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Magnetic bead-based isolation of genomic DNA from swabs—NucleoMag DNA Swab
The NucleoMag DNA Swab kit enables manual or automated purification of genomic DNA from swab specimens using proven NucleoMag magnetic bead technology. NucleoMag DNA Swab can isolate human and/or microbial gDNA from cotton swabs and synthetic swabs (e.g., flocked swabs). It is designed for isolation of gDNA from common epithelial surfaces, including dental, nasopharyngeal, and vaginal swabs as well as other samples including cigarette filters and dried blood spots. After sample lysis with Proteinase K, gDNA is purified from 300 µl of sample. The entire NucleoMag DNA Swab protocol can be automated on common liquid handling platforms.
The NucleoMag DNA Swab kit enables manual or automated purification of genomic DNA from swab specimens using proven NucleoMag magnetic bead technology. NucleoMag DNA Swab can isolate human and/or microbial gDNA from cotton swabs and synthetic swabs (e.g., flocked swabs). It is designed for isolation of gDNA from common epithelial surfaces, including dental, nasopharyngeal, and vaginal swabs as well as other samples, such as cigarette filters and dried blood spots. After sample lysis with Proteinase K, gDNA is purified from 300 µl of sample. The entire NucleoMag DNA Swab protocol can be automated on common liquid handling platforms.
To simplify the separation of swab material from lysate, the kit can be combined with NucleoSpin Forensic Filters or the NucleoSpin Trace Filter Plate (please note that the NucleoSpin Trace Filter Plate requires additional material for operating under vacuum or centrifugation). The NucleoMag DNA Swab kit can be used either manually or on automated liquid handling instruments (such as Hamilton, Tecan and Eppendorf platforms) and magnetic separators (such as the KingFisher Duo and Flex).
The NucleoMag product family is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure DNA can be eluted from the beads using elution buffer. NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.
Over thirty human pathogens have been isolated from swabs with the NucleoMag DNA Swab kit and successfully detected by qPCR, including:
|Species||Pathogen type||Ct value|
|Acetinobacter baumanii||Gram negative bacteria||23.05|
|Atopobium vaginae||Gram positive bacteria||19.92|
|Bacteriodes fragilis||Gram negative bacteria||21.66|
|Chlamydia trachomatis||Gram negative bacteria||24.31, 23.90|
|Citrobacter freundii||Gram negative bacteria||22.78|
|Enterococcus faecalis||Gram positive bacteria||22.27|
|Escherichia coli||Gram negative bacteria||26.05|
|Gardnerella vaginalis||Gram negative and gram positive bacteria||16.79|
|Herpes simplex type 1||dsDNA virus||27.92, 27.44|
|Herpes simplex type 2||dsDNA virus||21.60, 21.49|
|Klebsiella oxytoca||Gram negative bacteria||26.86|
|Lactobacillus jensenii||Gram positive bacteria||15.30|
|Mobiluncus mulieris||Gram negative and gram positive bacteria||27.75|
|Morganella morganii||Gram negative bacteria||23.14|
|Mycoplasma genitalium||Mycobacteria||15.33, 15.17|
|Neisseria gonorrhoeae||Gram negative bacteria||14.93, 14.91|
|Prevotella bivia||Gram negative bacteria||24.48|
|Proteus mirabilis||Gram negative bacteria||22.81|
|Proteus vulgaris||Gram negative bacteria||23.10|
|Providencia stuartii||Gram negative bacteria||22.90|
|Pseudomonas aeruginosa||Gram negative bacteria||18.56|
|Serratia marcescens||Gram negative bacteria||24.13|
|Staphylococcus aureus||Gram positive bacteria||22.50|
|Staphylococcus saprophyticus||Gram positive bacteria||24.63|
|Streptococcus agalactiae||Gram positive bacteria||23.06|
|Trichomonas vaginalis||Protozoan||24.84, 25.20|
Note: An additional pre-digestion step with Lysis Buffer FLB and Liquid Proteinase K significantly improves the lysis of some pathogens—particularly yeast—and may be required for positive results.
- High-throughput DNA isolation for genetic testing
- Developed for cotton as well as synthetic swabs
- Small elution volumes—as low as 50 µl
- Suitable for manual or automated processing
|Technology||Magnetic bead technology|
|Format||Highly reactive superparamagnetic beads|
|Processing||Manual or automated|
|Starting material||Swabs (cotton or synthetic)|
|Sample amount||300 µl reconstituted swab lysate|
|Fragment size||>300 bp–approximately 50 kb (depending on sample processing)|
|Typical yield||1–3 µg DNA (depending on sample amount and quality)|
|Typical concentration||10–30 ng/µl|
|Elution volume||50–100 µl|
|Preparation time||120 min/96 preps (manual preparation)
30 min/96 preps (automated on KingFisher Flex; excluding lysis)
|Theoretical binding capacity||0.4 μg/μl beads|
- High-throughput genetic testing
- Manual or automated isolation of DNA from cotton or synthetic swabs (e.g., COPAN FLOQSwabs and Puritan HydraFlock swabs)
- Typical downstream applications: qPCR, NGS, STR analysis, enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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