NucleoSpin RNA Stool

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Nucleic acid purification

Product overview

The NucleoSpin RNA Stool kit is designed for the efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit combines a chemical disruption of the stool sample using NucleoZol and Lysis Buffer RST1 with a mechanical lysis using MN Bead Tubes Type A (which contain ceramic beads). No protease digestion is required.

Combination of mechanical homogenization and chemical lysis ensures high RNA recovery, including small RNA
Effective removal of PCR inhibitors
Suitable for various human and animal stool samples

At-a-glance Bead tube disruption High yields qRT-PCR performance

At-a-glance

Technology	Silica membrane technology combined with MN Bead Tubes Type A
Format	Mini spin columns
Sample material	Fresh or frozen stool samples
Fragment size	≥18 nt
Typical yield	10–30 µg
A₂₆₀/A₂₈₀	1.9–2.1
A₂₆₀/A₂₃₀	1.8–2.5
Typical RIN (RNA integrity number)	>7.5
Elution volume	100 µl
Preparation time	70 min/10 preps
Binding capacity	200 µg

Bead tube disruption

NucleoSpin RNA Stool includes NucleoSpin Bead Tubes Type A

High yields

qRT-PCR performance

Better qPCR performance with NucleoSpin RNA Stool

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The NucleoSpin RNA Stool kit is designed for efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit employs silica-membrane technology in a mini spin column format, and typically yields 10–30 µg of RNA from 200-mg inputs of fresh or frozen stool. The kit workflow allows for processing of 10 samples in parallel in approximately 70 min. Cat. # 740130.10 includes sufficient quantities of reagents and materials for processing of 10 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740130.10: NucleoSpin RNA Stool Back NucleoSpin RNA Stool procedure NucleoSpin RNA Stool procedure. Back Highest yield with NucleoSpin RNA Stool Highest yield with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, extractions were performed in triplicate. For each sample, NucleoSpin RNA Stool provided 20–60% higher yields compared to the competitor kits. Back Highest RNA purity and integrity with NucleoSpin RNA Stool Highest RNA purity and integrity with NucleoSpin RNA Stool. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For each sample and protocol, four extractions were performed. For each sample, NucleoSpin RNA Stool yielded RNA of the highest purity (A₂₆₀/A₂₈₀, top) and integrity (RIN, bottom) compared to the competitor kits. Back NucleoSpin RNA Stool shows best performance in qPCR NucleoSpin RNA Stool shows best performance in qPCR. Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool ("MN"), whereas 250-mg samples were processed with the competitor kits. For protocol "Q", the optional phenol-based lysis step was included. For samples processed with NucleoSpin RNA Stool and protocol "Q", RNA was eluted in 100-µl volumes, whereas eluates from protocol "Z" were each adjusted to a final volume of 100 µl to obtain comparable RNA concentrations for each protocol. For each sample and protocol, extractions were performed in triplicate. For each sample, RNA obtained with NucleoSpin RNA Stool provided better performance in qPCR compared to RNA prepared with the competitor kits.
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