NucleoSpin 96 DNA FFPE—high-throughput DNA isolation from FFPE samples in a 96-well format

The NucleoSpin 96 DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples. The kit employs an odorless, xylene-free paraffin removal technology and a specialized decrosslinking buffer, enabling effective lysis in a convenient two-phase system. The kit protocol can be performed using centrifugation or vacuum processing, and can also be automated on common liquid-handling platforms.

After paraffin is dissolved, samples are subjected to Proteinase K digestion to solubilize the fixed tissue and release DNA into solution. Subsequently, heat incubation with a specially designed buffer effectively eliminates crosslinks from the DNA, and large amounts of chaotropic salt and ethanol are added to the lysate to provide favorable conditions for binding of DNA to the silica membrane in the NucleoSpin 96 DNA FFPE Binding Plate. The binding process is reversible and specific to nucleic acids. While DNA is retained on the silica membrane, contaminants are removed by washing with two different wash buffers. Pure genomic DNA is eluted under low ionic strength conditions in a slightly alkaline elution buffer.