Available formats | |||
NucleoSpin Plant II |
NucleoSpin Plant II Midi |
NucleoSpin Plant II Maxi |
|
Technology | Silica-membrane technology | Silica-membrane technology | Silica-membrane technology |
Format | Mini spin columns | Midi spin columns | Maxi spin columns |
Sample material |
<100 mg of plant material (wet wt) |
<400 mg of plant material (wet wt) |
<1,500 mg of plant material (wet wt) |
Lysate clarification | NucleoSpin Filters | NucleoSpin Filters L | NucleoSpin Filters XL |
Fragment size | 50 bp–approx. 50 kbp | 50 bp–approx. 50 kbp | 50 bp–approx. 50 kbp |
Typical yield | 1–30 µg | 10–100 µg | 50–300 µg |
A260/A280 | 1.8–1.9 | 1.8–1.9 | 1.8–1.9 |
Elution volume | 50–100 µl | 200–400 µl | 1,000–2,000 µl |
Preparation time | 30 min/prep | 90 min/prep | 90 min/prep |
Binding capacity | 50 µg | 200 µg | 500 µg |
Product Overview
NucleoSpin Plant II
- Genomic DNA from plant and fungi with high yield and purity
- Two lysis buffers—optimal processing of any plant material
- NucleoSpin filters included for clarification of lysate
- Optimized buffer compositions and silica membrane for improved DNA binding
- RNase A included
- Process up to 100 mg (wet weight) or 20 mg (dry weight) of starting material
- Typical yields range from 1 to 30 μg of DNA (depending on the individual sample)
Second-generation kits for isolation of genomic DNA from plant material, fungi, and other biological samples
As plants are very heterogeneous and contain a lot of different metabolites like polyphenols, polysaccharides, or acidic components, NucleoSpin Plant II offers two different lysis procedures, based on CTAB and SDS lysis methods (with Lysis Buffer PL1 and PL2, respectively), for optimal processing of various samples. The silica membrane in each NucleoSpin Plant II column is optimized to improve DNA binding. NucleoSpin filters are included for convenient clarification of lysates. The eluted DNA is ready to use for subsequent reactions like PCR, restriction analysis, etc.
At a glance
Competitor comparison
Sample quality data
Yield and purity data
Tabular overview of yield and purity for various plant samples isolated using NucleoSpin Plant II Maxi or Competitor Q.
DNA yield (µg) | A260/A280 | A260/A280 | ||
Willow | MN Q |
46 11 |
1.76 1.32 |
1.91 0.46 |
Rose | MN Q |
24 7 |
1.73 1.21 |
1.62 0.43 |
Fir needle | MN Q |
44 20 |
1.76 1.47 |
1.95 0.60 |
Wheat | MN Q |
232 142 |
1.84 1.77 |
2.09 1.53 |
Sugar beet | MN Q |
32 23 |
1.82 1.61 |
1.65 0.69 |
Universal reagent for RNA isolation
NucleoZOL is a highly efficient RNA isolation reagent for extracting high-quality miRNA and total RNA from a variety of starting materials, including cells, tissues, and liquids of human or animal origin, as well as plants, yeast, bacteria, viruses, and other sources.
See the data RNA purification kitsTakara Bio USA, Inc.
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