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Home › Learning centers › Nucleic acid purification › Genomic DNA purification › Plant › NucleoSpin Plant II

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Product Overview

NucleoSpin Plant II

  • Genomic DNA from plant and fungi with high yield and purity
  • Two lysis buffers—optimal processing of any plant material
  • NucleoSpin filters included for clarification of lysate
  • Optimized buffer compositions and silica membrane for improved DNA binding
  • RNase A included
  • Process up to 100 mg (wet weight) or 20 mg (dry weight) of starting material
  • Typical yields range from 1 to 30 μg of DNA (depending on the individual sample)

Second-generation kits for isolation of genomic DNA from plant material, fungi, and other biological samples

As plants are very heterogeneous and contain a lot of different metabolites like polyphenols, polysaccharides, or acidic components, NucleoSpin Plant II offers two different lysis procedures, based on CTAB and SDS lysis methods (with Lysis Buffer PL1 and PL2, respectively), for optimal processing of various samples. The silica membrane in each NucleoSpin Plant II column is optimized to improve DNA binding. NucleoSpin filters are included for convenient clarification of lysates. The eluted DNA is ready to use for subsequent reactions like PCR, restriction analysis, etc.

At a glance Competitor comparison Sample quality data Yield and purity data Plant varieties

At a glance  

Available formats

NucleoSpin Plant II

NucleoSpin mini spin column (green insert)

NucleoSpin Plant II Midi

NucleoSpin midi spin column

NucleoSpin Plant II Maxi

NucleoSpin maxi spin column

Technology Silica-membrane technology Silica-membrane technology Silica-membrane technology
Format Mini spin columns Midi spin columns Maxi spin columns
Sample material

<100 mg of plant material (wet wt)
<20 mg of plant material (dry wt)

<400 mg of plant material (wet wt)
<80 mg of plant material (dry wt)

<1,500 mg of plant material (wet wt)
<300 mg of plant material (dry wt)

Lysate clarification NucleoSpin Filters NucleoSpin Filters L NucleoSpin Filters XL
Fragment size 50 bp–approx. 50 kbp 50 bp–approx. 50 kbp 50 bp–approx. 50 kbp
Typical yield 1–30 µg 10–100 µg 50–300 µg
A260/A280 1.8–1.9 1.8–1.9 1.8–1.9
Elution volume 50–100 µl 200–400 µl 1,000–2,000 µl
Preparation time 30 min/prep 90 min/prep 90 min/prep
Binding capacity 50 µg 200 µg 500 µg

Competitor comparison  

NucleoSpin Plant II Maxi comparison of genomic DNA isolation from different samples

Isolation of genomic DNA from different plant samples using NucleoSpin Plant II Maxi in comparison to Competitor Q. 1,500 mg of plant material was processed using Lysis Buffer PL1 and PL2 (NucleoSpin Plant II Maxi) or competitor product Q. 10 μl of each DNA eluate was analyzed on a 1% TAE-agarose gel.

NucleoSpin comparison to competitor kits

Comparison to competitor's kits. 100 mg of fresh fir needles (Abies alba) were processed in triplicate using the NucleoSpin Plant II kit (Lysis Buffer PL1 and PL2) and competitors' products (Q, P, and I). 10 μl of each DNA eluate was analyzed on a 1% TAE-agarose gel.

Sample quality data  

qPCR of DNA purified by NucleoSpin Plant II Maxi

qPCR of DNA purified by NucleoSpin Plant II Maxi. Primers against conserved 18S rRNA show reliable quality of the DNA isolated from willow, rose, fir needle, and sugar beet.

Yield and purity data  

Tabular overview of yield and purity for various plant samples isolated using NucleoSpin Plant II Maxi or Competitor Q.

DNA yield (µg) A260/A280 A260/A280
Willow MN
Q
46
11
1.76
1.32
1.91
0.46
Rose MN
Q
24
7
1.73
1.21
1.62
0.43
Fir needle MN
Q
44
20
1.76
1.47
1.95
0.60
Wheat MN
Q
232
142
1.84
1.77
2.09
1.53
Sugar beet MN
Q
32
23
1.82
1.61
1.65
0.69

Plant varieties  

Purify DNA from a huge variety of plants

PCR analysis of isolated genomic DNA from different plant samples using NucleoSpin Plant II. 100 mg of plant material was lysed with Buffer PL1 or PL2. PCR analysis was performed with 1–2 μl of each DNA eluate using primers against chloroplast tRNA and loaded on a 1% TAE-agarose gel. Lane M: marker, lane 1–18: analyzed samples (willow, rose, fir needles, wheat, sugar beet, spruce needles, cherry tree, grass, elder bush, geranium, yew, stinging nettle, dragon tree, yellow soybean, corn salad, garden cress, maize, and gladiolus).

Ordering Information

Cat. # Product Size License Quantity Details
740770.250 NucleoSpin® Plant II 250 Preps USD $855.00

The NucleoSpin Plant II kit (250 preps) allows for the isolation of genomic DNA from up to 100 mg (wet weight) or 20 mg (dry weight) of plant tissue. The kit includes NucleoSpin Plant II Columns, NucleoSpin Filters, Collection Tubes, buffers, and RNase A.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue
NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue. The two different lysis buffers included in the kit allow you to purify a wide range of starting material.

Back

740770.250: NucleoSpin Plant II

740770.250: NucleoSpin Plant II
740770.250S NucleoSpin® Plant II Columns 250 Columns USD $423.00

Columns for NucleoSpin Plant II kits. NucleoSpin Plant II is ideal for rapid purification of plant genomic DNA using a tabletop centrifuge.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue
NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue. The two different lysis buffers included in the kit allow you to purify a wide range of starting material.

Back

740770.250S: NucleoSpin Plant II Columns

740770.250S: NucleoSpin Plant II Columns
740770.50 NucleoSpin® Plant II 50 Preps USD $197.00

The NucleoSpin Plant II kit (50 preps) allows for the isolation of genomic DNA from up to 100 mg (wet weight) or 20 mg (dry weight) of plant tissue. The kit includes NucleoSpin Plant II Columns, NucleoSpin Filters, Collection Tubes, buffers, and RNase A.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue
NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue. The two different lysis buffers included in the kit allow you to purify a wide range of starting material.

Back

740770.50: NucleoSpin Plant II

740770.50: NucleoSpin Plant II
740771.20 NucleoSpin® Plant II Midi 20 Preps USD $154.00

The NucleoSpin Plant II Midi kit (20 preps) allows for the isolation of genomic DNA from up to 250 mg (wet weight) of plant tissue. The kit includes NucleoSpin Plant II Midi Columns, NucleoSpin Filters L, Collection Tubes, buffers, and RNase A.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue
NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue. The two different lysis buffers included in the kit allow you to purify a wide range of starting material.

Back

740771.20: NucleoSpin Plant II Midi

740771.20: NucleoSpin Plant II Midi
740772.10 NucleoSpin® Plant II Maxi 10 Preps USD $130.00

The NucleoSpin Plant II Maxi kit (10 preps) allows for the isolation of genomic DNA from up to 1,000 mg (wet weight) of plant tissue. The kit includes NucleoSpin Plant II Maxi Columns, NucleoSpin Filters XL, Collection Tubes, buffers, and RNase A.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue
NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue. The two different lysis buffers included in the kit allow you to purify a wide range of starting material.

Back

740772.10: NucleoSpin Plant II Maxi

740772.10: NucleoSpin Plant II Maxi
740817.50 MN Bead Tubes Type G 50 Preps USD $190.00

MN Bead Tubes are 2-ml screw cap plastic tubes containing different types of beads depending on the application. They are intended for the disruption of biological samples and subsequent nucleic acid purification. MN Bead Tubes Type G consists of 5-mm steel beads for use with hard-to-lyse plant and fungal material using the NucleoSpin RNA Plant and Fungi kits. They should be used in combination with a swing mill (e.g., Mixer Mill MM200, MM300, MM400 from Retsch GmbH)

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

740817.50: NucleoSpin Bead Tubes Type G

740817.50: NucleoSpin Bead Tubes Type G
740770.10 NucleoSpin® Plant II 10 Preps USD $43.00

The NucleoSpin Plant II kit (10 preps) allows for the isolation of genomic DNA from up to 100 mg (wet weight) or 20 mg (dry weight) of plant tissue. The kit includes NucleoSpin Plant II Columns, NucleoSpin Filters, Collection Tubes, buffers, and RNase A.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

740770.10: NucleoSpin Plant II

740770.10: NucleoSpin Plant II


NucleoZOL RNA isolation reagent

Universal reagent for RNA isolation

NucleoZOL is a highly efficient RNA isolation reagent for extracting high-quality miRNA and total RNA from a variety of starting materials, including cells, tissues, and liquids of human or animal origin, as well as plants, yeast, bacteria, viruses, and other sources.

See the data RNA purification kits

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  • Try BcaBEST DNA Polymerase ver.2.0
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