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Genomic DNA purification

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Product overview

NucleoMag Blood

The NucleoMag Blood 200 µL kit (96-well format) and NucleoMag Blood 3 mL kit (24-well format) are designed for the rapid parallel purification of genomic DNA from whole blood. After lysis of the blood cells, genomic DNA is selectively bound to NucleoMag B-Beads. Contaminants are washed away, and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. NucleoMag technology can easily be adapted to common liquid handling instruments. It can be used with either static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.

  • Single-tube procedure minimizes risk of cross-contamination
  • Small elution volumes: ≥50 µl for NucleoMag Blood 200 µL; ≥1 ml for NucleoMag Blood 3 mL
  • Complete processing is possible at room temperature
  • Easily adapted to automated use
  • Consistently high yields

At a glance  

NucleoMag Blood 200 µL (Cat. # 744501.1 and 744501.4)
 NucleoMag Blood 3 mL (Cat. # 744502.1)
Technology Magnetic bead technology Magnetic bead technology
Format Highly reactive superparamagnetic beads Highly reactive superparamagnetic beads
Processing Manual or automated Automated*
Starting material 200 µl whole blood (fresh or frozen, EDTA or citrate treated) 3 ml whole blood (fresh or frozen, EDTA or citrate treated)
Fragment size 300 bp to ~50 kb 300 bp to ~50 kb
Typical yield  2–8 µg (from 200 µl of blood) 100–130 µg (from 3 ml of blood)
A260/280 1.6–1.9 1.6–1.9
Elution volume 50–1,000 µl 1 ml
Preparation time 45 min/96 preps* 60 min/24 preps*
Binding capacity Approx. 0.4 µg/µl Approx. 0.4 µg/µl

*Established on KingFisher Flex.

Highly reproducible DNA yields  

Isolation of DNA from fresh or frozen 200-µl human blood samples using the NucleoMag Blood 200 µL kit provided highly reproducible yields (Figure 1).

Highly reproductible DNA yields from 200 µl human blood samples

Figure 1. Highly reproductible DNA yields from fresh or frozen human blood samples using the NucleoMag Blood 200 µL kit. Samples were taken from six different donors (#1–#6). Aliquots from each sample were purified directly (fresh), or after storage at –20°C for one day (frozen). For each aliquot, eight extractions were performed, and the indicated value represents the median yield (µg) for all eight extractions.

Isolation of high-integrity DNA  

The NucleoMag Blood 3 mL kit was used to extract DNA of high integrity from blood samples obtained from 24 individual donors, as shown by agarose gel analysis (Figure 2).

Isolation of high-integrity DNA from human blood

Figure 2. Isolation of high-integrity DNA from human blood using the NucleoMag Blood 3 mL kit. DNA from 24 individual human blood donors (Lanes 1–24) was extracted in duplicate and analyzed on a 0.7 % (w/v) TAE agarose gel stained with ethidium bromide. Lane L: λHindIII size marker. The gDNA migrates as a distinct band with a molecular weight >23.5 kb, indicating that it is of high integrity.

High yields of high-purity DNA  

High yields of highly pure DNA were obtained from a pool of blood samples extracted using the NucleoMag Blood 3 mL kit (Figure 3).

High-yield, high-purity DNA from 3-ml human blood samples

Figure 3. High yields of highly pure DNA were isolated from 3-ml human blood samples using the NucleoMag Blood 3 mL kit. 24 replicates of 3 ml each were extracted using the NucleoMag Blood 3 mL kit.

Protocol  

The NucleoMag Blood 200 µL procedure

Figure 4. The NucleoMag Blood 200 µL procedure. NucleoMag technology can easily be adapted to common liquid handling instruments using either static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.

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