Single-prep isolation of genomic DNA from blood with NucleoSpin Blood kits
NucleoSpin Blood, Blood L, Blood XL, and Blood QuickPure kits are designed for isolating genomic DNA from whole blood, serum, plasma, buffy coats, and cultured cells in a convenient silica membrane-based spin column format. Maximum starting volumes range from 200 µl of blood for the NucleoSpin Blood and Blood QuickPure mini kits to 2 ml for the Blood L midi kit and 10 ml for the Blood XL maxi kit.
NucleoSpin Blood, Blood L, Blood XL, and Blood QuickPure kits are designed for isolating genomic DNA from whole blood, serum, plasma, buffy coats, and cultured cells in a convenient silica membrane-based spin column format. Maximum starting volumes range from 200 µl of blood for the NucleoSpin Blood and Blood QuickPure mini kits to 2 ml for the Blood L midi kit and 10 ml for the Blood XL maxi kit. The NucleoSpin Blood QuickPure kits are ultra-fast mini kits that include spin columns with a specially treated silica membrane which speeds up the procedure by allowing a combined washing/drying step. This reduces the number of washing and drying steps from three to only one, resulting in a hands-on time of less than 10 minutes. DNA purified with any of these kits can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.
NucleoSpin Blood mini kits only (not the Blood QuickPure & Blood L/XL kits) may also be used for purifying viral DNA for a wide variety of viruses from blood and biological fluids (including HBV, CMV, HPV, TTV, and EBV). The Proteinase K step included in the NucleoSpin Blood method removes viral proteins, allowing the viral DNA in the lysate to bind to the silica membrane. Since viral DNA copurifies with cellular DNA, we recommend using cell-free samples (serum or plasma) to prepare pure viral DNA. The purified DNA is suitable for typical downstream applications such as real-time PCR and enzymatic reactions.
Overview
NucleoSpin Blood
- DNA is ready to use in less than 30 minutes
- Complete removal of PCR inhibitors
- Suitable for whole blood treated with citrate, EDTA, heparin, CPDA
- Also suitable for viral DNA isolation
- Consistently high DNA yields
- Cat. # 740951.240C is designed for use with the QIAcube only
NucleoSpin Blood QuickPure
- Ultra-fast procedure (10 minutes hands-on time)
- Washing and drying step are combined into one step
- Consistently high DNA yields
- Elute in only 30–50 µl
- Highly concentrated DNA, ready to use for sensitive downstream applications
NucleoSpin Blood L/XL
- Medium- and large-scale DNA purification from blood
- Complete removal of PCR inhibitors
- Suitable for whole blood treated with citrate, EDTA, heparin, CPDA
- Consistently high DNA yields
NucleoSpin Blood L Vacuum
- Efficient DNA purification from 2-ml blood samples using vacuum filtration
- Complete removal of PCR inhibitors
- Suitable for whole blood treated with EDTA or citrate
- Consistently high DNA yields
- Parallel purification of 24 samples in 75 minutes using the NucleoVac 96 Vacuum Manifold (Cat. # 740681) and the Starter Set Midi (Cat. # 740744)
More Information
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NucleoSpin Blood/Blood QuickPure
| NucleoSpin Blood | NucleoSpin Blood QuickPure | |
| Technology |
Silica membrane |
|
| Format |
Mini spin columns |
|
| Starting material |
1–200 µl whole blood, serum, plasma, body fluids |
1–200 µl whole blood (human or animal, fresh or frozen), body fluids <5 x 106 cultured cells (lymphocytes) |
| Fragment size |
200 bp–>30 kb |
|
| Typical yield |
4–6 µg |
|
| A260/280 |
1.6–1.9 |
|
| Typical concentration |
40–100 ng/µl |
80–120 ng/µl |
| Elution volume |
60–200 µl |
30–50 µl |
| Preparation time |
~30 min/prep |
~25 min/prep (<10 minutes hands-on-time) |
| Binding capacity |
60 µg |
50 µg |
-
NucleoSpin Blood L/XL
|
NucleoSpin Blood L |
NucleoSpin Blood XL |
|
| Technology |
Silica membrane |
|
| Format |
Midi spin columns |
Maxi spin columns |
| Starting material |
0.2–2 ml whole blood |
2–10 ml whole blood |
| Fragment size |
200 bp–>30 kb |
|
| Typical yield |
40–60 µg |
200–300 µg |
| A260/280 |
1.6–1.9 |
|
| Typical concentration |
200–500 ng/µl |
|
| Elution volume |
120–200 µl |
600–1,000 µl |
| Preparation time |
~60 min/prep |
~60 min/prep |
| Binding capacity |
250 µg |
700 µg |
-
NucleoSpin Blood L Vacuum
| Technology | Silica membrane |
| Format | Midi spin columns |
| Starting material | 1–2 ml whole blood |
| Fragment size | 200 bp–approx. 50 kb |
| Typical yield | 50–80 µg (2 ml blood) |
| A260/280 | 1.6–1.9 |
| Typical concentration | 100–130 ng/µl |
| Elution volume | 2 x 300 µl |
| Preparation time | 75 min/24 preps |
| Binding capacity | 250 µg |
Applications
- NucleoSpin Blood
- Genomic, bacterial, viral DNA isolation
- DNA from whole blood (human or animal, fresh or frozen)
- DNA from whole blood treated with citrate, EDTA, heparin, CPDA
- DNA from serum, plasma, buffy coats, platelets, body fluids (e.g., amniotic fluid)
- DNA from cultured cells
- DNA suitable for:
- PCR
- Southern blotting
- Enzymatic reactions
- NucleoSpin Blood QuickPure & Blood L/XL
- DNA from whole blood (human or animal, fresh or frozen)
- DNA from whole blood treated with citrate, EDTA, heparin, CPDA
- DNA from serum, plasma, buffy coats, platelets, body fluids (e.g., amniotic fluid)
- DNA from cultured cells
- DNA suitable for:
- PCR
- Southern blotting
- Enzymatic reactions
- NucleoSpin Blood L Vacuum
- DNA from whole blood (human or animal, fresh or frozen)
- DNA from whole blood treated with citrate, EDTA
- DNA suitable for:
- PCR
- Southern blotting
- Enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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