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Genomic DNA purification

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NucleoMag DNA Bacteria NucleoMag DNA Bacteria product information
Magnetic separators Magnetic separators
Homogenization bead selection guide
Product overview

NucleoMag DNA Bacteria

NucleoMag DNA Bacteria employs a scalable, magnetic-bead-based technology to enable high-throughput isolation of genomic DNA from a wide variety of microbial cultures, including Gram-positive and Gram-negative bacteria, as well as yeast and other fungi. The kit can be used to process microbial culture samples used in industrial applications such as food research and chemical production, as well as on organisms relevant to clinical and ecological studies. DNA isolation can be performed manually or automated on standard liquid handling instruments and magnetic separators.

The NucleoMag DNA Bacteria kit offers a powerful but environmentally friendly method for isolating microbial genomic DNA, which avoids the use of chaotropic salts (Figure 1). It can be combined with bead-based homogenization methods, such as MN bead tubes or MN 96 bead plates, to achieve more efficient sample disruption. When compared with competitor kits, NucleoMag DNA Bacteria was shown to extract microbial DNA more effectively from a variety of sample types that included Gram-positive and Gram-negative bacteria and yeast—resulting in consistently lower Ct values when the isolated DNA was analyzed using qPCR (Figure 2). The NucleoMag DNA Bacteria kit was also shown to provide comparably efficient DNA extraction using either bead-plate or single-tube homogenization (Figure 3), demonstrating its versatility. DNA isolated using both homogenization methods was shown to be of reliable integrity (Figure 4), making it suitable for a variety of downstream applications, including PCR, qPCR, and NGS.

The NucleoMag DNA Bacteria kit offers the following benefits:

  • Versatile high-throughput DNA extraction method suitable for a diverse array of microbes, including Gram-negative and Gram-positive bacteria and fungi
  • Scalable magnetic-bead-based purification technology can be easily automated on common liquid handling platforms
  • Environmentally friendly buffer chemistry avoids the use of chaotropic salts and hazardous materials
  • Liquid Proteinase K and Liquid RNase A are provided for easy handling
  • MN bead tubes or MN 96 bead plates may be combined with the kit for more efficient sample disruption
  • Support protocols are available for DNA extraction from hard-shelled organisms such as insects and crustaceans, as well as lipid-rich and fungal samples

At a glance  

Technology Magnetic-bead technology
Processing Manual or automated
Sample material Cell culture pellets of Gram-positive or Gram-negative bacteria, yeasts, or other fungi
Sample amount Up to approx. 40 mg wet weight
Sample types tested

Bacteria: E. coli, B. subtilis, C. glutamicum, V. fischeri
Yeast: Yeast (S. cerevisiae), bread mold (R. stolonifer)*, melon mold*
Insects and crustacea:* Mealworm (T. molitor), fruit fly (D. melanogaster), freshwater shrimp (D. pulex), honey bees, cockroaches, isopods, juvenile house crickets (A. domesticus), firebrat (T. domestica), shrimps, field cricket (G. assimilis), mosquitos, mosquito larvae (Anopheles spec.)
Fatty tissue:* Mouse brain, mouse testicles (M. musculus), atlantic salmon (S. salar), river trout (S. trutta fario)
Elution volume 50–200 µl
Preparation time 30 min on KingFisher Flex (excl. sample lysis)
Theoretical binding capacity 0.4 µg/µl beads

*Support protocol available. Please contact technical support.

Applications

  • Isolation of DNA from microbial cell cultures
  • Typical downstream applications: PCR, qPCR, NGS

Procedure  

NucleoMag DNA Bacteria procedure

Figure 1. NucleoMag DNA Bacteria procedure. For optimal DNA yields, complete disruption of sample material is necessary and can be performed using MN bead tubes and/or MN 96 bead plates. After sample disruption, Binding Buffer IMB and NucleoMag B-Beads are added to the transferred lysate. Following magnetic separation, NucleoMag B-Beads are washed to remove contaminants and salts using Wash Buffer IMW and 80% ethanol, respectively. After air-drying the NucleoMag B-Beads for 10 min at room temperature, the DNA is finally eluted with Elution Buffer IME. The NucleoMag DNA Bacteria kit can be used either for manual processing or it can be automated on standard liquid handling instruments and magnetic separators.

Application data  

More efficient extraction of microbial DNA than competitor kits

Figure 2. More efficient extraction of microbial DNA than competitor kits. DNA was isolated from Gram-positive and Gram-negative bacteria as well as yeast using the NucleoMag DNA Bacteria kit (MN, blue bars) in comparison with Competitor Q (gray bars, Panel A) or Competitor T (gray bars, Panel B) according to the manufacturers' recommendations. The isolated DNA was then analyzed by qPCR analysis of 16s and 18s rRNA sequences using the Maxima SYBR Green kit by Thermo Fisher Scientific on an Applied Biosystems 7500 Real-Time PCR System. Resulting Ct values were consistently lower for DNA isolated using NucleoMag DNA Bacteria than Ct values obtained with DNA isolated using the competitor kits, suggesting that NucleoMag DNA Bacteria provided more efficient extraction of genomic DNA from the various samples.

Efficient DNA extraction using either bead-plate or single-tube homogenization

Figure 3. Efficient DNA extraction using either bead-plate or single-tube homogenization. DNA was isolated from various Gram-positive and Gram-negative bacteria using the NucleoMag DNA Bacteria kit in combination with different bead formats for sample homogenization and resulting DNA yields were measured by UV spectrometry. The samples were homogenized by using either racks of prefilled tube strips (MN 96 bead plates) or single bead tubes (MN bead tubes). The resulting yields for samples homogenized with MN 96 bead plates (dark blue bars) were comparable to the yields obtained via homogenization in the MN bead tubes (light blue bars).

Reliable DNA integrity with bead-plate or bead-tube homogenization

Figure 4. Reliable DNA integrity with bead-plate or bead-tube homogenization. DNA was isolated from Gram-positive and Gram-negative bacteria using the NucleoMag DNA Bacteria kit, and samples were homogenized in parallel by using either a rack of prefilled tube strips (BP = MN 96 bead plate) or single bead tubes (BT = MN bead tube). Both homogenization approaches enabled reliable DNA extraction when used in tandem with the NucleoMag DNA Bacteria kit.

Related Products

Cat. # Product Size License Quantity Details
744310.1 NucleoMag® DNA Bacteria 1 x 96 Preps USD $281.00

The NucleoMag DNA Bacteria kit employs scalable magnetic bead technology to enable efficient, high-throughput DNA purification from microbial cultures consisting of Gram-positive or Gram-negative bacteria or fungi such as yeast. The kit workflow can be modified with the addition of a sample homogenization step employing MN Bead Tubes or MN 96 Bead Plates to provide improved sample lysis, and the protocol can be easily automated on common liquid handling platforms. NucleoMag DNA Bacteria utilizes a powerful yet environmentally friendly buffer chemistry that is free of chaotropic salts or hazardous components. Typical downstream applications for DNA purified with the kit include PCR, qPCR, and NGS.

Catalog # 744310.1 includes sufficient quantities of reagents and materials for processing 96 samples at the volumes indicated in the kit protocol.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
744310.4 NucleoMag® DNA Bacteria 4 x 96 Preps USD $1040.00

The NucleoMag DNA Bacteria kit employs scalable magnetic bead technology to enable efficient, high-throughput DNA purification from microbial cultures consisting of Gram-positive or Gram-negative bacteria or fungi such as yeast. The kit workflow can be modified with the addition of a sample homogenization step employing MN Bead Tubes or MN 96 Bead Plates to provide improved sample lysis, and the protocol can be easily automated on common liquid handling platforms. NucleoMag DNA Bacteria utilizes a powerful yet environmentally friendly buffer chemistry that is free of chaotropic salts or hazardous components. Typical downstream applications for DNA purified with the kit include PCR, qPCR, and NGS.

Catalog # 744310.4 includes sufficient quantities of reagents and materials for processing 384 samples at the volumes indicated in the kit protocol.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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