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NucleoMag DNA Food product page NucleoMag DNA Food product page
Extract and purifiy genomic DNA from many types of processed food or animal feed of plant or animal origin NucleoSpin Food product page
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NucleoMag DNA Food product page NucleoMag DNA Food product page
Extract and purifiy genomic DNA from many types of processed food or animal feed of plant or animal origin NucleoSpin Food product page
automated nucleic acid purification Automate your workflow

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Product overview

NucleoMag DNA Food

NucleoMag DNA Food is designed for the rapid manual and automated preparation of DNA from small-scale food or feed samples and can be used to detect the presence of GMO DNA or animal components in these samples.

  • Provided reagents can purify up to 200 mg of material with a typical yield of 0.1–10 μg of DNA (depending on individual samples)
  • Eluted DNA is ready for immediate use in subsequent reactions including real-time PCR, GMO detection, etc.
  • Designed for use with the NucleoMag SEP magnetic separator plate or other equivalent magnetic separation system
  • Time for the manual preparation of 96 samples is about 2 hours
  • Compatible with common automated liquid handling instruments and magnetic separators
At-a-glance Protocol DNA from many types of food Superior DNA yield

At-a-glance  

Technology Magnetic bead technology
Format Highly reactive superparamagnetic beads
Processing Manual or automated
Sample material <200 mg food or feed
Fragment size 300 bp—approx. 50 kb
Typical yield 0.1–10 μg
A260/A280 1.6–1.9
Elution volume 50–200 µl
Preparation time 120 min/96 preps (excluding lysis)
Binding capacity 0.4 µg/µl beads

Protocol  

Summary of protocol

Figure 1. Summary of protocol. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads under specialized buffer conditions. Sample lysis is achieved by incubation of samples with Proteinase K at 65 °C. Lysis mixtures are cleared by centrifugation or filtration in order to remove contaminants and residual cellular debris. Nucleic acids are then bound to the paramagnetic beads via addition of Binding Buffer CB and the NucleoMag B-Beads to the cleared lysate. After magnetic separation, the paramagnetic beads are washed to remove contaminants and salts using Wash Buffers CMW, CQW, and 80% ethanol. Residual ethanol from previous wash steps is removed by air drying. Next, highly purified DNA is eluted with low-salt Elution Buffer (CE) and can be used directly in downstream applications. The NucleoMag DNA Food kit can either be used manually or automated on standard liquid handling instruments and automated magnetic separators.

DNA from many types of food  

Figure 2. Effective isolation of DNA from various food samples. Sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. DNA presence within the eluates was determined using qPCR.

 
Category Tested sample material
Raw,
vegetable origin
Carrot, potato, soy, maize, canola, linseed, oat, rice, wheat, sunflower seed, grape, seeds (tomato, cucumber, eggplant, melon, pepper), animal food
Raw,
animal origin
Venison, pork
Processed,
vegetable origin
Agave nectar, oatmeal
Processed,
animal origin
Milk, cheese, honey, salami, meat sausage, liver sausage
Complex processed, vegetable origin Vegetable broth, chips, pastry, cocoa, fried onions, tea, spices, tofu, juice, cereal bar, bread
Complex processed, animal origin Tiramisu, fruit gum, licorice, chocolate, Nutella, noodles, baby food, oil, drippings

Table I. Overview of different sample types that have been successfully tested. Following DNA extraction with the NucleoMag DNA Food kit, the presence of DNA was tested by either qPCR or via agarose gel electrophoresis.

Superior DNA yield  

Higher DNA yield with NucleoMag DNA Food versus leading competitor

Figure 3. Higher DNA yield with NucleoMag DNA Food versus a leading competitor. DNA from a variety of food sources was isolated using either the NucleoMag DNA Food (MN) or a competitor's kit (P) and DNA was detected using qPCR. The NucleoMag DNA Food kit consistently demonstrated lower Ct values than the competitor's kit, indicating a superior DNA yield.

Related Products

Cat. # Product Size Price License Quantity Details
744945.1 NucleoMag® DNA Food 1 x 96 Preps USD $385.00

NucleoMag DNA Food is designed for rapid manual or automated small-scale isolation of genomic DNA from up to 200 mg of food or feed samples of plant or animal origin, with typical yields ranging from 0.1–10 μg of pure DNA. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Magnetic separation can be performed either manually using the NucleoMag SEP magnetic separator or on an automated platform using standard liquid handling instruments and magnetic separators. Manual preparation of 96 samples in parallel can be completed in approximately 2 hours using NucleoMag SEP. Automated preparation of 96 samples can be completed in less than 2 hours depending on the configuration of the automation platform and the separator being used.

Cat. # 744945.1 includes sufficient quantities of reagents and beads for processing of 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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744945.1: NucleoMag DNA Food

744945.1: NucleoMag DNA Food

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NucleoMag DNA Food procedure

NucleoMag DNA Food procedure

NucleoMag DNA Food procedure.

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NucleoMag DNA Food can be used to isolate DNA from various food sample types

NucleoMag DNA Food can be used to isolate DNA from various food sample types

NucleoMag DNA Food can be used to isolate DNA from various food sample types. With NucleoMag DNA Food, a wide variety of food samples can be used as inputs for DNA isolation. For each sample type included in the graph, sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. The presence of DNA in the resulting eluates was determined using qPCR.

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Product comparison demonstrates superior performance of NucleoMag DNA Food

Product comparison demonstrates superior performance of NucleoMag DNA Food

Product comparison demonstrates superior performance of NucleoMag DNA Food. DNA was extracted from each of the indicated sample types in parallel using NucleoMag DNA Food and competitor kit "P". For each extraction, sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. The presence of DNA in the resulting eluates was determined using qPCR. For each sample type processed, NucleoMag DNA Food provided superior performance relative to kit "P", as indicated by the lower CT values for the DNA samples obtained with NucleoMag DNA Food.

744945.4 NucleoMag® DNA Food 4 x 96 Preps USD $1310.00

NucleoMag DNA Food is designed for rapid manual or automated small-scale isolation of genomic DNA from up to 200 mg of food or feed samples of plant or animal origin, with typical yields ranging from 0.1–10 μg of pure DNA. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Magnetic separation can be performed either manually using the NucleoMag SEP magnetic separator or on an automated platform using standard liquid handling instruments and magnetic separators. Manual preparation of 96 samples in parallel can be completed in approximately 2 hours using NucleoMag SEP. Automated preparation of 96 samples can be completed in less than 2 hours depending on the configuration of the automation platform and the separator being used.

Cat. # 744945.4 includes sufficient quantities of reagents and beads for processing 4 x 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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NucleoMag DNA Food procedure

NucleoMag DNA Food procedure

NucleoMag DNA Food procedure.

Back

NucleoMag DNA Food can be used to isolate DNA from various food sample types

NucleoMag DNA Food can be used to isolate DNA from various food sample types

NucleoMag DNA Food can be used to isolate DNA from various food sample types. With NucleoMag DNA Food, a wide variety of food samples can be used as inputs for DNA isolation. For each sample type included in the graph, sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. The presence of DNA in the resulting eluates was determined using qPCR.

Back

Product comparison demonstrates superior performance of NucleoMag DNA Food

Product comparison demonstrates superior performance of NucleoMag DNA Food

Product comparison demonstrates superior performance of NucleoMag DNA Food. DNA was extracted from each of the indicated sample types in parallel using NucleoMag DNA Food and competitor kit "P". For each extraction, sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. The presence of DNA in the resulting eluates was determined using qPCR. For each sample type processed, NucleoMag DNA Food provided superior performance relative to kit "P", as indicated by the lower CT values for the DNA samples obtained with NucleoMag DNA Food.

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744945.4: NucleoMag DNA Food

744945.4: NucleoMag DNA Food
744900 NucleoMag® SEP Each USD $835.00

NucleoMag SEP magnetic separator, for use with 96-well plates

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoMag SEP: magnetic separator for use with 96-well plates

NucleoMag SEP: magnetic separator for use with 96-well plates

NucleoMag SEP: magnetic separator for use with 96-well plates.

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744900: NucleoMag SEP

744900: NucleoMag SEP
744903 NucleoMag® SEP 24 Each USD $835.00

The NucleoMag SEP 24 magnetic separator is designed for the rapid manual purification of nucleic acids using the NucleoMag kits. NucleoMag kits employ Macherey-Nagel's magnetic bead-based sample processing technology, and enable rapid purification of high-quality nucleic acids from multiple samples in parallel.

The NucleoMag SEP 24 (Cat. # 744903) is used with 24-well plates.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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744903: NucleoMag SEP 24

744903: NucleoMag SEP 24
744901 NucleoMag® SEP Mini Each USD $325.00

The NucleoMag SEP Mini magnetic separator is designed for the rapid manual purification of nucleic acids using the NucleoMag kits. NucleoMag kits employ Macherey-Nagel's magnetic bead-based sample processing technology, and enable rapid purification of high-quality nucleic acids from multiple samples in parallel.

The NucleoMag SEP Mini (Cat. # 744901) is used with 1.5 ml or 2-ml reaction tubes and carries 12 tube positions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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NucleoMag SEP Mini: magnetic separator for use with 1.5 ml or 2-ml tubes

NucleoMag SEP Mini: magnetic separator for use with 1.5 ml or 2-ml tubes

NucleoMag SEP Mini: magnetic separator for use with 1.5 ml or 2-ml tubes.

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744901: NucleoMag SEP Mini

744901: NucleoMag SEP Mini
744902 NucleoMag® SEP Maxi Each USD $306.00

The NucleoMag SEP Maxi magnetic separator is designed for the rapid manual purification of nucleic acids using the NucleoMag kits. NucleoMag kits employ Macherey-Nagel's magnetic bead-based sample processing technology, and enable rapid purification of high-quality nucleic acids from multiple samples in parallel.

The NucleoMag SEP Maxi (Cat. # 744902) is used with 50-ml tubes and carries 4 tube positions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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NucleoMag SEP Maxi: magnetic separator for use with 50-ml tubes

NucleoMag SEP Maxi: magnetic separator for use with 50-ml tubes

NucleoMag SEP Maxi: magnetic separator for use with 50-ml tubes.

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744902: NucleoMag SEP Maxi

744902: NucleoMag SEP Maxi

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