Learning centers
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Automation systems
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SmartChip Real-Time PCR System introduction
- SmartChip Real-Time PCR System overview
- SmartChip Real-Time PCR System technical specifications
- SmartChip Real-Time PCR System applications
- SmartChip Real-Time PCR System video resources
- Request a SmartChip system demo
- Apollo library prep system introduction
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ICELL8 introduction
- ICELL8 technology overview
- ICELL8 cx technical specifications
- ICELL8 technical specifications (original system)
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Technical notes
- Basic cell preparation for the ICELL8 cx system
- ICELL8 cx system target enrichment for fusions
- ICELL8 cx system reagent formulation and dispense guidelines
- Protocol: High-throughput single cell DNA-seq
- Improved detection of gene fusions, SNPs, and alternative splicing
- Full-length transcriptome analysis
- Protocol: High-throughput single-cell ATAC-Seq
- High-throughput single-cell ATAC-seq
- Single-cell identification with CellSelect Software
- Single-cell analysis elucidates cardiomyocyte differentiation from iPSCs
- Combined TCR profiling and 5’ DE in single cells
- Automated, high-throughput TCR profiling
- Sample preparation protocols
- Video resources
- Citations
- Posters
- System & software notices
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SmartChip Real-Time PCR System introduction
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Next-generation sequencing
- Product line overview
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Technical notes
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Single-cell RNA- and DNA-seq
- Highest sensitivity for single-cell mRNA-seq
- Stranded libraries from single cells
- Streamlined single-cell mRNA-seq
- Full-length mRNA libraries from single cells (SMART-Seq v4)
- 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
- Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
- Full-length single-cell library method comparison
- High-resolution CNV detection using PicoPLEX Gold DNA-Seq
- Next-gen WGA method for CNV and SNV detection from single cells
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RNA-seq
- Stranded libraries from picogram-input total RNA (v3)
- Stranded libraries from picogram-input total RNA (v2)
- Stranded libraries from FFPE inputs (v2)
- Stranded libraries from 100 ng - 1 ug total RNA
- Stranded libraries from 100 pg-100 ng total RNA
- Stranded libraries from picogram-input total RNA (v1)
- Stranded RNA-seq competitor kit comparison
- Nonstranded libraries from FFPE inputs
- Sensitive capture of full-length transcript information with targeted RNA-seq
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DNA-seq
- ThruPLEX HV outperforms NEBNext Ultra II
- Accurate detection of low-frequency variants using molecular tags
- Streamlined DNA-seq from challenging samples
- Low cell number ChIP-seq using SMARTer ThruPLEX
- Cell-free DNA sequencing
- Sequencing analysis of low-frequency mutations in cfDNA
- DNA-seq from FFPE samples
- Low-input whole-exome sequencing
- Tag-seq variant detection
- Low-volume DNA shearing for SMARTer ThruPLEX library prep
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Immune profiling
- BCR repertoire profiling from human samples (bulk)
- Improved TCR repertoire profiling from human samples (bulk)
- TCR repertoire profiling from human samples (single cells)
- TCR repertoire profiling from human samples (bulk)
- TCR repertoire profiling from mouse samples (bulk)
- BCR repertoire profiling from mouse samples (bulk)
- Epigenetics and smRNA-seq
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Single-cell RNA- and DNA-seq
- Featured kits
- Technology and application overviews
- FAQs and tips
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DNA-seq protocols
- Using UMIs with ThruPLEX Tag-Seq HV
- Exome capture with Illumina Nextera Rapid Capture
- Targeted capture with Roche NimbleGen SeqCap EZ
- Targeted capture with IDT xGen panels
- Targeted capture with Agilent SureSelectXT
- Targeted capture with Agilent SureSelectXT2
- Targeted capture with Agilent SureSelectQXT
- Plasma preparation for ThruPLEX Plasma-Seq workflow
- Bioinformatics resources
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Webinars
- Investigating human health and disease at single-cell resolution
- TCR-seq methods: when to use which
- Taking single-cell RNA-seq by STORM
- Immunogenomics to accelerate immunotherapy
- Total RNA sequencing of liquid biopsies
- MeD-Seq, a novel method to detect DNA methylation
- Automating full-length single-cell RNA-seq libraries
- Single-cell DNA-seq
- Single-cell whole transcriptome analysis
- Citations
- Posters
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Gene function
- Gene editing
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Viral transduction
- Recombinant virus comparison
- Product finder
- Transduction posters
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Lentivirus
- Lenti-X FAQs
- Lentiviral workflow
- Lentiviral products guide
- Lentivirus biosafety
- Lentiviral tips
- Lentiviral vectors
- Lenti-X packaging
- High-throughput lentivirus production
- Lenti-X Concentrator
- Lentiviral titration
- Lenti-X GoStix Plus video protocols
- Lenti-X GoStix Plus FAQs
- Rapid lentivirus titration by p24 ELISA
- Lentiviral particles
- Lentiviral particles—fluorescent
- Lentiviral particles webinar
- Lentiviral particles FAQs
- Retrovirus
- Adeno-associated virus
- Adenovirus
- T-cell transduction and culture
- Inducible systems
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Transfection reagents
- Xfect Transfection Reagent
- Transfection tips
- Cell lines transfected with the Xfect reagent
- Protein transfection FAQs
- Xfect RNA Transfection Reagent
- Transfecting with single shots video
- Single shots picture protocol
- Transfection reagent products
- Protocol: transfection of cerebellar slice cultures with Xfect reagent
- Calcium phosphate transfection of neurons
- Fluorescent proteins
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Stem cell research
- Protocols
- Applications
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Technical notes
- Pluripotent stem cells
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Gene editing in hiPS cells
- Tagging an endogenous gene with AcGFP1 in hiPS cells
- Tagging an endogenous gene with a myc tag in hiPS cells
- Generating clonal hiPS cell lines deficient in CD81
- Introducing a tyrosinemia-related SNP in hiPS cells
- Inserting an expression cassette into the AAVS1 locus in hiPS cells
- Editing hiPS cells using electroporation
- Editing hiPS cells using gesicle technology
- Single-cell cloning of hiPS cells
- Organoids
- Beta cells
- Hepatocytes
- Cardiomyocytes
- Neural stem cells
- Posters
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Webinars
- Beta cells for disease modeling
- Efficient generation of hiPSC-derived disease model for primary hyperoxaluria type 1
- Edited hiPS cell lines for disease modeling
- Power medium for intrinsic clearance studies
- Exploring the potential of a hiPSC-derived hepatocyte disease model for NASH using bioinformatics
- Videos
- FAQs
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Citations
- Cellartis MSC Xeno-Free Culture Medium
- Cellartis Power Primary HEP Medium
- Cellartis DEF-CS 500 Culture System
- Cellartis Enhanced hiPS-HEP cells
- Cellartis hES-MP 002.5
- Cellartis hPS cell-derived cardiomyocytes
- Cellartis iPS Cell to Hepatocyte Differentiation System
- GS1-R
- 2i mES/iPSC medium
- iMatrix-511
- 3i mES/iPSC medium
- NDiff 227
- NDiff N2
- RHB-A
- STEM101
- STEM121
- STEM123
- Selection guides
- Overview
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Protein research
- Capturem technology
- Antibody purification
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His-tag purification
- Why tag a protein?
- Tech note: cobalt resin
- Purification methods overview
- Simplified purification of active, secreted his-tagged proteins
- Overview: His60
- Tech note: Capturem technology
- Tech note: Capturem large volume
- Magnetic beads
- TALON resin selection guide
- Selection guide: His60 resin
- FAQs: TALON
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Protocols
- Video: Capturem his maxiprep
- Video: Capturem his miniprep
- Visual protocol: Capturem his maxiprep
- Visual protocol: Capturem his miniprep
- Capturem nickel column reagent compatibility
- TALON reagent compatibility
- His60 reagent compatibility
- TALON: Native vs denaturing purification
- Protocol: denaturing purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, pH elution
- Other tag purification
- Phosphoprotein and glycoprotein purification
- Mass spectrometry digestion reagents
- Matchmaker Gold yeast two-hybrid systems
- Expression systems
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PCR
- Citations
- Selection guides
- PCR enzyme brochure
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Technical notes
- Rapid, high-performance multiplex PCR
- EmeraldAmp outperforms MyTaq Red mix
- Fast and accurate PCR
- Methylation studies
- Hot-start PCR
- Long-range PCR with LA Taq
- Direct PCR from human nail
- Direct PCR from meat samples
- Megaprimer PCR with PrimeSTAR GXL
- Amplifying GC-rich templates
- Titanium Taq for high-throughput genotyping
- Colony PCR in under an hour
- High-throughput endpoint PCR
- Direct PCR from blood
- PrimeSTAR GXL for targeted sequencing
- Detecting somatic mosaicism using massively parallel sequencing
- PCR FAQs
- Go green with lyophilized enzymes
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Cloning
- In-Fusion Cloning: general information
- Primer design and other tools
- In‑Fusion Cloning tips and FAQs
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Applications and tech notes
- In-Fusion Cloning applications collection
- Efficient multiple-fragment cloning
- Mutagenesis with In-Fusion Cloning
- Rapid, high-throughput cloning for antibody development
- Solve a synthesis challenge with easy multiple-insert cloning
- Direct cloning into large vectors
- Simplified insertion of a GFP-encoding cassette into a 100-kb plasmid
- Efficient cloning for sgRNA/Cas9 plasmids
- In-Fusion Cloning of sgRNAs
- De novo insertion of small fusion protein tags
- Sign up to stay updated
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Traditional molecular cloning
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Restriction enzyme overview
- General information about restriction enzymes
- Star activity of restriction enzymes
- Inactivation of restriction enzymes
- Buffer activity with restriction enzymes
- Universal buffers for double digestion with restriction enzymes
- Restriction enzymes affected by methylation
- Methylation-sensitive restriction enzymes
- QC of restriction enzymes
- Ligation cloning overview
- Ligation product guide
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Restriction enzyme overview
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Nucleic acid purification
- Product finder
- Plasmid purification
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Genomic DNA purification
- NucleoSpin Tissue
- NucleoSpin DNA RapidLyse
- NucleoSpin Tissue XS
- NucleoSnap cfDNA
- NucleoSpin cfDNA XS
- NucleoMag cfDNA
- NucleoMag Pathogen
- NucleoMag Virus
- NucleoMag Blood
- NucleoBond HMW DNA
- NucleoMag DNA FFPE
- NucleoSpin 96 DNA FFPE
- NucleoMag DNA Swab
- NucleoMag VET
- NucleoMag 384 Plant
- NucleoSpin cfDNA Midi
- DNA/RNA cleanup and extraction
- Parallel DNA, RNA & protein
- Automated DNA and RNA purification
- RNA purification
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Hard-to-lyse samples
- NucleoMag DNA Bacteria
- NucleoSpin Plant II
- NucleoSpin RNA Plant and Fungi
- NucleoMag DNA Food
- NucleoSpin DNA Insect
- NucleoSpin Soil
- NucleoSpin 8/96 Soil
- NucleoBond RNA Soil
- NucleoSpin DNA Lipid Tissue
- NucleoSpin DNA Stool
- NucleoSpin RNA Stool
- NucleoSpin Microbial DNA
- NucleoMag DNA/RNA Water
- Sample homogenization beads
- Antibodies and ELISA
- Cell biology assays
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Real-time PCR
- Product finder
- Reaction size guidelines for qPCR
- Real-time PCR products brochure
- Real-time PCR tutorial videos
- Guest webinar: extraction-free SARS-CoV-2 detection
- Overview
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Technical notes
- Unbiased preamplification of limited samples
- Accurate gene expression analysis with TB Green Premix Ex Taq II
- Efficient quantification of human gene expression with PrimeScript Reverse Transcriptase
- Rapid qPCR analysis from blood samples using PrimeScript Reverse Transcriptase
- Monitoring siRNA knockdown
- qPCR without optimization using TB Green Premix Ex Taq
- Fast synthesis of cDNA templates for real-time RT-PCR
- Specific, consistent real-time PCR with TB Green Premix Ex Taq II
- FAQs
- cDNA synthesis
Next-generation sequencing learning center
Next-generation sequencing (NGS) has revolutionized our understanding of biological phenomena and human disease by enabling analyses of gene expression, gene regulation, and genetic variation at unprecedented scales. As NGS technology continues to evolve and new applications are developed, Takara Bio produces an array of informational resources, including technical notes, webinars, scientific posters (and more!), intended to promote awareness about the latest NGS innovations and capabilities.
Technical notes
Technical notes for a wide variety of NGS applications.
Featured kits
An overview of highlighted SMARTer NGS products.
FAQs and tips
Helpful information for successful NGS experiments.
Protocols
Currently featuring protocols for integrating SMARTer ThruPLEX kits with leading target enrichment systems.
Webinars
Prerecorded webinars covering a variety of NGS applications.
Citations
NGS citation lists feature good science done using Takara Bio SMARTer NGS tools.
Technology overviews
Overviews of SMARTer NGS technologies and applications.
NGS posters
Posters featuring SMARTer NGS methods and data.
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