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  • NucleoBond CB
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Use the dropdown list to select the nucleic acid purification kit for your application Product finder
Isolate circulating DNA from plasma NucleoSnap DNA Plasma

Single-prep isolation of genomic DNA from blood with NucleoSpin Blood kits

Isolation of genomic DNA from blood.

NucleoSpin Blood, Blood L, Blood XL, and Blood QuickPure kits are designed for isolating genomic DNA from whole blood, serum, plasma, buffy coats, and cultured cells in a convenient silica membrane-based spin column format. Maximum starting volumes range from 200 µl of blood for the NucleoSpin Blood and Blood QuickPure mini kits to 2 ml for the Blood L midi kit and 10 ml for the Blood XL maxi kit. 

NucleoSpin Blood, Blood L, Blood XL, and Blood QuickPure kits are designed for isolating genomic DNA from whole blood, serum, plasma, buffy coats, and cultured cells in a convenient silica membrane-based spin column format. Maximum starting volumes range from 200 µl of blood for the NucleoSpin Blood and Blood QuickPure mini kits to 2 ml for the Blood L midi kit and 10 ml for the Blood XL maxi kit. The NucleoSpin Blood QuickPure kits are ultra-fast mini kits that include spin columns with a specially treated silica membrane which speeds up the procedure by allowing a combined washing/drying step. This reduces the number of washing and drying steps from three to only one, resulting in a hands-on time of less than 10 minutes. DNA purified with any of these kits can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.

NucleoSpin Blood mini kits only (not the Blood QuickPure & Blood L/XL kits) may also be used for purifying viral DNA for a wide variety of viruses from blood and biological fluids (including HBV, CMV, HPV, TTV, and EBV). The Proteinase K step included in the NucleoSpin Blood method removes viral proteins, allowing the viral DNA in the lysate to bind to the silica membrane. Since viral DNA copurifies with cellular DNA, we recommend using cell-free samples (serum or plasma) to prepare pure viral DNA. The purified DNA is suitable for typical downstream applications such as real-time PCR and enzymatic reactions.

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Cat. # Product Size Price License Quantity Details
740951.50 NucleoSpin® Blood 50 Preps USD $127.00

NucleoSpin Blood (50) 50 preps for the isolation of genomic DNA from blood - NucleoSpin Blood Columns, Collection Tubes, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure. Lane M: DNA size marker. Lane 1: Purification with NucleoSpin Blood kit. Lane 2: Purification with NucleoSpin Blood QuickPure kit.

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740951.50: NucleoSpin Blood

740951.50: NucleoSpin Blood
740951.250 NucleoSpin® Blood 250 Preps USD $540.00

NucleoSpin Blood (250) 250 preps for the isolation of genomic DNA from blood - NucleoSpin Blood Columns, Collection Tubes, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure. Lane M: DNA size marker. Lane 1: Purification with NucleoSpin Blood kit. Lane 2: Purification with NucleoSpin Blood QuickPure kit.

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740951.250: NucleoSpin Blood

740951.250: NucleoSpin Blood
740951.240C NucleoSpin® Blood 240 Preps USD $540.00

NucleoSpin Blood (240) 240 preps for the isolation of genomic DNA from blood Buffers, Proteinase K, NucleoSpin Blood Columns

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740951.240C: NucleoSpin Blood

740951.240C: NucleoSpin Blood
740954.20 NucleoSpin® Blood L 20 Preps USD $159.00

The NucleoSpin Blood L kit enables rapid purification of DNA from up to 2 ml of blood—including serum, plasma, buffy coat, platelets, etc.—using a simple procedure that can be completed in an hour. The kit protocol is compatible with citrate-, EDTA-, and CPDA-treated samples; enables efficient removal of PCR inhibitors; and typically yields 40–60 µg of DNA per prep. The kit consists of NucleoSpin Blood L Columns, Collection Tubes, buffers, and Proteinase K.

Cat. # 740954.20 includes sufficient quantities of reagents and materials for processing 20 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure. Lane M: DNA size marker. Lane 1: Purification with NucleoSpin Blood kit. Lane 2: Purification with NucleoSpin Blood QuickPure kit.

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740954.20: NucleoSpin Blood L

740954.20: NucleoSpin Blood L
740954.24 NucleoSpin® Blood L Vacuum 24 Preps USD $197.00

NucleoSpin Blood L Vacuum (24) 24 preps for the isolation of genomic DNA from blood under vacuum- NucleoSpin Blood L Columns, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740954.24: NucleoSpin Blood L Vacuum

740954.24: NucleoSpin Blood L Vacuum
740569.50 NucleoSpin® Blood QuickPure 50 Preps USD $128.00

NucleoSpin Blood QuickPure (50) 50 preps for the isolation of genomic DNA from blood - NucleoSpin Blood QuickPure Columns, Collection Tubes, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure. Lane M: DNA size marker. Lane 1: Purification with NucleoSpin Blood kit. Lane 2: Purification with NucleoSpin Blood QuickPure kit.

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740569.50: NucleoSpin Blood QuickPure

740569.50: NucleoSpin Blood QuickPure
740569.250 NucleoSpin® Blood QuickPure 250 Preps USD $555.00

NucleoSpin Blood QuickPure (250) 250 preps for the isolation of genomic DNA from blood - NucleoSpin Blood QuickPure Columns, Collection Tubes, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure. Lane M: DNA size marker. Lane 1: Purification with NucleoSpin Blood kit. Lane 2: Purification with NucleoSpin Blood QuickPure kit.

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740569.250: NucleoSpin Blood QuickPure

740569.250: NucleoSpin Blood QuickPure
740950.50 NucleoSpin® Blood XL 50 Preps USD $720.00

The NucleoSpin Blood XL kit enables rapid purification of DNA from up to 2 ml of blood—including serum, plasma, buffy coat, platelets, etc.—using a simple procedure that can be completed in an hour. The kit protocol is compatible with citrate-, EDTA-, and CPDA-treated samples; enables efficient removal of PCR inhibitors; and typically yields 200–300 µg of DNA per prep. The kit consists of NucleoSpin Blood XL Columns, Collection Tubes, buffers, and Proteinase K.

Cat. # 740950.50 includes sufficient quantities of reagents and materials for processing 50 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure. Lane M: DNA size marker. Lane 1: Purification with NucleoSpin Blood kit. Lane 2: Purification with NucleoSpin Blood QuickPure kit.

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740950.50: NucleoSpin Blood XL

740950.50: NucleoSpin Blood XL
740950.10 NucleoSpin® Blood XL 10 Preps USD $146.00

The NucleoSpin Blood XL kit enables rapid purification of DNA from up to 2 ml of blood—including serum, plasma, buffy coat, platelets, etc.—using a simple procedure that can be completed in an hour. The kit protocol is compatible with citrate-, EDTA-, and CPDA-treated samples; enables efficient removal of PCR inhibitors; and typically yields 200–300 µg of DNA per prep. The kit consists of NucleoSpin Blood XL Columns, Collection Tubes, buffers, and Proteinase K.

Cat. # 740950.10 includes sufficient quantities of reagents and materials for processing 10 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure

DNA purification from human blood (200 μl) with NucleoSpin Blood and NucleoSpin Blood QuickPure. Lane M: DNA size marker. Lane 1: Purification with NucleoSpin Blood kit. Lane 2: Purification with NucleoSpin Blood QuickPure kit.

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740950.10: NucleoSpin Blood XL

740950.10: NucleoSpin Blood XL

Overview

NucleoSpin Blood

  • DNA is ready to use in less than 30 minutes
  • Complete removal of PCR inhibitors
  • Suitable for whole blood treated with citrate, EDTA, heparin, CPDA
  • Also suitable for viral DNA isolation
  • Consistently high DNA yields
  • Cat. # 740951.240C is designed for use with the QIAcube only

NucleoSpin Blood QuickPure

  • Ultra-fast procedure (10 minutes hands-on time)
  • Washing and drying step are combined into one step
  • Consistently high DNA yields
  • Elute in only 30–50 µl
  • Highly concentrated DNA, ready to use for sensitive downstream applications

NucleoSpin Blood L/XL

  • Medium- and large-scale DNA purification from blood
  • Complete removal of PCR inhibitors
  • Suitable for whole blood treated with citrate, EDTA, heparin, CPDA
  • Consistently high DNA yields

NucleoSpin Blood L Vacuum

  • Efficient DNA purification from 2-ml blood samples using vacuum filtration
  • Complete removal of PCR inhibitors
  • Suitable for whole blood treated with EDTA or citrate
  • Consistently high DNA yields
  • Parallel purification of 24 samples in 75 minutes using the NucleoVac 96 Vacuum Manifold (Cat. # 740681) and the Starter Set Midi (Cat. # 740744)

More Information

  • NucleoSpin Blood/Blood QuickPure

NucleoSpin Blood NucleoSpin Blood QuickPure
Technology

Silica membrane

Format

Mini spin columns

Starting material

1–200 µl whole blood, serum, plasma, body fluids
<5 x 106 cultured cells

1–200 µl whole blood (human or animal, fresh or frozen), body fluids
<5 x 106 cultured cells (lymphocytes)
Fragment size

200 bp–>30 kb

Typical yield

4–6 µg

A260/280

1.6–1.9

Typical concentration

40–100 ng/µl

80–120 ng/µl

Elution volume

60–200 µl

30–50 µl

Preparation time

~30 min/prep

~25 min/prep (<10 minutes hands-on-time)

Binding capacity

60 µg

50 µg

  

  • NucleoSpin Blood L/XL

NucleoSpin Blood L

NucleoSpin Blood XL

Technology

Silica membrane

Format

Midi spin columns

Maxi spin columns

Starting material

0.2–2 ml whole blood
5 x 106 cultured cells

2–10 ml whole blood
1 x 108 cultured cells

Fragment size

200 bp–>30 kb

Typical yield

40–60 µg

200–300 µg

A260/280

1.6–1.9

Typical concentration

200–500 ng/µl

Elution volume

120–200 µl

600–1,000 µl

Preparation time

~60 min/prep

~60 min/prep

Binding capacity

250 µg

700 µg

   

  • NucleoSpin Blood L Vacuum

Technology Silica membrane
Format Midi spin columns
Starting material 1–2 ml whole blood 
Fragment size 200 bp–approx. 50 kb
Typical yield  50–80 µg (2 ml blood)
A260/280 1.6–1.9
Typical concentration 100–130 ng/µl
Elution volume 2 x 300 µl
Preparation time 75 min/24 preps
Binding capacity 250 µg

Applications

  • NucleoSpin Blood
    • Genomic, bacterial, viral DNA isolation
    • DNA from whole blood (human or animal, fresh or frozen)
    • DNA from whole blood treated with citrate, EDTA, heparin, CPDA
    • DNA from serum, plasma, buffy coats, platelets, body fluids (e.g., amniotic fluid)
    • DNA from cultured cells
    • DNA suitable for:
      • PCR
      • Southern blotting
      • Enzymatic reactions
  • NucleoSpin Blood QuickPure & Blood L/XL
    • DNA from whole blood (human or animal, fresh or frozen)
    • DNA from whole blood treated with citrate, EDTA, heparin, CPDA
    • DNA from serum, plasma, buffy coats, platelets, body fluids (e.g., amniotic fluid)
    • DNA from cultured cells
    • DNA suitable for:
      • PCR
      • Southern blotting
      • Enzymatic reactions
  • NucleoSpin Blood L Vacuum
    • DNA from whole blood (human or animal, fresh or frozen)
    • DNA from whole blood treated with citrate, EDTA
    • DNA suitable for:
      • PCR
      • Southern blotting
      • Enzymatic reactions

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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