NucleoBond CB
NucleoBond CB kits are designed for the purification of genomic DNA from blood, serum, or mammalian cell cultures using NucleoBond AXG anion-exchange columns, which yield ultra-pure high-molecular-weight genomic DNA. Because NucleoBond columns run by gravity flow, shearing is kept to a minimum so that high-molecular-weight genomic DNA can be easily isolated.
Samples are first lysed under special conditions for removal of erythrocytes.
NucleoBond CB kits are designed for the purification of genomic DNA from blood, serum, or mammalian cell cultures using NucleoBond AXG anion-exchange columns, which yield ultra-pure high-molecular-weight genomic DNA. Because NucleoBond columns run by gravity flow, shearing is kept to a minimum so that high-molecular-weight genomic DNA can be easily isolated.
Samples are first lysed under special conditions for removal of erythrocytes. After incubation with Proteinase K, the sample is diluted with Buffer N2 before loading onto the NucleoBond AXG column in order to guarantee a good flow rate as well as quantitative binding of the DNA. After elution with high-salt buffer, the genomic DNA is precipitated with isopropanol.
Overview
- Ultra-pure genomic DNA from different samples
- Kits for 100–500 μg of genomic DNA
- Sample size: up to 20 ml of whole blood
- Isolate high-molecular-weight DNA (up to 300 kb)
More Information
| NucleoBond CB 100 | NucleoBond CB 500 | |
| Technology | Anion-exchange chromatography | |
| Format | Midi gravity-flow columns | Maxi gravity-flow columns |
| Starting material | 2–5 ml whole blood <250 µl buffy coat 2 x 107 cultured cells |
5–20 ml whole blood <1 ml buffy coat 108 cultured cells |
| Fragment size | 500 bp→300 kb | |
| Typical yield | 100 µg | 500 µg |
| A260/280 | 1.80–1.95 | |
| Preparation time | 4–5 hours | 4–5 hours |
| Binding capacity | 100 µg | 500 µg |
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Learn more All genomic DNA kitsTakara Bio USA, Inc.
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