| Technology | Silica membrane |
| Format | Mini spin columns |
| Sample material | 1–25 mg tissue; 102–107 cells |
| Fragment size | 200 bp to >30 kbp |
| Typial yield | 20–35 μg |
| Binding capacity | 60 μg |
| Typical Ratio A260/A280 | 1.7–1.9 |
| Elution volume | 60–100 μl |
| Preparation time | ~20 min/prep (excluding lysis) |
Product overview
NucleoSpin Tissue
- High DNA recovery and purity
- Reliable DNA purification for reproducible results
- Over 16 optimized support protocols for a wide variety of starting materials
- Also available for QIAcube (contact technical support)
NucleoSpin Tissue kits are designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, forensic samples (hair, dried blood spots, buccal swabs, cigarette filters, etc.), and clinical samples (stool, urine).
At a glance
Sample types
NucleoSpin Tissue shows proven reliability in DNA purification from different sample materials. The following table presents a selection of peer-reviewed publications citing NucleoSpin Tissue.
| Sample material | DNA application | Publication |
| Dried blood spots on newborn screening cards | DNA virus detection by PCR | C. S. Gibson et al., BMJ 332 (2006) |
| Buccal swabs | PCR of Y-chromosomal STR loci | H. Rodig et al., Int J Legal Med. 121 (2007) |
| Mice ear markings | PCR of Car9 and Car2 gene targets | P. Pan et al., J. Physiol. 571 (2006) |
| Cells (ciliate Oxytricha trifallax) | PCR | M. Nowacki et al., Nature 451 (2007) |
| FFPE (formaline-fixed paraffin-embedded) tissue | Determination of the methylation status of a promoter | R. Schneider-Stock et al., J. Clin. Oncol. 21 (2003) |
| Ants (ethanol preserved) | PCR, target: mitochondrial DNA, cytochrome oxidase | R. Savolainen and K. Vepsäläinen, PNAS 100 (2003) |
| Rat tails and embryonic tissue | Genotyping to distinguish between wild type and aralar-deficient animals | B. Pardo et al., J. Biol. Chem. 281 (2006) |
| Microdissected frozen and/or paraffin-embedded tissue | Mutation analysis using PCR and automated sequencing | V. Máximo et al., British Journal of Cancer 92 (2005) |
| Malignant melanoma | Array—CGH and mutation analysis | G. Jönsson et al., Oncogene 26 (2007) |
| Wasp leg, small pieces (1 mm long) | PCR, a 658-bp target, near the 5' terminus of the CO1 gene | M. A. Smith et al., PNAS 105 (2008) |
| Cells, parental and lentivirally transduced | PCR, Target: HSV-TkEGFP | R. Uch et al., Cancer Gene Therapy 10, 2003 |
Data: aged samples
Isolation of DNA from aged tissue samples (wood mouse, Apodemus sylvaticus). Ethanol-preserved samples of wood mouse tissue (25 mg) were subjected to DNA isolation with NucleoSpin Tissue following the standard protocol. The ages of the samples were 1 year (Sample 1), 44 years (Sample 2), and 102 years (Sample 3). Gel electrophoresis shows that genomic DNA was successfully isolated from all three specimens. High-molecular-weight DNA was isolated from Sample 1. DNA fragments in Samples 2 and 3 are shorter due to the age of the samples. Yield and purity of DNA were measured with NanoDrop.
Data: clinical samples
This gel shows PCR results of DNA purified from urine, liquor, feces, mother's milk, and plasma with NucleoSpin Tissue. CMV can be detected in mother's milk as well as in several samples from the child (marked with arrows). The other samples do not carry the virus.
Detection of CMV in different clinical samples. Cytomegalovirus (CMV) belongs to the family of Herpesviridae that contains large double-stranded DNA genomes. The virus is widely spread and transferred by direct contact. The detection of CMV from different matrices requires sensitive purification of genomic DNA as well as effective removal of PCR inhibitors for a successful subsequent DNA amplification.
Data: veterinary testing
Isolation of genomic DNA from koi (Cyprius carpio) and amplification in real-time TaqMan PCR. NucleoSpin Tissue was used to isolate DNA from samples of three Koi gills. Koi glucokinase was amplified in real-time TaqMan PCR, following the protocol described by Gilad et al. Diseases of Aquatic Organisms. 60 (2004). As indicated in the graph, all three samples were amplified successfully.
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