Isolation of genomic DNA from food and Feed—NucleoSpin 8/96 Food

NucleoSpin 8/96 Food

The NucleoSpin 8/96 Food kits allow you to quickly isolate genomic DNA from many types of processed food or animal feed of plant or animal origin in a high-throughput format. Food samples are highly heterogeneous and contain many different compounds such as fats and polysaccharides. These characteristics make DNA extraction more difficult. The NucleoSpin 8/96 Food protocol guarantees good recovery rates for small genomic DNA fragments from food.

The NucleoSpin 8/96 Food kits allow you to quickly isolate genomic DNA from many types of processed food or animal feed of plant or animal origin in a high-throughput format. Food samples are highly heterogeneous and contain many different compounds such as fats and polysaccharides. These characteristics make DNA extraction more difficult. The NucleoSpin 8/96 Food protocol guarantees good recovery rates for small genomic DNA fragments from food. We have optimized the lysis buffer and purification process to extract DNA from a variety of food products. The purified DNA is ready to use for all types of subsequent detection methods, especially as a template for PCR. Isolation of genomic DNA from food products assists in detecting and identifying contamination, genetically modified organisms (GMOs), or individual plant or animal species.

The NucleoSpin Food kit allows you to process up to 200 mg of homogenized material using manual or automated methods, via vacuum or centrifugation. The silica-membrane spin technology has been optimized for high DNA recovery and low binding of impurities, so the kit generates pure, ready-to-use DNA from any type of food.

Overview

  • Flexible high-throughput format: 8-well strips or 96-well plates
  • Complete removal of PCR inhibitors
  • DNA is ready to use for real-time PCR
  • Processing is possible under vacuum or by centrifugation
  • Suitable for manual or automated processing

More Information

NucleoSpin 8 Food
NucleoSpin 96 Food
Technology
Silica membrane
Format
8-well strips
96-well plates
Processing
Manual or automated, vacuum or centrifugation
Starting material
Up to 200 mg food or feed
Fragment size
300 bp–>30 kb
Typical yield
0.1–10 µg
A260/280
1.8–1.9
1.8–1.9
Elution volume
100–200 µl
Preparation time
60 min/6 strips (excl. lysis)
120 min/plate (excl. lysis)
Binding capacity 
30 µg
30 µg

Applications

  • Isolate DNA from complex matrices such as:
    • Processed food
    • Soy (milk and flour)
    • Chocolate
    • Cereals
    • Meat
    • Animal feed
  • Purified DNA is suitable for:
    • Real-time PCR
    • Southern blotting
    • Enzymatic reactions

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.