|Technology||Magnetic bead technology|
|Format||Highly reactive superparamagnetic beads|
|Processing||Manual or automated|
|Starting material||Swabs (cotton or synthetic)|
|Sample amount||300 µl reconstituted swab lysate|
|Fragment size||>300 bp–approximately 50 kb (depending on sample processing)|
|Typical yield||1–3 µg DNA (depending on sample amount and quality)|
|Typical concentration||10–30 ng/µl|
|Elution volume||50–100 µl|
|Preparation time||120 min/96 preps (manual preparation)
30 min/96 preps (automated on KingFisher Flex; excluding lysis)
|Theoretical binding capacity||0.4 μg/μl beads|
NucleoMag DNA Swab
The NucleoMag DNA Swab kit is designed for isolation of genomic DNA (human and/or microbial) from a wide variety of swab specimens, including dental, nasopharyngeal, and vaginal swabs as well as other samples, such as cigarette filters and dried blood spots. The kit is compatible with commonly used cotton swabs as well as synthetic swabs (e.g., flocked swabs) and may be used to prepare samples for high-throughput genetic testing. The NucleoMag DNA Swab kit can be used either manually or on automated liquid-handling instruments (such as Hamilton, Tecan, and Eppendorf platforms) and magnetic separators (such as the KingFisher Duo and Flex).
The kit procedure is based on reversible adsorption of nucleic acids to highly reactive superparamagnetic beads. Highly pure DNA is eluted with a low-salt elution buffer and can be used directly for downstream applications such as qPCR, NGS, or STR analysis, as well as any kind of enzymatic reaction. Application data for high-throughput genomic DNA isolation from diverse swab samples demonstrates high yields and sensitive qPCR detection (Figures 1 and 2 below, respectively).
The NucleoMag DNA Swab kit offers the following benefits:
- High-throughput DNA isolation for genetic testing
- Developed for cotton as well as synthetic swabs
- Small elution volumes—as low as 50 µl
- Suitable for manual or automated processing
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