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Rapid extraction of genomic DNA—NucleoSpin DNA RapidLyse

NucleoSpin DNA RapidLyse

The isolation of genomic DNA (gDNA) from most organs is a challenging task. Organs are composed of different tissues with various types of cells and matrices. Large molecules such as collagens and proteoglycans can impede lysis and inhibit downstream enzymatic reactions such as PCR. The new NucleoSpin DNA RapidLyse kit uses a unique lysis chemistry to release gDNA from organs and cells with the highest efficiency.

Cat. # Product Size Price License Quantity Details
740100.10 NucleoSpin® DNA RapidLyse 10 Preps USD $24.40

The NucleoSpin DNA RapidLyse kit is designed for fast and efficient isolation of genomic DNA from cells and organs like liver, kidney, heart, muscle, spleen, and lung. Processing of mouse tail and ear clippings is also possible. Fresh, frozen, and ethanol- preserved samples can be used.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740100.10: NucleoSpin DNA RapidLyse

740100.10: NucleoSpin DNA RapidLyse
740100.50 NucleoSpin® DNA RapidLyse 50 Preps USD $102.00

The NucleoSpin DNA RapidLyse kit is designed for fast and efficient isolation of genomic DNA from cells and organs like liver, kidney, heart, muscle, spleen, and lung. Processing of mouse tail and ear clippings is also possible. Fresh, frozen, and ethanol- preserved samples can be used.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740100.50: NucleoSpin DNA RapidLyse

740100.50: NucleoSpin DNA RapidLyse

Required Products

Cat. # Product Size Price License Quantity Details
740110.4 NucleoSpin® 96 DNA RapidLyse 4 Plates USD $890.00

The NucleoSpin DNA RapidLyse kit is designed for fast and efficient isolation of genomic DNA from cells and organs like liver, kidney, heart, muscle, spleen, and lung. Processing of mouse tail and ear clippings is also possible. Fresh, frozen, and ethanol- preserved samples can be used.

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740110.4: NucleoSpin 96 DNA RapidLyse

740110.4: NucleoSpin 96 DNA RapidLyse
740952.50 NucleoSpin® Tissue 50 Preps USD $133.00

NucleoSpin Tissue (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K

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Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections.

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The NucleoSpin Tissue procedure

The NucleoSpin Tissue procedure

The NucleoSpin Tissue procedure.

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740952.50: NucleoSpin Tissue

740952.50: NucleoSpin Tissue
740901.50 NucleoSpin® Tissue XS 50 Preps USD $195.00

NucleoSpin Tissue XS (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K

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NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

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740901.50: NucleoSpin Tissue XS

740901.50: NucleoSpin Tissue XS
740100.250 NucleoSpin® DNA RapidLyse 250 Preps USD $459.00

The NucleoSpin DNA RapidLyse kit is designed for fast and efficient isolation of genomic DNA from cells and organs like liver, kidney, heart, muscle, spleen, and lung. Processing of mouse tail and ear clippings is also possible. Fresh, frozen, and ethanol- preserved samples can be used.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740100.250: NucleoSpin DNA RapidLyse

740100.250: NucleoSpin DNA RapidLyse

Overview

• Unique lysis chemistry efficiently releases gDNA from cells, tissues, and organs
• Effective lysis in 5–60 min
• Superior gDNA yields compared to standard extraction methods

More Information

Technology Silica-membrane technology
Format Mini spin columns
Starting material
Fresh, frozen, dried, or ethanol-preserved organs
Eukaryotic cells
Tail or ear clippings
Sample amount
Up to 40 mg fresh weight, 1 x 106 cells
Typical yield Up to 4 µg of DNA per mg tissue
A260/280
1.7–1.9
Elution volume
60–100 µl
Lysis time
5–60 min
Preparation time 25 min/6 preps (excluding lysis)
Binding capacity 60 µg

Applications

  • Rapid isolation of genomic DNA from tail and ear clippings, organs, and cells

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Purify DNA from small samples like microdissected tissue, small blood samples, or small amounts of cells.

Designed for extra small samples

Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.

Learn more All genomic DNA kits

25% off top-selling MN DNA and RNA purification kits

Huge savings on best-selling DNA and RNA purification kits!

Stock up on top-selling MN Classics! Take advantage of great prices—save 25% and sweeten the deal with free Gummi Bears when you order one or more of the following:

  • NucleoSpin Gel and PCR Clean-up
  • NucleoSpin Plasmid/Plasmid Transfection-grade
  • NucleoSnap Plasmid Midi
  • NucleoBond Xtra Midi (Plus)/Xtra Maxi (Plus) 
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  • NucleoSpin DNA RapidLyse
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  • NucleoZOL/NucleoSpin RNA Set for NucleoZOL

This offer is valid in the U.S. and Canada only and expires 12.31.19. Limit one tub of Gummi Bears per order, while supplies last. Cite Discount Quote 36062 when ordering to receive the discount and free Gummi Bears. No other discounts apply.

Offer details Find other related kits

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