What's new
New to single-cell RNA-seq? These 5 tips will help you get started.
12/12/2018
2018 was a big year for science and for Takara Bio, with the launch of our new website and a full-fledged blog! From tips for setting up successful CRISPR experiments to RNA-seq kit comparisons for working with FFPE tissue, here are a few of the exciting Takara Bio developments that you may have missed.
12/11/2018
Differentiate yourself with a career at Takara Bio! In this career spotlight, meet field applications scientist Dr. Shuwen Chen, who talks about her career path and how she landed at Takara Bio.
12/03/2018
Read about how to find a CMO partner for your downstream clinical cell therapy applications.
11/09/2018
Featured information
In this webinar, Dr. Nathalie Bolduc (Takara Bio USA, Inc.) presents data from the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, which generates Illumina-ready sequencing libraries from 250 pg–10 ng mammalian total RNA inputs of any quality, including FFPE samples.
Our start-to-finish editing and single-cell cloning kits make human iPS cell editing routine instead of frustrating. In this video protocol, we provide step-by-step instructions for generating edited human iPS cell lines using our complete system for electroporation-based delivery of RNP complexes and single-cell cloning. This high-efficiency workflow greatly improves single-cell survival post-seeding, thanks to the Cellartis DEF-CS culture system. In addition to our electroporation-based kit, we also offer an alternate kit that uses gesicles to deliver RNP complexes.
See how Capturem Protein A high-capacity membranes streamline antibody screening workflows.
Learn about a novel solution for recombinant protein purification from mammalian and bacterial cell lysates.
Discover how Capturem Trypsin enables rapid on-column protein digestion into peptides suitable for proteomic analysis.
Workflow for generating edited, clonal hiPS cell lines using gesicle-based delivery of editing machinery.
Studying diabetes and metabolic disorders using hiPSC-derived beta cells instead of primary islets.
See data on how to easily and consistently create your own hepatocytes.
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Generate long ssDNA for CRISPR/Cas knockins with a simple in vitro protocol.
Rapidly detect single-nucleotide substitutions in edited cells before sequencing.
Perform pooled genome-wide CRISPR knockout screens in human cells.
Easily design primers for In-Fusion Cloning
Our NEW In-Fusion Cloning Primer Design Tool allows for single- or multiple-insert cloning, accommodates vector linearization by inverse PCR or restriction digest, and enables site-directed mutagenesis. Simply input the DNA sequences of your vector and insert(s), along with your linearization method to generate primers for your next cloning experiment. Easily switch to the mutagenesis option to generate primers for all of your insertion, replacement, and deletion projects.
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A new method for SMARTer library preparation from challenging RNA samples
In this webinar, Dr. Nathalie Bolduc (Takara Bio USA, Inc.) presents data from the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, which generates Illumina-ready sequencing libraries from 250 pg–10 ng mammalian total RNA inputs of any quality, including FFPE samples.
Video protocol: editing human iPSCs with CRISPR/Cas9 and single-cell cloning
Our start-to-finish editing and single-cell cloning kits make human iPS cell editing routine instead of frustrating. In this video protocol, we provide step-by-step instructions for generating edited human iPS cell lines using our complete system for electroporation-based delivery of RNP complexes and single-cell cloning. This high-efficiency workflow greatly improves single-cell survival post-seeding, thanks to the Cellartis DEF-CS culture system. In addition to our electroporation-based kit, we also offer an alternate kit that uses gesicles to deliver RNP complexes.
