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Home › Learning centers › Nucleic acid purification › Genomic DNA purification › Tissue › NucleoSpin Tissue

Genomic DNA purification

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Product overview

NucleoSpin Tissue

  • High DNA recovery and purity
  • Reliable DNA purification for reproducible results
  • Over 16 optimized support protocols for a wide variety of starting materials
  • Also available for QIAcube (contact technical support)

NucleoSpin Tissue kits are designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, forensic samples (hair, dried blood spots, buccal swabs, cigarette filters, etc.), and clinical samples (stool, urine).

At a glance Sample types Data: aged samples Data: clinical samples Data: veterinary testing

At a glance  

Technology Silica membrane
Format Mini spin columns
Sample material 1–25 mg tissue; 102–107 cells
Fragment size 200 bp to >30 kbp
Typial yield 20–35 μg
Binding capacity 60 μg
Typical Ratio A260/A280 1.7–1.9
Elution volume 60–100 μl
Preparation time ~20 min/prep (excluding lysis)

Sample types  

NucleoSpin Tissue shows proven reliability in DNA purification from different sample materials. The following table presents a selection of peer-reviewed publications citing NucleoSpin Tissue.

Sample material DNA application Publication
Dried blood spots on newborn screening cards DNA virus detection by PCR C. S. Gibson et al., BMJ 332 (2006)
Buccal swabs PCR of Y-chromosomal STR loci H. Rodig et al., Int J Legal Med. 121 (2007)
Mice ear markings PCR of Car9 and Car2 gene targets P. Pan et al., J. Physiol. 571 (2006)
Cells (ciliate Oxytricha trifallax) PCR M. Nowacki et al., Nature 451 (2007)
FFPE (formaline-fixed paraffin-embedded) tissue Determination of the methylation status of a promoter R. Schneider-Stock et al., J. Clin. Oncol. 21 (2003)
Ants (ethanol preserved) PCR, target: mitochondrial DNA, cytochrome oxidase R. Savolainen and K. Vepsäläinen, PNAS 100 (2003)
Rat tails and embryonic tissue Genotyping to distinguish between wild type and aralar-deficient animals B. Pardo et al., J. Biol. Chem. 281 (2006)
Microdissected frozen and/or paraffin-embedded tissue Mutation analysis using PCR and automated sequencing V. Máximo et al., British Journal of Cancer 92 (2005)
Malignant melanoma  Array—CGH and mutation analysis  G. Jönsson et al., Oncogene 26 (2007)
Wasp leg, small pieces (1 mm long) PCR, a 658-bp target, near the 5' terminus of the CO1 gene M. A. Smith et al., PNAS 105 (2008)
Cells, parental and lentivirally transduced  PCR, Target: HSV-TkEGFP  R. Uch et al., Cancer Gene Therapy 10, 2003

Data: aged samples  

 Isolation of DNA from aged tissue samples

Isolation of DNA from aged tissue samples (wood mouse, Apodemus sylvaticus). Ethanol-preserved samples of wood mouse tissue (25 mg) were subjected to DNA isolation with NucleoSpin Tissue following the standard protocol. The ages of the samples were 1 year (Sample 1), 44 years (Sample 2), and 102 years (Sample 3). Gel electrophoresis shows that genomic DNA was successfully isolated from all three specimens. High-molecular-weight DNA was isolated from Sample 1. DNA fragments in Samples 2 and 3 are shorter due to the age of the samples. Yield and purity of DNA were measured with NanoDrop.

Data kindly provided by Dr. Tiemann, Laboratory Prof. Hagedorn, Herford, Germany

Data: clinical samples  

This gel shows PCR results of DNA purified from urine, liquor, feces, mother's milk, and plasma with NucleoSpin Tissue. CMV can be detected in mother's milk as well as in several samples from the child (marked with arrows). The other samples do not carry the virus.

Detection of CMV virus

Detection of CMV in different clinical samples. Cytomegalovirus (CMV) belongs to the family of Herpesviridae that contains large double-stranded DNA genomes. The virus is widely spread and transferred by direct contact. The detection of CMV from different matrices requires sensitive purification of genomic DNA as well as effective removal of PCR inhibitors for a successful subsequent DNA amplification.

Data kindly provided by Dr. Tiemann, Laboratory Prof. Hagedorn, Herford, Germany

Data: veterinary testing  

 Isolation of genomic DNA from koi

Isolation of genomic DNA from koi (Cyprius carpio) and amplification in real-time TaqMan PCR. NucleoSpin Tissue was used to isolate DNA from samples of three Koi gills. Koi glucokinase was amplified in real-time TaqMan PCR, following the protocol described by Gilad et al. Diseases of Aquatic Organisms. 60 (2004). As indicated in the graph, all three samples were amplified successfully.

Data kindly provided by the Staatliches Veterinäruntersuchungsamt (Governmental Veterinary Testing Department) Arnsberg, Germany

Ordering Information

Cat. # Product Size License Quantity Details
740952.50 NucleoSpin® Tissue 50 Preps USD $142.00

NucleoSpin Tissue (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections.

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The NucleoSpin Tissue procedure

The NucleoSpin Tissue procedure

The NucleoSpin Tissue procedure.

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740952.50: NucleoSpin Tissue

740952.50: NucleoSpin Tissue
740952.250S NucleoSpin® Tissue Columns 250 Columns USD $304.00

NucleoSpin Tissue Columns are mini spin columns intended for use with the NucleoSpin Tissue kits for the isolation of genomic DNA from a wide array of cells and tissues.

Cat. # 740952.250S consists of a pack of 250 columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections.

Back

The NucleoSpin Tissue procedure

The NucleoSpin Tissue procedure

The NucleoSpin Tissue procedure.

Back

740952.250S: NucleoSpin Tissue Columns

740952.250S: NucleoSpin Tissue Columns
740952.250 NucleoSpin® Tissue 250 Preps USD $610.00

NucleoSpin Tissue (250) 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections.

Back

The NucleoSpin Tissue procedure

The NucleoSpin Tissue procedure

The NucleoSpin Tissue procedure.

Back

740952.250: NucleoSpin Tissue

740952.250: NucleoSpin Tissue
740952.240C NucleoSpin® Tissue 240 Preps USD $610.00

Kit for use on QIAcube. NucleoSpin Tissue (240) 240 preps for the isolation of genomic DNA from tissue Buffers, Proteinase K, NucleoSpin Tissue Columns

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

740952.240C: NucleoSpin Tissue

740952.240C: NucleoSpin Tissue
740952.10 NucleoSpin® Tissue Each USD $32.00

NucleoSpin Tissue (10) 10 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

740952.10: NucleoSpin Tissue

740952.10: NucleoSpin Tissue


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