Flexibility to isolate cells of any size is critical to single-cell analysis

Cell sizes are just as varied as the functions they perform. The MultiSample NanoDispenser (MSND) of the ICELL8 systems takes advantage of a large-bore nozzle for dispensing and Poisson distribution, allowing users to isolate cells of any size, including nuclei. The table below displays a few of the cell types that have been isolated using the system. For samples highlighted in blue, the ICELL8 Single-Cell System is the first to allow successful isolation of single cells, nuclei, or spheroids.

Visual depiction of sample sizes that can be isolated using the ICELL8 system. Samples can range from 5 µm up to 100 µm, including nuclei, B lymphocytes, neurons, and cardiomyocytes.

Cell line/type SpeciesSource
307 Mouse Pancreas
3T3 Mouse Fibroblast
A-20 Mouse B-lymphocyte
A-375 Human Melanoma
BaF/3 Mouse Pre-B cells
Beta-TC-6 Mouse Pancreas
CHO Hamster Ovary
Differentiated ESC Mouse Embryonic stem cells
Ear Mouse Inner ear organs
ESC Mouse Embryonic stem cells
FACS-sorted lymphocyte Human Bone marrow
Fetal neurons Human Fetal brain
Gut cells Mosquito Gut
H2452 Human Lung
HCT 116 Human Colon
HEK-293 Human Kidney embryo
HSPC Mouse Hematopoietic stem cells
K562 Human Bone marrow lymphoblast, CML
KU812 Human Blood, CML myeloblast
MCF7 Human Breast
MDA-MB-231 Human Mammary gland
MIA PaCa-2 Human Pancreas
Nasal epithelia Human Primary nasal scraping
NCH421K Human Glioma/glioblastoma
Neurons Mouse Fresh dissection
Nuclei Human Frozen lung tumor
Nuclei Human Frozen breast cancer
PBMCs Human Blood
Planaria SC Planarium Stem cells
Primary cells Human Fetal cortex
Primary cells Mouse Bone marrow
Primary cells Mouse Cardiomyocyte (adult)
Primary cells Mouse Embryos
Primary cells Mouse Retina
Primary FACS-sorted
cells
Mouse Lung epithelia
Scheider S2 Drosophila Embryo
SK-BR3 Human Breast
Skin Zebrafish Skin
Spheroids Human MCF10CA-derived
U-87-MG Human Glioblastoma; astrocytoma
UTHSC Human Bone marrow EW-8 Ewing Sarcoma
Z-138 Human B-cell lymphoma