Isolation of genomic DNA from food & feed using magnetic beads—NucleoMag DNA Food
The NucleoMag DNA Food kit is designed for rapid manual and automated small-scale preparation of DNA from <200 mg food or feed samples of plant or animal origin, with typical yields ranging from 0.1–10 μg of pure DNA. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions.
The NucleoMag DNA Food kit is designed for rapid manual and automated small-scale preparation of DNA from <200 mg food or feed samples of plant or animal origin, with typical yields ranging from 0.1–10 μg of pure DNA. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Magnetic separation can be performed either manually using the NucleoMag SEP magnetic separator or automated on standard liquid handling instruments and automated magnetic separators. Expand the "Details" section of the product table below and select "Image Data" to view data generated with the kit using various food types as inputs.
Overview
- Purify DNA from food and feed for species identification and GMO detection
- Isolate even small amounts of partially degraded DNA from complex matrices
- Extract DNA from contaminating bacteria
- Process a wide variety of samples using flexible kit chemistry
More Information
| Technology | Magnetic beads |
| Format | Highly reactive superparamagnetic beads |
| Processing | Manual or automated |
| Starting material | <200 mg food or feed |
| Fragment size | 300 bp–approx. 50 kb |
| Typical yield | 0.1–10 μg |
| A260/A280 | 1.6–1.9 |
| Elution volume | 50–200 µl |
| Preparation time | 120 min/96 preps (excl. lysis) |
| Binding capacity | 0.4 µg/µl beads |
Applications
- Manual or automated isolation of DNA from food and feed
- Identification of GMO DNA or animal components
- Extraction of DNA from contaminating bacteria
- Typical downstream application: real-time PCR
RNA and DNA isolation from pathogens
The NucleoMag Pathogen kit is designed for the rapid manual and automated small-scale isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes.
NucleoMag Pathogen NucleoMag SEP magnetic separatorTakara Bio USA, Inc.
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