Isolation of genomic DNA from forensic samples—NucleoSpin DNA Trace
NucleoSpin DNA Trace is designed for the preparation of highly pure genomic DNA from small amounts of any tissue, cell, or forensic sample. The NucleoSpin Funnel Columns (NucleoSpin DNA Trace F Columns) included in the kit are ideally suited for collecting trace amounts of nucleic acids from large volumes of input material. The optimized shape of the NucleoSpin Funnel Column allows the combination of a large volume capacity and a small binding membrane diameter.
NucleoSpin DNA Trace is designed for the preparation of highly pure genomic DNA from small amounts of any tissue, cell, or forensic sample. The NucleoSpin Funnel Columns (NucleoSpin DNA Trace F Columns) included in the kit are ideally suited for collecting trace amounts of nucleic acids from large volumes of input material. The optimized shape of the NucleoSpin Funnel Column allows the combination of a large volume capacity and a small binding membrane diameter. DNA isolated by NucleoSpin DNA Trace is ready to use for common downstream applications such as PCR, real-time PCR, or other enzymatic reactions.
The small diameter of the silica membrane allows elution volumes as low as 50 to 100 µl (of low-salt buffer or water), leading to highly enriched samples. 8 to 12 NucleoSpin Funnel Columns can be processed in parallel, depending on the centrifuge rotor used. The risk of cross-contamination is eliminated by placing the funnel columns into 50-ml polyethylene tubes which can be closed with screw caps, resulting in self-contained systems. For elution, 0.5-ml elution tubes are fitted directly to the outlet of the funnel columns (collection tubes and elution tubes are provided with the kit).
Overview
- Sample extraction with up to 8 ml of lysis buffer
- Small elution volume (100 µl) for highly concentrated DNA
- Closed system, no cross-contamination
- NucleoSpin Funnel Columns—patented technology
- Support protocol for isolation of genomic DNA from human bones
More Information
| Technology | Silica membrane |
| Format | Funnel columns |
| Starting material | Forensic samples (e.g., buccal swabs, blood spots) |
| Typical yield | >10 ng |
| Typical recovery | >70% |
| A260/280 | 1.7–1.9 |
| Elution volume | 100 µl |
| Preparation time | 60 min/prep |
| Binding capacity | 20 µg |
Applications
- DNA isolation from forensic samples (e.g., blood spots, swabs, etc.)
- For trace analysis and forensic investigations
- Obtained DNA is suitable for:
- PCR
- Real-time PCR
- Enzymatic reactions
Additional product information
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
Learn more All genomic DNA kitsTakara Bio USA, Inc.
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