Isolation of genomic DNA from tissue—NucleoSpin Tissue

Rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, and forensic samples

The NucleoSpin Tissue kits are designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, and forensic samples (e.g., hair, dried blood spots, buccal swabs, and cigarette stubs) and formalin-fixed, paraffin-embedded (FFPE) samples using mini spin columns. Up to 35 µg of high-purity genomic DNA can be prepared (typical yields from tissue or cells: 15–25 µg). Purified DNA can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.

With the NucleoSpin Tissue method, lysis is achieved by incubation of the samples in a solution containing SDS and Proteinase K at 56°C. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin Tissue columns are created by addition of large amounts of chaotropic ions and ethanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminants are removed by efficient washing with buffer. Pure genomic DNA is eluted under low ionic strength conditions in a slightly alkaline elution buffer, and is ready to use in subsequent reactions.

Genomic DNA isolation from extremely small tissue samples

The NucleoSpin Tissue XS kits are designed to provide maximum recovery and concentration of highly pure genomic DNA from "Xtra Small" (extremely dilute and low-volume) tissue samples, such as microdissected tissue, biopsies (e.g., fine needle aspiration), dried blood spots, buccal swabs, forensic samples, FFPE samples, and other small samples. NucleoSpin Tissue XS overcomes the limitations of conventional kits with an innovative column that is optimized for very small samples, allowing efficient elution in only 5–30 µl and resulting in highly concentrated DNA.

The NucleoSpin Tissue XS column contains a funnel-shaped thrust ring which holds a silica membrane of small diameter, allowing the accurate dispensing of small volumes on the membrane. NucleoSpin Tissue XS allows isolation of genomic DNA from samples as small as 0.025–10 mg of microdissected tissue, as little as 1–30 µl of fresh, frozen, or stabilized blood, or as few as 10–10,000 cells. The isolated DNA is free of impurities and suitable for sensitive downstream applications.

More Less

Cat. #	Product	Size	Price
740952.50	NucleoSpin® Tissue	50 Preps	$131.00
NucleoSpin Tissue (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections. Back The NucleoSpin Tissue procedure The NucleoSpin Tissue procedure.
740952.250	NucleoSpin® Tissue	250 Preps	$565.00
NucleoSpin Tissue (250) 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections. Back The NucleoSpin Tissue procedure The NucleoSpin Tissue procedure.
740952.240C	NucleoSpin® Tissue	240 Preps	$565.00
Kit for use on QIAcube. NucleoSpin Tissue (240) 240 preps for the isolation of genomic DNA from tissue Buffers, Proteinase K, NucleoSpin Tissue Columns Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like
740952.10	NucleoSpin® Tissue	Each	$29.00
NucleoSpin Tissue (10) 10 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components
740901.50	NucleoSpin® Tissue XS	50 Preps	$192.00
NucleoSpin Tissue XS (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.
740901.250	NucleoSpin® Tissue XS	250 Preps	$893.00
NucleoSpin Tissue XS (250) 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

Overview

NucleoSpin Tissue XS

High sensitivity for DNA isolation from small sample amounts
Elution in only 5 µl, resulting in concentrated DNA
Reliable DNA purification for reproducible results

NucleoSpin Tissue

High sensitivity
Reliable DNA purification for reproducible results
More than 16 support protocols included in the user manual for the greatest flexibility
Highest safety: spin columns can be closed—no cross-contamination
Cat. # 740952.240C is designed for use with the QIAcube only

More Information

	NucleoSpin Tissue XS	NucleoSpin Tissue
Technology	Silica membrane	Silica membrane
Format	XS spin columns	Mini spin columns
Starting material	Tissue samples: 0.025–10 mg Blood samples: 1–30 µl, fresh or frozen Cultured cells: 10–10,000 cells FFPE tissue: 0.001–10 mg	1–25 mg tissue 10²–10⁷ cultured cells
Fragment size	200 bp–>30 kb	200 bp–>30 kb
Typical yield	100 HeLa cells: 0.1–0.5 ng 1,000 HeLa cells: 1–5 ng 10,000 HeLa cells: 10–50 ng 0.025 mg mouse liver: 20–100 ng 0.25 mg mouse liver: 200–1,000 ng 2.5 mg mouse liver: 600–3,000 ng	20–35 µg
A_260/280	1.7–1.9	1.7–1.9
Elution volume	5–30 µl	60–100 µl
Preparation time	~40 min/prep (excl. lysis)	~20 min/prep (excl. lysis)

Applications

NucleoSpin Tissue XS
- DNA isolation from tissue (e.g., mouse kidney, laser micro-dissections)
- DNA isolation from cells (e.g., bacteria, yeast, cultured cells)
- DNA isolation from forensic samples (e.g., dried blood spots, buccal swabs)
- DNA isolation from FFPE tissue
- Obtained DNA is suitable for sensitive applications:
  - Multiplex PCR
  - Real-time PCR
NucleoSpin Tissue
- DNA from tissue (e.g., mouse tails)
- DNA from cells (e.g., cultured human or animal cells, bacteria, yeast)
- DNA from forensic samples (e.g., dried blood spots, hair, buccal swabs, cigarette stubs)
- DNA from FFPE tissue
- DNA from blood sample storage cards (e.g., NucleoSave)
- Obtained DNA suitable for:
  - Multiplex PCR
  - Southern blotting
  - Enzymatic reactions

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Purify DNA from small samples like microdissected tissue, small blood samples, or small amounts of cells.

Designed for extra small samples

Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.

Learn more All genomic DNA kits

25% off top-selling MN DNA and RNA purification kits

Huge savings on Macherey-Nagel mini/midi/maxi prep kits and more!

Stock up on top-selling MN Classics! Take advantage of great prices—save 25% and sweeten the deal with free Gummi Bears when you order one or more of the following:

This offer is valid in the U.S. and Canada only and expires 12.31.18. Limit one tub of Gummi Bears per order, while supplies last. Cite Discount Quote 31873 when ordering to receive the discount and free Gummi Bears. No other discounts apply.

Offer details Find other related kits

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2018 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.