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Purify genomic DNA from fresh, frozen, dried or ethanol preserved insect or crustacean samples. NucleoSpin DNA Insect product page
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Home › Learning centers › Nucleic acid purification › Genomic DNA purification › Other organisms and samples › NucleoSpin DNA Insect

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Purify genomic DNA from fresh, frozen, dried or ethanol preserved insect or crustacean samples. NucleoSpin DNA Insect product page
Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Product overview

NucleoSpin DNA Insect

The NucleoSpin DNA Insect kit is optimized for the purification of genomic DNA from fresh, frozen, dried, or ethanol-preserved insect or crustacean samples. The isolated DNA may be used for genotyping, sequencing, or any other common applications. The kit utilizes MN Bead Tubes Type D (steel beads; included in kit) in combination with a common disruption device, e.g., MN Bead Tube Holder, or a swing mill in order to lyse different cell types and tissues using a single procedure. Additional MN bead tubes can be ordered separately if needed.

At-a-glance Bead tube disruption High yield Higher quality

At-a-glance  

Technology Silica-membrane technology combined with MN Bead Tubes Type D
Format Mini spin columns
Starting material <40 mg fresh, frozen, dried, or ethanol-preserved insect/crustacean samples
Fragment size 200 bp–approx. 50 kb
Typical yield <25 µg (varies by sample and disruption device)
A260/A280 1.7–1.9
Elution volume 25–200 µl
Preparation time 35 min/6 prep
Binding capacity 60 µg

Bead tube disruption  

NucleoSpin Bead Tubes Type D are included

Figure 1. The NucleoSpin DNA Insect kit includes MN Bead Tubes Type D. The NucleoSpin DNA Insect kit utilizes MN Bead Tubes Type D (Panel A), 3-mm steel beads included in the kit, for efficient mechanical disruption of insect samples. The beads are designed to be used in conjunction with the MN Bead Tube Holder on a Vortex Genie (Panel B), or with a mixer mill, in order to lyse different cell types and tissues using a single procedure. MN Bead Tubes Type D are also available separately.

High yield  

DNA insect Recovery

Figure 2. Excellent DNA recovery even from small samples. Genomic DNA was isolated from Drosophila melanogaster using the NucleoSpin DNA Insect kit in comparison with two competitor kits, according to the standard procedure. Panel A. DNA yield measured by qPCR showed highly efficient recovery using the NucleoSpin DNA Insect kit, even from small amounts of material. Panel B. The A260/A280 ratio was calculated to assess the purity of the isolated DNA. The optimal value of "1.8" is marked by a red line. DNA isolated with the NucleoSpin DNA Insect kit was consistently pure.

Higher quality  

Yield and quality of DNA from different insect specie

Figure 3. Superior yield and quality of DNA from different insect species. Panel A. DNA from different species was isolated with the NucleoSpin DNA Insect kit and separated by agarose gel electrophoresis. Lane 1: Fruit fly. Lane 2: Mosquito larvae. Lane 3: Field cricket. Lane. 4: House cricket. Lane 5: Mealworm. High molecular weight DNA was observed in all samples. Panel B. DNA was isolated from a single fruit fly (D. melanogaster) with three different extraction methods. Intact and pure high molecular weight DNA was isolated with the NucleoSpin DNA Insect kit (MN). Extraction with competitor kits resulted in DNA degradation (Z) or RNA contamination (Q).

Related Products

Cat. # Product Size Price License Quantity Details
740470.50 NucleoSpin® DNA Insect 50 Preps USD $255.00

Mini prep kit (50 preps) for the purification of genomic DNA from fresh, frozen, dried or ethanol preserved insect or crustacean samples. The isolated DNA is used for genotyping, sequencing or any other common application. The NucleoSpin DNA Insect kit utilizes MN Bead Tubes Type D (steel beads; included in kit) in combination with a common disruption device, e.g., MN Bead Tube Holder, or a swing mill in order to lyse different cell types and tissues within a single procedure.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740470.50: NucleoSpin DNA Insect

740470.50: NucleoSpin DNA Insect

Required Products

Cat. # Product Size Price License Quantity Details
740469 MN Bead Tube Holder Each USD $44.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

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740469: MN Bead Tube Holder

740469: MN Bead Tube Holder
740469 MN Bead Tube Holder Each USD $44.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740469: MN Bead Tube Holder

740469: MN Bead Tube Holder
740814.50 MN Bead Tubes Type D 50 Preps USD $135.00

MN Bead Tubes are 2-ml screw cap plastic tubes containing different types of beads depending on the application. They are intended for the disruption of biological samples and subsequent nucleic acid purification. MN Bead Tubes Type D consists of 3-mm steel beads for use with insect samples using the NucleoSpin DNA Insect kits or NucleoSpin Lipid Tissue kits. The beads should be used in combination with the MN Bead Tube Holder (Cat. # 740469) and an appropriate vortexer (e.g., Vortex-Genie 2 from Scientific Industries), or with a mixer mill (e.g., from Retsch GmbH).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740814.50: NucleoSpin Bead Tubes Type D

740814.50: NucleoSpin Bead Tubes Type D
740470.10 NucleoSpin® DNA Insect 10 Preps USD $66.00

Miniprep kit (10 preps) for the purification of genomic DNA from fresh, frozen, dried or ethanol-preserved insect or crustacean samples. The isolated DNA is used for genotyping, sequencing or any other common application. The NucleoSpin DNA Insect kit utilizes MN Bead Tubes Type D (steel beads; included in kit) in combination with a common disruption device, e.g., an MN Bead Tube Holder or a swing mill, in order to lyse different cell types and tissues within a single procedure. MN Bead Tubes Type D are also available separately (Cat. # 740814.50).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740470.10: NucleoSpin DNA Insect

740470.10: NucleoSpin DNA Insect

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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