ICELL8 cx system and software notices
At Takara Bio, we are continuously improving our automation offerings to help you make a difference using our best-in-class products. Therefore, it is important for us to share any known software bugs or new workflow practices as we learn of them. Our goal is to help you continue your valuable research while we implement new updates.
The issues below are known problems that might be experienced or seen with the ICELL8 cx Single-Cell System or in CELLSTUDIO or CellSelect v2.0 softwares. If you would like to receive email notifications in the future when this page is updated, please fill out the form on the left sidebar*.
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ICELL8 cx CELLSTUDIO v2.0 Software
Bug: loss of data if using 'All three' filter configuration for imaging
Version affected
CELLSTUDIO Software v2.0.9
Problem description
Some CELLSTUDIO Software installations include an invalid imaging filter configuration option, 'All three'. If your install is affected by this:
- The option will display under the Advanced tab > [Manage filter configurations...].
- While running a chip through an application workflow, ‘All three' may display in the "Optical configuration" drop-down menu in the Select dye configuration window.
Impact
If this option is selected during the imaging stage, the resulting image files become unreadable by CellSelect Software, causing a loss of data from the chip run.
Fix
This issue was fixed in CELLSTUDIO Software v2.0.19 (released: 06 March 2020).
Please contact Technical Support for the instructions to download and upgrade to the most recent version of CELLSTUDIO Software or to request an upgrade at your next scheduled yearly maintenance site visit.
Recommendation: avoid using green channel for imaging
Issue description
Although green (Ex: 474/50 nm, Em: 524/24 nm) is a color option listed in the Filter configuration editor window (Advanced > [Manage filter configurations...]), green channel imaging is not yet fully optimized and not recommended to use.
Impact
Using the green channel to image may result in issues distinguishing artifacts from dim cells.
Recommendation
When imaging with either blank or preprinted chips, use optical filters defined only with red, blue, or red/blue.
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