Isolation of genomic DNA from lipid-rich tissue—NucleoSpin DNA Lipid Tissue
The NucleoSpin DNA Lipid Tissue kit is designed for rapid purification of highly pure genomic DNA from any type of tissue with a high lipid content such as brain, adipose, or fish tissue. The kit utilizes MN Bead Tubes Type D (steel beads; included in the kit) in combination with a common disruption device, such as an MN Bead Tube Holder or a swing mill, to lyse different cell types and tissues using a single procedure. MN bead tubes can also be ordered separately if needed.
Overview
- Special buffer composition for efficient removal of lipids
- Includes MN bead tubes for efficient lysis—compatible with the most common disruption devices
- Fast and convenient procedure without RNA contamination
More Information
| Technology | Silica-membrane technology combined with MN Bead Tubes Type D |
| Format | Mini spin columns |
| Starting material | <40 mg fresh or frozen, lipid-rich tissue (e.g., brain, adipose tissue, fatty fish tissue) |
| Fragment size | 200 bp–approximately 50 kb |
| Typical yield | Depends on sample type, quality, and water content |
| A260/A280 | 1.7–1.9 |
| Elution volume | 25–200 µl |
| Preparation time | 35 min/6 preps |
| Binding capacity | 60 µg |
Applications
- Genomic DNA from any type of tissue with a high lipid content
- Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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