Cloning applications collection
- Efficient multiple-fragment cloning
- Mutagenesis with In-Fusion Cloning
- Simplified insertion of a GFP-encoding cassette into a 100-kb plasmid
- In-Fusion Cloning of sgRNAs
- Sequence accuracy in seamless cloning
- De novo insertion of small fusion protein tags
- Improving background over Gibson Assembly
- Rapid, high-throughput cloning for antibody development
- A successful alternative to ligation cloning
- Single- and multiple-insert cloning
- Direct cloning into large vectors
- Easy cloning into lentiviral vectors
- Outperforming TOPO cloning
- Solve a synthesis challenge with easy multiple-insert cloning
- Efficient cloning for sgRNA/Cas9 plasmids
- In-Fusion Cloning citations
- EcoDry reagents and sustainability
- Sign up to stay updated
-
Traditional molecular cloning
-
Restriction enzyme overview
- General information about restriction enzymes
- Star activity of restriction enzymes
- Inactivation of restriction enzymes
- Buffer activity with restriction enzymes
- Universal buffers for double digestion with restriction enzymes
- Restriction enzymes affected by methylation
- Methylation-sensitive restriction enzymes
- QC of restriction enzymes
- Ligation cloning overview
- Ligation product guide
-
Restriction enzyme overview
-
Nucleic acid purification
- Product finder
- Plasmid purification
-
Genomic DNA purification
- NucleoSpin Tissue
- NucleoSpin DNA RapidLyse
- NucleoSpin Tissue XS
- NucleoSnap cfDNA
- NucleoSpin cfDNA XS
- NucleoMag cfDNA
- NucleoMag Pathogen
- NucleoMag Virus
- NucleoMag Blood
- NucleoBond HMW DNA
- NucleoMag DNA FFPE
- NucleoSpin 96 DNA FFPE
- NucleoMag DNA Swab
- NucleoMag VET
- NucleoMag 384 Plant
- NucleoSpin cfDNA Midi
- DNA/RNA cleanup and extraction
- Parallel DNA, RNA & protein
- Automated DNA and RNA purification
- RNA purification
-
Hard-to-lyse samples
- NucleoMag DNA Bacteria
- NucleoSpin Plant II
- NucleoSpin RNA Plant and Fungi
- NucleoMag DNA Food
- NucleoSpin DNA Insect
- NucleoSpin Soil
- NucleoSpin 8/96 Soil
- NucleoBond RNA Soil
- NucleoSpin DNA Lipid Tissue
- NucleoSpin DNA Stool
- NucleoSpin RNA Stool
- NucleoSpin Microbial DNA
- NucleoMag DNA/RNA Water
- Sample homogenization beads
- Antibodies and ELISA
- Cell biology assays
-
Real-time PCR
- Product finder
- Reaction size guidelines for qPCR
- Real-time PCR products brochure
- Real-time PCR tutorial videos
- Overview
-
Technical notes
- Unbiased preamplification of limited samples
- Accurate gene expression analysis with TB Green Premix Ex Taq II
- Efficient quantification of human gene expression with PrimeScript Reverse Transcriptase
- Rapid qPCR analysis from blood samples using PrimeScript Reverse Transcriptase
- Monitoring siRNA knockdown
- qPCR without optimization using TB Green Premix Ex Taq
- Fast synthesis of cDNA templates for real-time RT-PCR
- Specific, consistent real-time PCR with TB Green Premix Ex Taq II
- FAQs
- cDNA synthesis
Cloning learning center
Cloning is simple and elegant with In-Fusion seamless cloning technology. Its ligation-free protocol accommodates a wide range of applications, including single- and multiple-insert cloning, high-throughput cloning, and site-directed mutagenesis. With streamlined protocols, fast reaction times, and high accuracy, these kits minimize your experimental workload.
In-Fusion Cloning primer design tool | Traditional molecular cloning | Product information
In-Fusion Cloning mechanism
Clone any insert, with any vector, at any site. In-Fusion seamless cloning technology makes it easy! Here’s how.
Applications using In-Fusion Cloning
In-Fusion Cloning is beautifully versatile—learn about the different ways you can put it to work.
In-Fusion Cloning vs. other seamless cloning technologies
In-Fusion Cloning is the most elegant and simple seamless cloning technology available. Learn why.
In-Fusion Cloning resources
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2020 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.