| Technology | Silica-membrane technology combined with MN Bead Tubes Type D |
| Format | Mini spin columns |
| Starting material | <40 mg fresh, frozen, dried, or ethanol-preserved insect/crustacean samples |
| Fragment size | 200 bp–approx. 50 kb |
| Typical yield | <25 µg (varies by sample and disruption device) |
| A260/A280 | 1.7–1.9 |
| Elution volume | 25–200 µl |
| Preparation time | 35 min/6 prep |
| Binding capacity | 60 µg |
Product overview
NucleoSpin DNA Insect
The NucleoSpin DNA Insect kit is optimized for the purification of genomic DNA from fresh, frozen, dried, or ethanol-preserved insect or crustacean samples. The isolated DNA may be used for genotyping, sequencing, or any other common applications. The kit utilizes MN Bead Tubes Type D (steel beads; included in kit) in combination with a common disruption device, e.g., MN Bead Tube Holder, or a swing mill in order to lyse different cell types and tissues using a single procedure. Additional MN bead tubes can be ordered separately if needed.
At-a-glance
Bead tube disruption
Figure 1. The NucleoSpin DNA Insect kit includes MN Bead Tubes Type D. The NucleoSpin DNA Insect kit utilizes MN Bead Tubes Type D (Panel A), 3-mm steel beads included in the kit, for efficient mechanical disruption of insect samples. The beads are designed to be used in conjunction with the MN Bead Tube Holder on a Vortex Genie (Panel B), or with a mixer mill, in order to lyse different cell types and tissues using a single procedure. MN Bead Tubes Type D are also available separately.
High yield
Figure 2. Excellent DNA recovery even from small samples. Genomic DNA was isolated from Drosophila melanogaster using the NucleoSpin DNA Insect kit in comparison with two competitor kits, according to the standard procedure. Panel A. DNA yield measured by qPCR showed highly efficient recovery using the NucleoSpin DNA Insect kit, even from small amounts of material. Panel B. The A260/A280 ratio was calculated to assess the purity of the isolated DNA. The optimal value of "1.8" is marked by a red line. DNA isolated with the NucleoSpin DNA Insect kit was consistently pure.
Higher quality
Figure 3. Superior yield and quality of DNA from different insect species. Panel A. DNA from different species was isolated with the NucleoSpin DNA Insect kit and separated by agarose gel electrophoresis. Lane 1: Fruit fly. Lane 2: Mosquito larvae. Lane 3: Field cricket. Lane. 4: House cricket. Lane 5: Mealworm. High molecular weight DNA was observed in all samples. Panel B. DNA was isolated from a single fruit fly (D. melanogaster) with three different extraction methods. Intact and pure high molecular weight DNA was isolated with the NucleoSpin DNA Insect kit (MN). Extraction with competitor kits resulted in DNA degradation (Z) or RNA contamination (Q).
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