| Available formats | |||
|
NucleoSpin Plant II
|
NucleoSpin Plant II Midi
|
NucleoSpin Plant II Maxi
|
|
| Technology | Silica-membrane technology | Silica-membrane technology | Silica-membrane technology |
| Format | Mini spin columns | Midi spin columns | Maxi spin columns |
| Sample material |
<100 mg of plant material (wet wt) |
<400 mg of plant material (wet wt) |
<1,500 mg of plant material (wet wt) |
| Lysate clarification | NucleoSpin Filters | NucleoSpin Filters L | NucleoSpin Filters XL |
| Fragment size | 50 bp–approx. 50 kbp | 50 bp–approx. 50 kbp | 50 bp–approx. 50 kbp |
| Typical yield | 1–30 µg | 10–100 µg | 50–300 µg |
| A260/A280 | 1.8–1.9 | 1.8–1.9 | 1.8–1.9 |
| Elution volume | 50–100 µl | 200–400 µl | 1,000–2,000 µl |
| Preparation time | 30 min/prep | 90 min/prep | 90 min/prep |
| Binding capacity | 50 µg | 200 µg | 500 µg |
Product Overview
NucleoSpin Plant II
- Genomic DNA from plant and fungi with high yield and purity
- Two lysis buffers—optimal processing of any plant material
- NucleoSpin filters included for clarification of lysate
- Optimized buffer compositions and silica membrane for improved DNA binding
- RNase A included
- Process up to 100 mg (wet weight) or 20 mg (dry weight) of starting material
- Typical yields range from 1 to 30 μg of DNA (depending on the individual sample)
Second-generation kits for isolation of genomic DNA from plant material, fungi, and other biological samples
As plants are very heterogeneous and contain a lot of different metabolites like polyphenols, polysaccharides, or acidic components, NucleoSpin Plant II offers two different lysis procedures, based on CTAB and SDS lysis methods (with Lysis Buffer PL1 and PL2, respectively), for optimal processing of various samples. The silica membrane in each NucleoSpin Plant II column is optimized to improve DNA binding. NucleoSpin filters are included for convenient clarification of lysates. The eluted DNA is ready to use for subsequent reactions like PCR, restriction analysis, etc.
At a glance
Competitor comparison
Isolation of genomic DNA from different plant samples using NucleoSpin Plant II Maxi in comparison to Competitor Q. 1,500 mg of plant material was processed using Lysis Buffer PL1 and PL2 (NucleoSpin Plant II Maxi) or competitor product Q. 10 μl of each DNA eluate was analyzed on a 1% TAE-agarose gel.
Comparison to competitor's kits. 100 mg of fresh fir needles (Abies alba) were processed in triplicate using the NucleoSpin Plant II kit (Lysis Buffer PL1 and PL2) and competitors' products (Q, P, and I). 10 μl of each DNA eluate was analyzed on a 1% TAE-agarose gel.
Sample quality data
qPCR of DNA purified by NucleoSpin Plant II Maxi. Primers against conserved 18S rRNA show reliable quality of the DNA isolated from willow, rose, fir needle, and sugar beet.
Yield and purity data
Tabular overview of yield and purity for various plant samples isolated using NucleoSpin Plant II Maxi or Competitor Q.
| DNA yield (µg) | A260/A280 | A260/A280 | ||
| Willow | MN Q |
46 11 |
1.76 1.32 |
1.91 0.46 |
| Rose | MN Q |
24 7 |
1.73 1.21 |
1.62 0.43 |
| Fir needle | MN Q |
44 20 |
1.76 1.47 |
1.95 0.60 |
| Wheat | MN Q |
232 142 |
1.84 1.77 |
2.09 1.53 |
| Sugar beet | MN Q |
32 23 |
1.82 1.61 |
1.65 0.69 |
Plant varieties
PCR analysis of isolated genomic DNA from different plant samples using NucleoSpin Plant II. 100 mg of plant material was lysed with Buffer PL1 or PL2. PCR analysis was performed with 1–2 μl of each DNA eluate using primers against chloroplast tRNA and loaded on a 1% TAE-agarose gel. Lane M: marker, lane 1–18: analyzed samples (willow, rose, fir needles, wheat, sugar beet, spruce needles, cherry tree, grass, elder bush, geranium, yew, stinging nettle, dragon tree, yellow soybean, corn salad, garden cress, maize, and gladiolus).
Ordering Information
Universal reagent for RNA isolation
NucleoZOL is a highly efficient RNA isolation reagent for extracting high-quality miRNA and total RNA from a variety of starting materials, including cells, tissues, and liquids of human or animal origin, as well as plants, yeast, bacteria, viruses, and other sources.
See the data RNA purification kitsTakara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
