NucleoSpin DNA Yeast

The NucleoSpin DNA Yeast kit enables efficient isolation of genomic DNA from pelleted yeast samples. By incorporating a mechanical lysis step with corundum beads, the kit provides a faster protocol than established methods while yielding up to 20 µg of DNA per prep. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris.

Preliminary data also indicate the usability of the kit for DNA isolation from fungal mycelia (e.g., A. nidulans), from bacterial spore suspensions (e.g., G. stearothermophilus), and from plant pollen (e.g., honey bee pollen baskets). For optimal DNA yields, bead tubes different from the ones included in the kit may be required.

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Cat. #	Product	Size
740236.10	NucleoSpin® DNA Yeast	10 Preps	USD $62.00
NucleoSpin DNA Yeast enables efficient isolation of genomic DNA from a diverse array of yeast samples, yielding up to 20 µg of DNA per prep. In contrast with other extraction methods that involve chemical lysis or sample disruption with glass beads, NucleoSpin DNA Yeast employs corundum homogenization beads to enable more efficient sample lysis, resulting in a protocol that can be completed in 35 minutes. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris, and processing of additional sample types (e.g., fungal mycelia, bacterial spore suspensions, plant pollen) is also possible. Cat. # 740236.10 includes reagents and materials for processing 10 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Higher DNA yields from yeast using MN Bead Tubes Type C Higher DNA yields from yeast using MN Bead Tubes Type C. Yeast samples (S. cerevisiae, 40-mg input) were subjected to mechanical lysis using several different methods and processed using the NucleoSpin DNA Yeast kit. Mechanical lysis using MN Bead Tubes Type C provided the highest yields of all methods tested. Back High DNA yields from yeast sample inputs up to 100 mg High DNA yields from yeast sample inputs up to 100 mg. Genomic DNA was isolated from varying inputs (wet weight) of pelleted yeast (S. cerevisiae) using NucleoSpin DNA Yeast. As demonstrated, the kit yielded proportionally higher amounts of DNA for sample inputs up 100 mg. Back 740236.10: NucleoSpin DNA Yeast
740236.50	NucleoSpin® DNA Yeast	50 Preps	USD $220.00
NucleoSpin DNA Yeast enables efficient isolation of genomic DNA from a diverse array of yeast samples, yielding up to 20 µg of DNA per prep. In contrast with other extraction methods that involve chemical lysis or sample disruption with glass beads, NucleoSpin DNA Yeast employs corundum homogenization beads to enable more efficient sample lysis, resulting in a protocol that can be completed in 35 minutes. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris, and processing of additional sample types (e.g., fungal mycelia, bacterial spore suspensions, plant pollen) is also possible. Cat. # 740236.50 includes reagents and materials for processing 50 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Higher DNA yields from yeast using MN Bead Tubes Type C Higher DNA yields from yeast using MN Bead Tubes Type C. Yeast samples (S. cerevisiae, 40-mg input) were subjected to mechanical lysis using several different methods and processed using the NucleoSpin DNA Yeast kit. Mechanical lysis using MN Bead Tubes Type C provided the highest yields of all methods tested. Back High DNA yields from yeast sample inputs up to 100 mg High DNA yields from yeast sample inputs up to 100 mg. Genomic DNA was isolated from varying inputs (wet weight) of pelleted yeast (S. cerevisiae) using NucleoSpin DNA Yeast. As demonstrated, the kit yielded proportionally higher amounts of DNA for sample inputs up 100 mg. Back 740236.50: NucleoSpin DNA Yeast

Overview

Processing of ≤100 mg input material per prep
Yields up to 20 µg of genomic DNA (depending on sample type)
Protocol can be completed in 35 min
Corundum homogenization beads included for efficient sample lysis

More Information

Technology	Silica membrane
Sample material	Yeast, fungi
Sample amount	≤100 mg (wet weight)
Fragment size	200 bp–approx. 50 kbp
Typical yield	Up to 20 µg (depending on sample amount and quality)
Elution volume	100–200 µl
Processing time	35 min/prep

Applications

Isolation of DNA from pelleted yeast samples
Typical downstream applications: PCR, qPCR, NGS

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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NucleoSpin DNA Yeast

Notice to purchaser

Higher DNA yields from yeast using MN Bead Tubes Type C

High DNA yields from yeast sample inputs up to 100 mg

740236.10: NucleoSpin DNA Yeast