Dissolution of paraffin and lysis of tissue samples are accomplished simultaneously with the Paraffin Dissolver. Following tissue solubilization and decrosslinking via Proteinase K digestion, DNA is bound to NucleoMag paramagnetic beads under appropriate buffer conditions, washed with two separate wash buffers to remove contaminants and salts, and high-purity genomic DNA is eluted under low-ionic-strength conditions (Figure 1).
Genomic DNA purification
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NucleoMag DNA FFPE
The NucleoMag DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples. Paraffin is first removed from the sample using Paraffin Dissolver. Tissue samples are then solubilized using a Proteinase K digestion to release DNA into solution. Nucleic acids are then bound to the paramagnetic beads via addition of NucleoMag beads and binding buffer to the lysate. After magnetic separation the NucleoMag beads are washed twice using two different wash buffers to remove contaminants and salts. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.
- Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
- Isolation of DNA from fresh and archived FFPE samples
- Isolation of DNA from specimen or object slides
- DNA can be used in downstream PCR applications
- Support protocol for RNA extraction from FFPE samples also available
Superior and consistent DNA yield
Genomic DNA was purified from formalin-fixed, paraffin-embedded mouse lung, liver, and kidney tissue using NucleoMag DNA FFPE (MN) and two competitor kits (A, I) (Figure 2). The NucleoMag DNA FFPE kit achieved higher DNA yields compared to competitors' kits.
Superb PCR performance
Genomic DNA was purified with NucleoMag DNA FFPE and two competitors' kits, then analyzed with LightCycler PCR and the DyNAmo Capillary SYBR Green qPCR Kit (amplicon size 191 bp) (Figure 3). Competitor A (Panel A) and Competitor I (Panel B) samples show higher CT values compared to NucleoMag DNA FFPE samples, indicating a lower DNA yield from competitors' kits.
Genomic DNA purification articles
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NucleoSpin Tissue XS
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NucleoMag 384 Plant
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NucleoSpin DNA Plasma Midi
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