We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About

Close

  • ‹ Back to Learning centers
  • Automation systems
  • Next-generation sequencing
    • Product line overview
    • Technical notes
    • Featured kits
    • Technology and application overviews
    • FAQs and tips
    • DNA-seq protocols
    • Bioinformatics resources
    • Newsletters
    • Webinars
    • Citations
    • Posters
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
Home › Learning centers › Next-generation sequencing

Learning centers

  • Automation systems
    • SmartChip Real-Time PCR System introduction
      • SmartChip Real-Time PCR System overview
      • SmartChip Real-Time PCR System technical specifications
      • SmartChip Real-Time PCR System applications
        • Antibiotic resistance genes
          • Screening for antibiotic resistance genes in manure and sewage
          • Uncovering antibiotic resistance genes in soil, sediment, and sludge
          • Tracking down antibiotic resistance genes in hospitals
          • Identifying antibiotic resistance genes in water
        • mRNA, miRNA, and lncRNA as disease biomarkers
        • Pathogen detection in human samples and food
        • Genotyping using animal and blood samples
      • SmartChip Real-Time PCR System video resources
        • Detection of ARGs and SARS-CoV-2 in wastewater
        • Antibiotic resistance screening webinar
      • Request a SmartChip system demo
    • Apollo library prep system introduction
      • Automated VeriSeq library preparation for PGS
      • Apollo library prep system citations
      • SMART-Seq v4 chemistry for the SMARTer Apollo system
      • Apollo library prep system overview
      • Apollo system technical specifications
      • In-tip bead separation on the Apollo system
    • ICELL8 introduction
      • ICELL8 technology overview
      • ICELL8 cx technical specifications
      • ICELL8 technical specifications (original system)
      • ICELL8 vs plate-seq
      • Webinars
      • Technical notes
        • Basic cell preparation for the ICELL8 cx system
        • ICELL8 cx system target enrichment for fusions
        • ICELL8 cx system reagent formulation and dispense guidelines
        • Protocol: High-throughput single cell DNA-seq
        • Improved detection of gene fusions, SNPs, and alternative splicing
        • Full-length transcriptome analysis
        • Protocol: High-throughput single-cell ATAC-Seq
        • High-throughput single-cell ATAC-seq
        • Single-cell identification with CellSelect Software
        • Single-cell analysis elucidates cardiomyocyte differentiation from iPSCs
        • Combined TCR profiling and 5’ DE in single cells
        • Automated, high-throughput TCR profiling
      • Sample preparation protocols
        • Protocol: Oxford Nanopore library prep and quantification
        • Protocol: Nuclei isolation from mammalian cells
        • Protocol: Mouse cardiomyocyte preparation
        • Isolate cells of any size
      • Video resources
      • Citations
      • Posters
      • System & software notices
  • Next-generation sequencing
    • Product line overview
    • Technical notes
      • Single-cell RNA- and DNA-seq
        • All-in-one cDNA synthesis and library prep from single cells
        • Highest sensitivity for single-cell mRNA-seq
        • Stranded libraries from single cells
        • Streamlined single-cell mRNA-seq
        • Full-length mRNA libraries from single cells (SMART-Seq v4)
        • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
        • Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
        • Full-length single-cell library method comparison
        • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
        • Next-gen WGA method for CNV and SNV detection from single cells
      • RNA-seq
        • Stranded libraries from picogram-input total RNA (v3)
        • Automation-friendly, all-in-one cDNA synthesis and library prep
        • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
        • Stranded libraries from picogram-input total RNA (v2)
        • Stranded libraries from FFPE inputs (v2)
        • Stranded libraries from 100 ng - 1 ug total RNA
        • Stranded libraries from 100 pg-100 ng total RNA
        • Stranded libraries from picogram-input total RNA (v1)
        • Stranded RNA-seq competitor kit comparison
        • Nonstranded libraries from FFPE inputs
        • Sensitive capture of full-length transcript information with targeted RNA-seq
      • DNA-seq
        • Comparing ThruPLEX HV PLUS to Kapa and NEBNext
        • Improvements to ThruPLEX HV
        • ThruPLEX HV outperforms NEBNext Ultra II
        • Accurate detection of low-frequency variants using molecular tags
        • Streamlined DNA-seq from challenging samples
        • Low cell number ChIP-seq using SMARTer ThruPLEX
        • Cell-free DNA sequencing
        • Sequencing analysis of low-frequency mutations in cfDNA
        • DNA-seq from FFPE samples
        • Low-input whole-exome sequencing
        • Tag-seq variant detection
        • Low-volume DNA shearing for SMARTer ThruPLEX library prep
      • Immune profiling
        • BCR repertoire profiling from human samples (bulk)
        • Improved TCR repertoire profiling from human samples (bulk)
        • TCR repertoire profiling from human samples (single cells)
        • TCR repertoire profiling from human samples (bulk)
        • TCR repertoire profiling from mouse samples (bulk)
        • BCR repertoire profiling from mouse samples (bulk)
      • Epigenetics and smRNA-seq
        • Full-length small RNA libraries
        • ChIP-seq libraries for transcription factor analysis
        • ChIP-seq libraries from ssDNA
        • Methylated DNA-seq
    • Featured kits
    • Technology and application overviews
      • SMART technology
      • Single-cell mRNA-seq
      • Total RNA-seq
      • Biomarker discovery with RNA-seq
      • SMART-Seq PLUS solutions
      • Whole genome amplification from single cells
      • Cell-free nucleic acid sequencing
      • ThruPLEX HV data sheet
      • Sequencing depth for ThruPLEX Tag-seq
    • FAQs and tips
      • RNA-seq FAQs
      • RNA-seq tips
      • Positive and negative controls in scRNA-seq
      • DNA-seq FAQs
      • ChIP-seq FAQs
      • Indexing FAQs
    • DNA-seq protocols
      • Using UMIs with ThruPLEX Tag-Seq HV
      • Exome capture with Illumina Nextera Rapid Capture
      • Targeted capture with Roche NimbleGen SeqCap EZ
      • Targeted capture with IDT xGen panels
      • Targeted capture with Agilent SureSelectXT
      • Targeted capture with Agilent SureSelectXT2
      • Targeted capture with Agilent SureSelectQXT
      • Plasma preparation for ThruPLEX Plasma-Seq workflow
    • Bioinformatics resources
      • Cogent NGS Immune Profiler Software
        • Cogent NGS Immune Profiler Software notices
        • Cogent NGS Immune Profiler Software videos
      • ICELL8 scTCR Analyzer
      • SMARTer Human scTCR Demultiplexer
      • Cogent NGS Analysis Pipeline
        • Cogent NGS Analysis Pipeline v1.0 User Manual
        • Cogent NGS Analysis Pipeline notices
      • Cogent NGS Discovery Software
        • Cogent NGS Discovery Software v1.0 User Manual
        • Cogent NGS Discovery Software notices
      • SMART-Seq DE3 Demultiplexer
    • Newsletters
      • 2020 NGS newsletter
    • Webinars
      • Single-Cell Workshop
      • TCR-seq methods: when to use which
      • Taking single-cell RNA-seq by STORM
      • Immunogenomics to accelerate immunotherapy
      • Total RNA sequencing of liquid biopsies
      • MeD-Seq, a novel method to detect DNA methylation
      • Automating full-length single-cell RNA-seq libraries
      • Single-cell DNA-seq
      • Single-cell whole transcriptome analysis
    • Citations
      • Epigenetics citations
      • Microbiome citations
      • PicoPLEX citations
      • SMARTer RNA-seq citations
      • ThruPLEX DNA-seq citations
      • ThruPLEX Plasma-seq citations
    • Posters
  • Gene function
    • Gene editing
      • Gene editing product finder
      • Gene editing tools and information
        • sgRNA design tools
        • Tools for successful CRISPR/Cas9 genome editing
        • Gene editing posters
        • Customer data for Guide-it products
        • How to design sgRNA sequences
        • Introduction to the CRISPR/Cas9 system
        • Gene editing of CD3+ T cells and CD34+ HSCs
      • CRISPR/Cas9 knockouts
        • Mutation detection kit comparison
        • Screening for effective guide RNAs
        • Monoallelic versus biallelic mutants
        • Indel identification kit for mutation characterization
      • CRISPR/Cas9 knockins
        • Choosing an HDR template format
        • Homology-directed repair FAQs
        • Mouse CRISPR knockin protocol
        • Site-specific gene knockins using long ssDNA
        • Efficient CRISPR/Cas9-mediated knockins in iPS cells
        • Oligo design tool for detecting precise insertions
          • Oligo design tool user guide (insertions)
      • Genome-wide screening
        • CRISPR library screening
        • CRISPR library screening webinar
        • Phenotypic screen using sgRNA library system
      • Creating and screening for SNPs
        • SNP detection with knockin screening kit
        • Oligo design tool for SNP screening
          • Oligo design tool user guide (SNPs)
        • Sign up: SNP engineering webinar
        • Guide-it SNP Screening Kit FAQs
      • CRISPR/Cas9 delivery methods
        • Electroporation-grade Cas9 for editing in diverse cell types
        • CRISPR/Cas9 gene editing with AAV
        • CRISPR/Cas9 gesicles overview
        • Cas9 Gesicles—reduced off-target effects
        • sgRNA-Cas9 delivery to many cell types
        • Tet-inducible Cas9 for gene editing
      • Cre recombinase
        • Control your Cre recombinase experiments
        • Fast Cre delivery with gesicle technology
    • Viral transduction
      • Recombinant virus comparison
      • Product finder
      • Transduction posters
      • Lentivirus
        • Lenti-X FAQs
        • Lentiviral workflow
        • Lentiviral products guide
        • Lentivirus biosafety
        • Lentiviral tips
        • Lentiviral vectors
        • Lenti-X packaging
        • High-throughput lentivirus production
        • Lenti-X Concentrator
        • Lentiviral titration
        • Lenti-X GoStix Plus video protocols
        • Lenti-X GoStix Plus FAQs
        • Rapid lentivirus titration by p24 ELISA
        • Lentiviral particles
        • Lentiviral particles—fluorescent
        • Lentiviral particles webinar
        • Lentiviral particles FAQs
      • Retrovirus
        • Retroviral products
        • Retro-X FAQs
        • Retro-X packaging
        • Retro-X Concentrator
      • Adeno-associated virus
        • AAV workflow
        • AAV products
        • AAV FAQs
        • AAV tech notes
          • Customer data: Purified AAV9 delivery (mouse brain)
          • Customer data: Purified AAV9 delivery (songbird brain)
          • Serotype-independent AAV vector purification
          • Customer data: Purified AAV2 delivery (mouse brain)
        • AAV videos
          • AAV2 purification video
          • AAVpro "All Serotypes" protocols
        • AAVpro Concentrator overview
      • Adenovirus
        • Adenoviral FAQs
        • Adenoviral products
        • Fastest, easiest adenoviral system ever
        • Tet-inducible adenovirus
        • Adenovirus purification kits
        • Adenovirus purification mega-scale
        • Adenovirus rapid titer
        • Adenoviral titration
    • T-cell transduction and culture
      • Technology overview
      • Adoptive T-cell therapy (ACT)
      • RetroNectin FAQs
      • Hematopoietic cell transduction
      • T-cell expansion
      • Serum-free T-cell culture
      • CultiLife culture bags protocol
    • Inducible systems
      • iDimerize systems
        • Inducible protein-protein interactions—iDimerize systems
        • iDimerize systems journal club
        • iDimerize in vivo protocol
        • iDimerize systems citations
        • ARGENT cell signaling regulation kits from ARIAD
      • ProteoTuner systems
        • ProteoTuner technology overview
        • ProteoTuner citations
        • Inducible protein stabilization systems product selection guide
      • Tet-inducible systems
        • Tet systems product selection guide
        • Tet systems overview
        • Tet-One technology overview
        • Tet-On 3G plasmid system kit components
        • Tet-On 3G lentiviral system kit components
        • Tet system webinars
    • Transfection reagents
      • Xfect Transfection Reagent
      • Transfection tips
      • Cell lines transfected with the Xfect reagent
      • Protein transfection FAQs
      • Xfect RNA Transfection Reagent
      • Transfecting with single shots video
      • Single shots picture protocol
      • Transfection reagent products
      • Protocol: transfection of cerebellar slice cultures with Xfect reagent
      • Calcium phosphate transfection of neurons
    • Fluorescent proteins
      • Fluorescent protein vector finder
      • Fluorescent protein excitation and emission maxima
      • Fluorescent protein antibodies selection guide
      • Fluorescent protein antibody citations
        • GFP antibody citations
        • RFP antibody citations
      • Fluorescent protein quick guide
      • Fluorescent retroviral expression vectors
      • Verify miRNA expression
      • Find EGFP vector alternatives
  • Stem cell research
    • Protocols
      • Hepatocytes
        • Video protocols for hiPS-HEP v2 cells
        • Getting started with hepatocyte differentiation
      • Pluripotent stem cells
        • Transferring iPSCs on MEFs to DEF-CS
        • Transferring iPSCs from other media to DEF-CS
        • Spin embryoid body formation
        • Reprogramming PBMCs
        • Reprogramming fibroblasts
      • Cardiomyocytes
        • Cardiomyocytes in FLIPR 384-well plate format
        • Cardiomyocytes on the Patchliner system
        • Cardiomyocytes on the Maestro MEA system
        • Cardiomyocytes on the MED64 MEA system
        • Cardiomyocytes on the CardioExcyte 96 system
        • Cardiomyocytes on the xCELLigence RTCA CardioECR system
    • Applications
    • Technical notes
      • Pluripotent stem cells
        • Using the DEF-CS system to culture human iPS cells
        • Comparison of the Cellartis DEF-CS system with other vendors' human iPS cell culture systems
        • Reprogramming PBMCs
        • Reprogramming fibroblasts
      • Gene editing in hiPS cells
        • Tagging an endogenous gene with AcGFP1 in hiPS cells
        • Tagging an endogenous gene with a myc tag in hiPS cells
        • Generating clonal hiPS cell lines deficient in CD81
        • Introducing a tyrosinemia-related SNP in hiPS cells
        • Inserting an expression cassette into the AAVS1 locus in hiPS cells
        • Editing hiPS cells using electroporation
        • Editing hiPS cells using gesicle technology
        • Single-cell cloning of hiPS cells
      • Organoids
        • Generation of embryonic organoids using NDiff 227 neural differentiation medium
      • Beta cells
        • Beta cells for disease modeling
      • Hepatocytes
        • hiPS-HEP cells for disease modeling
        • hiPS-HEP cells for drug metabolism studies
        • Power medium for long-term human primary hepatocyte culture
        • iPS cell to hepatocyte differentiation system
      • Cardiomyocytes
        • Making engineered heart tissue with cardiomyocytes
      • Neural stem cells
        • RHB-A neural stem cell medium
    • Posters
    • Webinars
      • Beta cells for disease modeling
      • Efficient generation of hiPSC-derived disease model for primary hyperoxaluria type 1
      • Edited hiPS cell lines for disease modeling
      • Power medium for intrinsic clearance studies
      • Exploring the potential of a hiPSC-derived hepatocyte disease model for NASH using bioinformatics
    • Videos
      • Hepatocyte offerings
    • FAQs
      • Cellartis DEF-CS 500 Culture System FAQs
      • Cellartis enhanced hiPS-HEP FAQs
      • Cellartis iPS Cell to Hepatocyte Differentiation System FAQs
    • Citations
      • Cellartis MSC Xeno-Free Culture Medium
      • Cellartis Power Primary HEP Medium
      • Cellartis DEF-CS 500 Culture System
      • Cellartis Enhanced hiPS-HEP cells
      • Cellartis hES-MP 002.5
      • Cellartis hPS cell-derived cardiomyocytes
      • Cellartis iPS Cell to Hepatocyte Differentiation System
      • GS1-R
      • 2i mES/iPSC medium
      • iMatrix-511
      • 3i mES/iPSC medium
      • NDiff 227
      • NDiff N2
      • RHB-A
      • STEM101
      • STEM121
      • STEM123
    • Selection guides
      • Stem cell antibody selection guide
      • Stem cell media product finder
      • Stem cell tools product finder
      • Hepatocyte product finder
    • Overview
      • Stem cell research products and services
      • Stem cell media products
      • Cardiomyocyte products
      • Hepatocyte products and services
      • iPS cell to hepatocyte differentiation overview
      • Cellartis human pluripotent stem cell services
  • Protein research
    • Capturem technology
      • Capturem protocols
      • Capturem tech notes and applications
      • FAQs about Capturem technology
      • Capturem technology citations
      • Capturem posters
    • Antibody purification
      • Tech note: Rapid Protein G-based antibody purification
      • Tech note: thiophilic antibody purification resins
      • Antibody purification and screening with Capturem Protein A
      • Tech note: IP and Co-IP
      • Rapid antibody labeling with Capturem technology
    • His-tag purification
      • Why tag a protein?
      • Tech note: cobalt resin
      • Purification methods overview
      • Simplified purification of active, secreted his-tagged proteins
      • Overview: His60
      • Tech note: Capturem technology
      • Tech note: Capturem large volume
      • Magnetic beads
      • TALON resin selection guide
      • Selection guide: His60 resin
      • FAQs: TALON
      • Protocols
        • Video: Capturem his maxiprep
        • Video: Capturem his miniprep
        • Visual protocol: Capturem his maxiprep
        • Visual protocol: Capturem his miniprep
        • Capturem nickel column reagent compatibility
        • TALON reagent compatibility
        • His60 reagent compatibility
        • TALON: Native vs denaturing purification
        • Protocol: denaturing purification with TALON resin, imidazole elution
        • Protocol: native purification with TALON resin, imidazole elution
        • Protocol: native purification with TALON resin, pH elution
    • Other tag purification
      • Streptavidin-based enrichment using Capturem technology
      • Selection guide: peptide tags
      • Myc-tagged protein purification overview
      • GST-tagged protein purification overview
    • Phosphoprotein and glycoprotein purification
      • Non-tagged protein purification overview
      • Phosphoprotein purification overview
    • Mass spectrometry digestion reagents
      • Capturem technology for trypsin protein digestion
      • Protein and whole-cell lysate digestion with Capturem Trypsin technology
      • Protein digestion with Capturem Pepsin
    • Matchmaker Gold yeast two-hybrid systems
      • Matchmaker Gold Yeast Two-Hybrid System
      • Make your own library for yeast two-hybrid screening
      • Mate and Plate yeast two-hybrid cDNA libraries
      • Aureobasidin A for improved selectable drug resistance in yeast
    • Expression systems
      • Protein expression overview
      • Insect expression overview
      • Mammalian expression overview
      • pHEK293 Ultra expression overview
      • OKT3 expression in mammalian cells
      • Bacterial expression overview
  • PCR
    • Citations
      • PrimeSTAR HS
      • EmeraldAmp MAX
      • Terra PCR Direct
      • EmeraldAmp GT
      • Takara Ex Taq
      • PrimeSTAR Max
      • PrimeSTAR GXL
      • Takara LA Taq
      • SpeedSTAR HS
      • Takara Taq and Taq HS
      • Titanium Taq
    • Selection guides
      • PCR selection guide
      • Direct PCR selection guide
      • GPR-registered PCR products selection guide
      • Commercial-use PCR selection guide
      • Fast PCR selection guide
    • PCR enzyme brochure
    • Technical notes
      • Rapid, high-performance multiplex PCR
      • EmeraldAmp outperforms MyTaq Red mix
      • Fast and accurate PCR
      • Methylation studies
      • Hot-start PCR
      • Long-range PCR with LA Taq
      • Direct PCR from human nail
      • Direct PCR from meat samples
      • Megaprimer PCR with PrimeSTAR GXL
      • Amplifying GC-rich templates
      • Titanium Taq for high-throughput genotyping
      • Colony PCR in under an hour
      • High-throughput endpoint PCR
      • Direct PCR from blood
      • PrimeSTAR GXL for targeted sequencing
      • Detecting somatic mosaicism using massively parallel sequencing
    • PCR FAQs
      • Primer design
      • Optimization
      • Troubleshooting
      • Applications and conditions
      • Shipping, storage, and handling
    • Go green with lyophilized enzymes
  • Cloning
    • In-Fusion Cloning: general information
      • In-Fusion Cloning overview
      • In-Fusion Cloning guide
      • Choosing a seamless cloning method
      • In-Fusion Cloning and competition
        • Higher cloning efficiency using In-Fusion Snap Assembly
        • Sequence accuracy in seamless cloning
        • Improving background over Gibson Assembly
        • A successful alternative to ligation cloning
        • Single- and multiple-insert cloning
        • Easy cloning into lentiviral vectors
        • Outperforming TOPO cloning
      • In-Fusion Cloning citations
      • EcoDry reagents and sustainability
    • Primer design and other tools
      • Seamless cloning primer design
      • In-Fusion Cloning tutorials
        • Cloning one or more fragments
        • Deleting a sequence
        • Inserting a sequence
        • Deleting and replacing a sequence
      • In-Fusion molar ratio calculator
      • Simulate your construct
    • In‑Fusion Cloning tips and FAQs
    • Applications and tech notes
      • In-Fusion Cloning applications collection
      • Efficient multiple-fragment cloning
      • Mutagenesis with In-Fusion Cloning
      • Rapid, high-throughput cloning for antibody development
      • Solve a synthesis challenge with easy multiple-insert cloning
      • Direct cloning into large vectors
      • Simplified insertion of a GFP-encoding cassette into a 100-kb plasmid
      • Efficient cloning for sgRNA/Cas9 plasmids
      • In-Fusion Cloning of sgRNAs
      • De novo insertion of small fusion protein tags
    • Sign up to stay updated
    • Traditional molecular cloning
      • Restriction enzyme overview
        • General information about restriction enzymes
        • Star activity of restriction enzymes
        • Inactivation of restriction enzymes
        • Buffer activity with restriction enzymes
        • Universal buffers for double digestion with restriction enzymes
        • Restriction enzymes affected by methylation
        • Methylation-sensitive restriction enzymes
        • QC of restriction enzymes
      • Ligation cloning overview
      • Ligation product guide
  • Nucleic acid purification
    • Product finder
    • Plasmid purification
      • Plasmid kit selection guide
      • NucleoSpin Plasmid Transfection-grade
      • NucleoSnap Plasmid Midi
      • NucleoBond Xtra Midi/Maxi
    • Genomic DNA purification
      • NucleoSpin Tissue
      • NucleoSpin DNA RapidLyse
      • NucleoSpin Tissue XS
      • NucleoSnap cfDNA
      • NucleoSpin cfDNA XS
      • NucleoMag cfDNA
      • NucleoMag Pathogen
      • NucleoMag Virus
      • NucleoMag Blood
      • NucleoBond HMW DNA
      • NucleoMag DNA FFPE
      • NucleoSpin 96 DNA FFPE
      • NucleoMag DNA Swab
      • NucleoMag VET
      • NucleoMag 384 Plant
      • NucleoSpin cfDNA Midi
    • DNA/RNA cleanup and extraction
      • NucleoSpin Gel and PCR Clean-up
      • NucleoMag NGS Clean-up and Size Select
      • NucleoSpin RNA Clean-up
    • Parallel DNA, RNA & protein
      • NucleoSpin RNA/Protein
      • NucleoSpin TriPrep
    • Automated DNA and RNA purification
      • Application notes, by sample type
        • Plasmid purification
        • DNA cleanup
        • DNA from cells and tissues
        • RNA from cells and tissues
        • DNA from blood and bodily fluids
        • DNA and RNA from plants, fungi, and food
        • Viral RNA and DNA purification
      • Application notes, by platform
        • Eppendorf
        • Tecan
        • Hamilton
        • Thermo Fisher Scientific
        • MASMEC Biomed
    • RNA purification
      • RNA purification overview
      • RNA purification kit finder
      • NucleoSpin RNA Plus
      • NucleoSpin RNA Plus XS
      • NucleoSpin RNA
      • NucleoProtect RNA
      • NucleoSpin miRNA
      • NucleoSpin RNA Blood
      • NucleoSpin miRNA Plasma
      • NucleoZOL for RNA isolation
      • High-throughput RNA isolation from FFPE samples
    • Hard-to-lyse samples
      • NucleoMag DNA Bacteria
      • NucleoSpin Plant II
      • NucleoSpin RNA Plant and Fungi
      • NucleoMag DNA Food
      • NucleoSpin DNA Insect
      • NucleoSpin Soil
      • NucleoSpin 8/96 Soil
      • NucleoBond RNA Soil
      • NucleoSpin DNA Lipid Tissue
      • NucleoSpin DNA Stool
      • NucleoSpin RNA Stool
      • NucleoSpin Microbial DNA
      • NucleoMag DNA/RNA Water
      • NucleoSpin eDNA Water
      • Sample homogenization beads
  • Antibodies and ELISA
    • Osteocalcin focus
  • Cell biology assays
    • Extracellular vesicle isolation
    • Technical notes
      • Extracellular vesicle isolation from biofluids
      • Cell viability and proliferation measurement
      • Exosome isolation from cell culture
      • Mir-X microRNA quantification
    • FAQs
    • Citations
  • Real-time PCR
    • Product finder
    • Reaction size guidelines for qPCR
    • Real-time PCR products brochure
    • Real-time PCR tutorial videos
    • Guest webinar: extraction-free SARS-CoV-2 detection
    • Overview
      • One-step RT-qPCR kits
      • Two-step RT-qPCR kits
      • Probe-based qPCR kits
      • TB Green-based qPCR kits
      • Videos: qPCR analysis for challenging inputs
      • qRT-PCR to detect RNA present at low levels
    • Technical notes
      • Unbiased preamplification of limited samples
      • Accurate gene expression analysis with TB Green Premix Ex Taq II
      • Efficient quantification of human gene expression with PrimeScript Reverse Transcriptase
      • Rapid qPCR analysis from blood samples using PrimeScript Reverse Transcriptase
      • Monitoring siRNA knockdown
      • qPCR without optimization using TB Green Premix Ex Taq
      • Fast synthesis of cDNA templates for real-time RT-PCR
      • Specific, consistent real-time PCR with TB Green Premix Ex Taq II
    • FAQs
  • cDNA synthesis
    • Premium total and poly A+ RNA
    • SMARTer RACE 5'/3' Kit—advances in SMARTer PCR cDNA synthesis
    • Cloning antibody variable regions
New products
Need help?
Contact Sales

Next-generation sequencing learning center

Next-Gen Sequencing

Next-generation sequencing (NGS) has revolutionized our understanding of biological phenomena and human disease by enabling analyses of gene expression, gene regulation, and genetic variation at unprecedented scales. As NGS technology continues to evolve and new applications are developed, Takara Bio produces an array of informational resources, including technical notes, webinars, scientific posters (and more!), intended to promote awareness about the latest NGS innovations and capabilities.


Technical notes

Technical notes for a wide variety of NGS applications.

Featured kits

An overview of highlighted SMARTer NGS products.

FAQs and tips

Helpful information for successful NGS experiments.

Protocols

Currently featuring protocols for integrating SMARTer ThruPLEX kits with leading target enrichment systems.

Webinars

Prerecorded webinars covering a variety of NGS applications.

Citations

NGS citation lists feature good science done using Takara Bio SMARTer NGS tools.

Technology overviews

Overviews of SMARTer NGS technologies and applications.

NGS posters

Posters featuring SMARTer NGS methods and data.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2020 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
  • Corporate development
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2021 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Drug discovery
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Immune profiling
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Publications
  • Automation systems
  • SmartChip Real-Time PCR System, chips, and reagents
  • Apollo system
  • ICELL8 system and software
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Whole genome amplification
  • Immune profiling
  • Bioinformatics tools
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Human iPS cell gene editing systems
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • cDNA synthesis
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion Cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Cell biology assays
  • Extracellular vesicle isolation
  • Exosome isolation (cell culture)
  • Reporter systems
  • Apoptosis detection kits
  • Epigenetics
  • Signal transduction
  • Gene function
  • Gene editing
  • Fluorescent proteins
  • Viral transduction
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • GoStix Plus special offers
  • PCR samples
Vaccine development

Vaccine development

The rapid spread of severe infections by viruses such as SARS-CoV-2, HIV, H1N1, Ebola, and Zika has highlighted the critical need for the rapid development of vaccines against previously unknown pathogens to deal with pandemics such as COVID-19 effectively.

Takara Bio is proud to be on the front line in the fight to defeat the novel coronavirus by enabling innovative vaccine development. This section discusses tools and techniques to overcome the challenges faced during the vaccine development process.

Learn how our products help speed up vaccine development

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • Apollo library prep system introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Product line overview
  • Technical notes
  • FAQs and tips
  • Bioinformatics resources
  • Newsletters
  • Webinars
  • Citations
  • Posters
  • Real-time PCR
  • Product finder
  • Reaction size guidelines for qPCR
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Overview
  • Technical notes
  • FAQs
  • Stem cell research
  • Protocols
  • Applications
  • Technical notes
  • Webinars
  • Videos
  • Citations
  • Nucleic acid purification
  • Product finder
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • Automated DNA and RNA purification
  • RNA purification
  • Hard-to-lyse samples
  • cDNA synthesis
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • PCR FAQs
  • Cloning
  • In-Fusion Cloning: general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and tech notes
  • Cell biology assays
  • Extracellular vesicle isolation
  • Technical notes
  • FAQs
  • Citations
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Protein research
  • Capturem technology
  • Antibody purification
  • His-tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Antibodies and ELISA
Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Corporate development
  • Partnering & OEM solutions
  • In licensing
  • Out licensing
  • Submit a licensing request
  • Webinars
  • NGS: biomarkers and oncology
  • NGS: immunology
  • Stem cells
  • Real-time PCR
  • Gene function
  • Protein science
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

Learn more

  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Website FAQs
Best-in-class products, expert support, superior value

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
  • COVID-19 research
  • Drug discovery
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Immune profiling
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Publications
  • Automation systems
  • SmartChip Real-Time PCR System, chips, and reagents
  • Apollo system
  • ICELL8 system and software
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Whole genome amplification
  • Immune profiling
  • Epigenetics and small RNA sequencing
  • NGS accessories
  • Bioinformatics tools
  • Gene function
  • Gene editing
  • Fluorescent proteins
  • Viral transduction
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • ProteoTuner protein control systems
  • iDimerize inducible protein interaction systems
  • Transfection reagents
  • Mammalian expression plasmids
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Human iPS cell gene editing systems
  • Accessories
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Expression vectors & systems
  • Glycobiology
  • Antibodies and immunoprecipitation
  • SDS-PAGE & western blotting
  • Protein sequencing
  • Accessory enzymes
  • PCR
  • Most popular polymerases
  • Standard PCR
  • High-yield PCR
  • High-fidelity PCR
  • Fast PCR
  • Long-range PCR
  • GC rich PCR
  • Direct PCR
  • PCR master mixes
  • Custom business friendly and automation-ready solutions
  • Molecular diagnostic products
  • GMP-grade products
  • Application-specific PCR
  • Other PCR-related products
  • PCR thermal cyclers
  • Cloning
  • In-Fusion Cloning
  • Competent cells
  • Ligation kits
  • Mutagenesis kits
  • Ligation enzymes
  • Restriction enzymes
  • Modifying enzymes
  • X-Gal and IPTG
  • Linkers, primers, and cloning vectors
  • Agarose gel electrophoresis
  • Nucleic acid extraction
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • RNA cleanup kits
  • Viral DNA and RNA purification kits
  • Accessories and components
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Secondary antibodies
  • Antibody and ELISA accessories
  • Fluorescent protein antibodies
  • Cell biology assays
  • Extracellular vesicle isolation
  • Exosome isolation (cell culture)
  • Reporter systems
  • Apoptosis detection kits
  • Epigenetics
  • Cell biology reagents
  • RNA interference
  • Cell-culture accessories
  • Signal transduction
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • Real-time PCR primer sets
  • References and standards for qPCR
  • RNA extraction and analysis for real-time qPCR
  • Application-specific qPCR
  • cDNA synthesis
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • cDNA synthesis accessories
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • Apollo library prep system introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Product line overview
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Newsletters
  • Webinars
  • Citations
  • Posters
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Stem cell research
  • Protocols
  • Applications
  • Technical notes
  • Posters
  • Webinars
  • Videos
  • FAQs
  • Citations
  • Selection guides
  • Overview
  • Protein research
  • Capturem technology
  • Antibody purification
  • His-tag purification
  • Other tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Expression systems
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • PCR FAQs
  • Go green with lyophilized enzymes
  • LA PCR technology
  • Cloning
  • In-Fusion Cloning: general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and tech notes
  • Sign up to stay updated
  • Traditional molecular cloning
  • Nucleic acid purification
  • Product finder
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • Parallel DNA, RNA & protein
  • Automated DNA and RNA purification
  • RNA purification
  • Hard-to-lyse samples
  • Antibodies and ELISA
  • Osteocalcin focus
  • Cell biology assays
  • Extracellular vesicle isolation
  • Technical notes
  • FAQs
  • Citations
  • Real-time PCR
  • Product finder
  • Reaction size guidelines for qPCR
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Overview
  • Technical notes
  • FAQs
  • cDNA synthesis
  • Premium total and poly A+ RNA
  • SMARTer RACE 5'/3' Kit—advances in SMARTer PCR cDNA synthesis
  • Cloning antibody variable regions
  • Services & Support
  • Instrument services
  • OEM & custom enzyme manufacturing
  • Stem cell services
  • Gene and cell therapy manufacturing services
  • Customer service
  • Technical support
  • Sales
  • Shipping & delivery
  • Feedback
  • Corporate development
  • Webinars from Takara Bio
  • Vector information
  • Online tools
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Corporate development
  • Partnering & OEM solutions
  • In licensing
  • Out licensing
  • Submit a licensing request
  • Webinars from Takara Bio
  • NGS: biomarkers and oncology
  • NGS: immunology
  • Stem cells
  • Real-time PCR
  • Gene function
  • Protein science
  • Vector information
  • Vector document overview
  • Vector document finder
  • Online tools
  • GoStix Plus FAQs
  • Areas of interest
  • Pathogen detection
  • Vaccine development
  • Cancer research
  • Immunotherapy research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • DSS Takara Bio India Pvt. Ltd : Manufacturing
  • Our partners
  • Special offers
  • New products
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Calendar
  • Conferences
  • Speak with us
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Special offers
  • RT-qPCR bundle promotion
  • GoStix Plus special offers
  • PCR samples
  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About