In-Fusion Cloning is a ligation-free technology which accommodates a wide range of applications, including single- and multiple-insert cloning, high-throughput cloning, and site-directed mutagenesis. With streamlined protocols, fast reaction times, and high accuracy, these kits minimize your experimental workload.
By leveraging the deep enzymology expertise of Takara Bio, we also offer reagents for all of your traditional cloning needs, including ligation kits, restriction enzymes, and modifying enzymes.
Save time with a streamlined, highly accurate cloning method adaptable to high-throughput applications.
High-throughput formats for projects that require a large number of clones, e.g., expression screening studies & library construction.
Learn more about In‑Fusion Cloning, including applications, tips, primer design, and vector and insert requirements.
Explore new and interesting tips and solutions for seamless PCR cloning with In-Fusion technology.
Useful tips to keep in mind when designing your seamless cloning projects.
Learn about Gibson Assembly, Type IIS restriction enzymes, and In-Fusion Cloning.
View data on how different seamless cloning mechanisms yield different results in cloning accuracy.
Design your In-Fusion primers with our step-by-step design tool, or access the molar ratio calculator and construct simulator.
SnapGene software allows easy planning and visualization of molecular biology procedures, including In-Fusion Cloning.
Calculate the optimal amounts of vector and insert for an In-Fusion Cloning reaction. Just enter the sizes of both vector and insert(s), in base pairs.
Select the right In-Fusion Cloning kit for fast, efficient, and accurate ligation-free cloning for your application.
High-throughput formats for projects that require a large number of clones, e.g., expression screening studies & library construction.
Choose the best DNA ligation kit for your experiment, based on speed, insert size, and more.
View application data on using In-Fusion for all of your cloning needs.
Read a wide range of In-Fusion Cloning citations, organized by application.
A single, flexible system for deletions, base substitutions, or additions.
With a newly optimized protocol, In‑Fusion Cloning ensures a high level of accuracy for single- and multiple-insert cloning.
Be the first to learn new information about In-Fusion Cloning as soon as it's available.
Ligation kits for your traditional cloning projects. Choose based on speed, insert length, blunt vs A-overhang vs sticky ends, and other criteria.
Choose the best DNA ligation kit for your experiment, based on speed, insert size, and more.
Learn more about restriction enzymes: instructions for use, star activity, buffer activity, and more.
Constant genetic variation is the norm for influenza, making vaccine research challenging. Instead of designing primers for each of the eight influenza RNA segments, a clever team of researchers identified a single primer pair, then relied on In-Fusion Cloning for accurate, fast, and standardized subcloning. Learning how to cut the time from outbreak to vaccine: That's Good Science!
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A simple approach to flu vaccine research
Constant genetic variation is the norm for influenza, making vaccine research challenging. Instead of designing primers for each of the eight influenza RNA segments, a clever team of researchers identified a single primer pair, then relied on In-Fusion Cloning for accurate, fast, and standardized subcloning. Learning how to cut the time from outbreak to vaccine: That's Good Science!
