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Overviews DNA purification from stool
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Product overview

NucleoSpin RNA Stool

The NucleoSpin RNA Stool kit is designed for the efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit combines a chemical disruption of the stool sample using NucleoZol and Lysis Buffer RST1 with a mechanical lysis using MN Bead Tubes Type A (which contain ceramic beads). No protease digestion is required.

  • Combination of mechanical homogenization and chemical lysis ensures high RNA recovery, including small RNA
  • Effective removal of PCR inhibitors
  • Suitable for various human and animal stool samples
At-a-glance Bead tube disruption High yields qRT-PCR performance

At-a-glance  

Technology Silica membrane technology combined with MN Bead Tubes Type A
Format Mini spin columns
Sample material Fresh or frozen stool samples
Fragment size ≥18 nt
Typical yield  10–30 µg
A260/A280 1.9–2.1
A260/A230 1.8–2.5
Typical RIN
(RNA integrity number)
>7.5
Elution volume 100 µl
Preparation time 70 min/10 preps
Binding capacity 200 µg
NucleoSpin RNA Stool workflow

Figure 1. The NucleoSpin RNA Stool procedure.

Bead tube disruption  

NucleoSpin RNA Stool includes NucleoSpin Bead Tubes Type A

Figure 2. The NucleoSpin RNA Stool kit includes MN Bead Tubes Type A. The NucleoSpin RNA Stool kit utilizes MN Bead Tubes Type A (Panel A), 0.6–0.8-mm ceramic beads included in the kit, for efficient mechanical disruption of stool samples. The beads are designed to be used in conjunction with the MN Bead Tube Holder (Cat. No. 740469) on a Vortex-Genie 2 (Panel B) or with a mixer mill (see below). MN Bead Tubes Type A are also available separately.

High yields  

Higher yields with NucleoSpin RNA Stool

Figure 3. NucleoSpin RNA Stool provides higher yields than competitor kits. Two human stool samples were processed in triplicates using 250 mg of stool sample with two competitor kits (Q and Z) and only 200 mg of stool sample with NucleoSpin (MN), all according to their standard protocols. The Competitor Q protocol that was performed included an optional phenol-based lysis step. Each eluate was analyzed using UV spectroscopy to determine RNA yield. The samples processed using NucleoSpin RNA Stool showed the highest RNA yield of all the human stool samples.

qRT-PCR performance  

Better qPCR performance with NucleoSpin RNA Stool

Figure 4. NucleoSpin RNA Stool provides better qPCR performance than competitor kits. The NucleoSpin RNA Stool samples and the Q samples were each eluted in 100 μl, while the Z eluates were adjusted to 100 μl to obtain comparable RNA concentrations. 1 μl of eluate was used for qRT-PCR of a 466-bp amplicon on a Roche LightCycler using a one-step qRT-PCR kit. NucleoSpin RNA Stool (MN) showed better qRT-PCR performance than the two competitor kits (Q and Z).

Related Products

Cat. # Product Size License Quantity Details
740130.10 NucleoSpin® RNA Stool 10 Preps USD $132.00

The NucleoSpin RNA Stool kit is designed for efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit employs silica-membrane technology in a mini spin column format, and typically yields 10–30 µg of RNA from 200-mg inputs of fresh or frozen stool. The kit workflow allows for processing of 10 samples in parallel in approximately 70 min.

Cat. # 740130.10 includes sufficient quantities of reagents and materials for processing of 10 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin RNA Stool procedure

NucleoSpin RNA Stool procedure

NucleoSpin RNA Stool procedure.

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Highest yield with NucleoSpin RNA Stool

Highest yield with NucleoSpin RNA Stool

Highest yield with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, extractions were performed in triplicate. For each sample, NucleoSpin RNA Stool provided 20–60% higher yields compared to the competitor kits.

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Highest RNA purity and integrity with NucleoSpin RNA Stool

Highest RNA purity and integrity with NucleoSpin RNA Stool

Highest RNA purity and integrity with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, four extractions were performed. For each sample, NucleoSpin RNA Stool yielded RNA of the highest purity (A260/A280, top) and integrity (RIN, bottom) compared to the competitor kits.

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NucleoSpin RNA Stool shows best performance in qPCR

NucleoSpin RNA Stool shows best performance in qPCR

NucleoSpin RNA Stool shows best performance in qPCR. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For samples processed with NucleoSpin RNA Stool and protocol "Q", RNA was eluted in 100-µl volumes, whereas eluates from protocol "Z" were each adjusted to a final volume of 100 µl to obtain comparable RNA concentrations for each protocol. For each sample and protocol, extractions were performed in triplicate. For each sample, RNA obtained with NucleoSpin RNA Stool provided better performance in qPCR compared to RNA prepared with the competitor kits.

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740130.10: NucleoSpin RNA Stool

740130.10: NucleoSpin RNA Stool
740130.50 NucleoSpin® RNA Stool 50 Preps USD $497.00

The NucleoSpin RNA Stool kit is designed for efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit employs silica-membrane technology in a mini spin column format, and typically yields 10–30 µg of RNA from 200-mg inputs of fresh or frozen stool. The kit workflow allows for processing of 10 samples in parallel in approximately 70 min.

Cat. # 740130.50 includes sufficient quantities of reagents and materials for processing of 50 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

NucleoSpin RNA Stool procedure

NucleoSpin RNA Stool procedure

NucleoSpin RNA Stool procedure.

Back

Highest yield with NucleoSpin RNA Stool

Highest yield with NucleoSpin RNA Stool

Highest yield with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, extractions were performed in triplicate. For each sample, NucleoSpin RNA Stool provided 20–60% higher yields compared to the competitor kits.

Back

Highest RNA purity and integrity with NucleoSpin RNA Stool

Highest RNA purity and integrity with NucleoSpin RNA Stool

Highest RNA purity and integrity with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, four extractions were performed. For each sample, NucleoSpin RNA Stool yielded RNA of the highest purity (A260/A280, top) and integrity (RIN, bottom) compared to the competitor kits.

Back

NucleoSpin RNA Stool shows best performance in qPCR

NucleoSpin RNA Stool shows best performance in qPCR

NucleoSpin RNA Stool shows best performance in qPCR. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For samples processed with NucleoSpin RNA Stool and protocol "Q", RNA was eluted in 100-µl volumes, whereas eluates from protocol "Z" were each adjusted to a final volume of 100 µl to obtain comparable RNA concentrations for each protocol. For each sample and protocol, extractions were performed in triplicate. For each sample, RNA obtained with NucleoSpin RNA Stool provided better performance in qPCR compared to RNA prepared with the competitor kits.

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740130.50: NucleoSpin RNA Stool

740130.50: NucleoSpin RNA Stool
740786.50 MN Bead Tubes Type A 50 Preps USD $125.00

Pack of fifty 2-ml tubes containing ceramic beads for sample homogenization. Use in combination with the MN Bead Tube Holder (Cat. # 740469) and NucleoSpin Soil (e.g., Cat. # 740780.50) or Nucleospin Stool (Cat. # 740472.50), miniprep kits for isolation of total DNA from soil, sediments, or stool samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740786.50: NucleoSpin Bead Tubes Type A

740786.50: NucleoSpin Bead Tubes Type A

Required Products

Cat. # Product Size Price License Quantity Details
740469 MN Bead Tube Holder Each USD $41.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

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740469: MN Bead Tube Holder

740469: MN Bead Tube Holder
740404.200 NucleoZOL™ 200 mL USD $321.00

NucleoZOL Reagent for the isolation of small and large RNA. (Bottle of 200 ml)

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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The NucleoZOL RNA isolation procedure

The NucleoZOL RNA isolation procedure
The NucleoZOL RNA isolation procedure.

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Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor

Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor
Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor. Total RNA was isolated from 50 mg rat lung tissue using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. DNA contamination was quantified by qPCR (competitor’s ZOL=100%). A minimal amount of DNA is carried over during purification with NucleoZOL, compared to the competitor’s ZOL.

Back

NucleoZOL provides similar or better RNA yields than a leading competitor

NucleoZOL provides similar or better RNA yields than a leading competitor
NucleoZOL provides similar or better RNA yields than a leading competitor. RNA was extracted from different starting materials using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. In each case, RNA was quantified by qRT-PCR and relative yields were calculated (NucleoZOL=100%). RNA isolation with NucleoZOL results in similar or better RNA yields than the competitor’s ZOL.

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No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor. Total RNA was isolated from 50 mg rat lung tissue using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. The A260/A230 absorption ratio of total RNA isolated using NucleoZOL indicates excellent purity. The lower A260/A230 ratio of total RNA isolated using the competitor’s ZOL indicates a phenol carryover.

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740404.200: NucleoZOL

740404.200: NucleoZOL
740469 MN Bead Tube Holder Each USD $41.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740469: MN Bead Tube Holder

740469: MN Bead Tube Holder

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