NucleoMag DNA Bacteria
The NucleoMag DNA Bacteria kit enables high-throughput DNA isolation from diverse microbial samples using scalable, automation-friendly magnetic beads. The kit can be used to process Gram-positive and Gram-negative bacteria, as well as yeast and other fungi. Common sample types include microbial cultures employed for industrial applications such as food research and chemical production, or organisms relevant to clinical and ecological studies. Typical downstream applications for DNA purified with the kit include PCR, qPCR, and NGS.
The NucleoMag DNA Bacteria kit enables high-throughput DNA isolation from diverse microbial samples using scalable, automation-friendly magnetic beads. The kit can be used to process Gram-positive and Gram-negative bacteria, as well as yeast and other fungi. Common sample types include microbial cultures employed for industrial applications such as food research and chemical production, or organisms relevant to clinical and ecological studies. Typical downstream applications for DNA purified with the kit include PCR, qPCR, and NGS.
NucleoMag DNA Bacteria utilizes a powerful yet environmentally friendly buffer chemistry that is free of chaotropic salts or hazardous components. The kit can be combined with bead-based homogenization approaches (e.g., MN bead tubes or MN 96 bead plates) for improved sample disruption, and support protocols are provided for DNA extraction from hard-shelled organisms (e.g., insects and crustaceans), lipid rich, and fungal samples.
Overview
- Suitable for high-throughput DNA extraction from a diverse array of microbes, including Gram-negative and Gram-positive bacteria and fungi
- Scalable magnetic bead-based purification technology can be easily automated on common liquid handling platforms
- Environmentally friendly buffer chemistry avoids the use of chaotropic salts and hazardous materials
- Liquid Proteinase K and Liquid RNase A provided for easy handling
- Can be combined with MN bead tubes or MN 96 bead plates for more efficient sample disruption
More Information
| Technology | Magnetic-bead technology |
| Processing | Manual or automated |
| Sample material | Cell culture pellets of Gram-positive or Gram-negative bacteria, yeasts, or other fungi |
| Sample amount | Up to approx. 40 mg wet weight |
| Sample types tested |
Bacteria: E. coli, B. subtilis, C. glutamicum, V. fischeri |
| Elution volume | 50–200 µl |
| Preparation time | 30 min on KingFisher Flex (excluding sample lysis) |
| Theoretical binding capacity | 0.4 µg/µl beads |
*Support protocol available. Please contact technical support.
Applications
- Isolation of DNA from microbial cell cultures
- Typical downstream applications: PCR, qPCR, NGS
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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