Rapid extraction of genomic DNA—NucleoSpin DNA RapidLyse

The isolation of genomic DNA (gDNA) from most organs is a challenging task. Organs are composed of different tissues with various types of cells and matrices. Large molecules such as collagens and proteoglycans can impede lysis and inhibit downstream enzymatic reactions such as PCR. The new NucleoSpin DNA RapidLyse kit uses a unique lysis chemistry to release gDNA from organs and cells with the highest efficiency.

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Product

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Price

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Quantity

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740100.10

NucleoSpin® DNA RapidLyse

10 Preps

$24.40

The NucleoSpin DNA RapidLyse kit is designed for fast and efficient isolation of genomic DNA from cells and organs like liver, kidney, heart, muscle, spleen, and lung. Processing of mouse tail and ear clippings is also possible. Fresh, frozen, and ethanol- preserved samples can be used.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

740100.50

NucleoSpin® DNA RapidLyse

50 Preps

$102.00

Notice to purchaser

Required Products

Cat. #	Product	Size	Price
740110.4	NucleoSpin® 96 DNA RapidLyse	4 Plates	$890.00
The NucleoSpin DNA RapidLyse kit is designed for fast and efficient isolation of genomic DNA from cells and organs like liver, kidney, heart, muscle, spleen, and lung. Processing of mouse tail and ear clippings is also possible. Fresh, frozen, and ethanol- preserved samples can be used. Documents You May Also Like Image Data Back 740110.4: NucleoSpin 96 DNA RapidLyse
740952.50	NucleoSpin® Tissue	50 Preps	$133.00
NucleoSpin Tissue (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K Documents You May Also Like Image Data Back Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections. Back The NucleoSpin Tissue procedure The NucleoSpin Tissue procedure.
740901.50	NucleoSpin® Tissue XS	50 Preps	$195.00
NucleoSpin Tissue XS (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K Documents You May Also Like Image Data Back NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

740100.250

NucleoSpin® DNA RapidLyse

250 Preps

$459.00

Notice to purchaser

Overview

• Unique lysis chemistry efficiently releases gDNA from cells, tissues, and organs
• Effective lysis in 5–60 min
• Superior gDNA yields compared to standard extraction methods

More Information

Technology	Silica-membrane technology
Format	Mini spin columns
Starting material	Fresh, frozen, dried, or ethanol-preserved organs Eukaryotic cells Tail or ear clippings
Sample amount	Up to 40 mg fresh weight, 1 x 10⁶ cells
Typical yield	Up to 4 µg of DNA per mg tissue
A_260/280	1.7–1.9
Elution volume	60–100 µl
Lysis time	5–60 min
Preparation time	25 min/6 preps (excluding lysis)
Binding capacity	60 µg

Applications

Rapid isolation of genomic DNA from tail and ear clippings, organs, and cells

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Purify DNA from small samples like microdissected tissue, small blood samples, or small amounts of cells.

Designed for extra small samples

Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.

Learn more All genomic DNA kits

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.