NucleoSpin 96 DNA FFPE—high-throughput DNA isolation from FFPE samples in a 96-well format
The NucleoSpin 96 DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples. The kit employs an odorless, xylene-free paraffin removal technology and a specialized decrosslinking buffer, enabling effective lysis in a convenient two-phase system. The kit protocol can be performed using centrifugation or vacuum processing, and can also be automated on common liquid-handling platforms.
The NucleoSpin 96 DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples. The kit employs an odorless, xylene-free paraffin removal technology and a specialized decrosslinking buffer, enabling effective lysis in a convenient two-phase system. The kit protocol can be performed using centrifugation or vacuum processing, and can also be automated on common liquid-handling platforms.
After paraffin is dissolved, samples are subjected to Proteinase K digestion to solubilize the fixed tissue and release DNA into solution. Subsequently, heat incubation with a specially designed buffer effectively eliminates crosslinks from the DNA, and large amounts of chaotropic salt and ethanol are added to the lysate to provide favorable conditions for binding of DNA to the silica membrane in the NucleoSpin 96 DNA FFPE Binding Plate. The binding process is reversible and specific to nucleic acids. While DNA is retained on the silica membrane, contaminants are removed by washing with two different wash buffers. Pure genomic DNA is eluted under low ionic strength conditions in a slightly alkaline elution buffer.
NucleoSpin 96 DNA FFPE procedure. The protocol can be performed using centrifugation or vacuum processing, and can be automated on common liquid-handling platforms
Overview
- Includes blue-colored, xylene-free Paraffin Dissolver for odorless paraffin removal
- Employs optimized decrosslinking buffer to eliminate formalin crosslinks
- Yields high-quality DNA suitable for downstream applications such as qPCR
- Allows for processing under vacuum or by centrifugation; protocol can be automated on common liquid-handling platforms
More Information
| Technology | Silica membrane |
| Format | 96-well plate |
| Processing | Manual or automated, vacuum or centrifugation |
| Starting material | ≤10 mg tissue ≤15 mg paraffin |
| Fragment size | 50 bp–approx. 5 kb |
| Typical yield | Depends on the amount and quality of the sample |
| A260/280 | Strongly depends on sample quality |
| Elution volume | 100 µl |
| Preparation time | ~60 min/plate (excl. lysis) |
| Binding capacity | 20 µg |
Applications
- High-throughput isolation of DNA from formalin-fixed, paraffin-embedded samples
- Isolation of DNA from fresh and archived FFPE samples
- Isolation of DNA from specimens on object slides
- Typical downstream application: PCR
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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