Rapid extraction of genomic DNA—NucleoSpin DNA RapidLyse
The isolation of genomic DNA (gDNA) from most organs is a challenging task. Organs are composed of different tissues with various types of cells and matrices. Large molecules such as collagens and proteoglycans can impede lysis and inhibit downstream enzymatic reactions such as PCR. The new NucleoSpin DNA RapidLyse kit uses a unique lysis chemistry to release gDNA from organs and cells with the highest efficiency.
Overview
• Unique lysis chemistry efficiently releases gDNA from cells, tissues, and organs
• Effective lysis in 5–60 min
• Superior gDNA yields compared to standard extraction methods
More Information
| Technology | Silica-membrane technology |
| Format | Mini spin columns |
| Starting material |
Fresh, frozen, dried, or ethanol-preserved organs Eukaryotic cells Tail or ear clippings |
| Sample amount |
Up to 40 mg fresh weight, 1 x 106 cells |
| Typical yield | Up to 4 µg of DNA per mg tissue |
| A260/280 |
1.7–1.9 |
| Elution volume |
60–100 µl |
| Lysis time |
5–60 min |
| Preparation time | 25 min/6 preps (excluding lysis) |
| Binding capacity | 60 µg |
Applications
- Rapid isolation of genomic DNA from tail and ear clippings, organs, and cells
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
Learn more All genomic DNA kitsTakara Bio USA, Inc.
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