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Home › Products › Nucleic acid purification › Genomic DNA purification kits › Other organisms and samples › NucleoSpin DNA Yeast

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NucleoSpin DNA Yeast

The NucleoSpin DNA Yeast kit enables efficient isolation of genomic DNA from pelleted yeast samples. By incorporating a mechanical lysis step with corundum beads, the kit provides a faster protocol than established methods while yielding up to 20 µg of DNA per prep. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris.

The NucleoSpin DNA Yeast kit enables efficient isolation of genomic DNA from pelleted yeast samples. By incorporating a mechanical lysis step with corundum beads, the kit provides a faster protocol than established methods while yielding up to 20 µg of DNA per prep. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris.

Preliminary data also indicate the usability of the kit for DNA isolation from fungal mycelia (e.g., A. nidulans), from bacterial spore suspensions (e.g., G. stearothermophilus), and from plant pollen (e.g., honey bee pollen baskets). For optimal DNA yields, bead tubes different from the ones included in the kit may be required.

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Cat. # Product Size License Quantity Details
740236.10 NucleoSpin® DNA Yeast 10 Preps USD $62.00

NucleoSpin DNA Yeast enables efficient isolation of genomic DNA from a diverse array of yeast samples, yielding up to 20 µg of DNA per prep. In contrast with other extraction methods that involve chemical lysis or sample disruption with glass beads, NucleoSpin DNA Yeast employs corundum homogenization beads to enable more efficient sample lysis, resulting in a protocol that can be completed in 35 minutes. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris, and processing of additional sample types (e.g., fungal mycelia, bacterial spore suspensions, plant pollen) is also possible.

Cat. # 740236.10 includes reagents and materials for processing 10 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Higher DNA yields from yeast using MN Bead Tubes Type C

Higher DNA yields from yeast using MN Bead Tubes Type C

Higher DNA yields from yeast using MN Bead Tubes Type C. Yeast samples (S. cerevisiae, 40-mg input) were subjected to mechanical lysis using several different methods and processed using the NucleoSpin DNA Yeast kit. Mechanical lysis using MN Bead Tubes Type C provided the highest yields of all methods tested.

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High DNA yields from yeast sample inputs up to 100 mg

High DNA yields from yeast sample inputs up to 100 mg

High DNA yields from yeast sample inputs up to 100 mg. Genomic DNA was isolated from varying inputs (wet weight) of pelleted yeast (S. cerevisiae) using NucleoSpin DNA Yeast. As demonstrated, the kit yielded proportionally higher amounts of DNA for sample inputs up 100 mg.

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740236.10: NucleoSpin DNA Yeast

740236.10: NucleoSpin DNA Yeast
740236.50 NucleoSpin® DNA Yeast 50 Preps USD $220.00

NucleoSpin DNA Yeast enables efficient isolation of genomic DNA from a diverse array of yeast samples, yielding up to 20 µg of DNA per prep. In contrast with other extraction methods that involve chemical lysis or sample disruption with glass beads, NucleoSpin DNA Yeast employs corundum homogenization beads to enable more efficient sample lysis, resulting in a protocol that can be completed in 35 minutes. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris, and processing of additional sample types (e.g., fungal mycelia, bacterial spore suspensions, plant pollen) is also possible.

Cat. # 740236.50 includes reagents and materials for processing 50 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

Higher DNA yields from yeast using MN Bead Tubes Type C

Higher DNA yields from yeast using MN Bead Tubes Type C

Higher DNA yields from yeast using MN Bead Tubes Type C. Yeast samples (S. cerevisiae, 40-mg input) were subjected to mechanical lysis using several different methods and processed using the NucleoSpin DNA Yeast kit. Mechanical lysis using MN Bead Tubes Type C provided the highest yields of all methods tested.

Back

High DNA yields from yeast sample inputs up to 100 mg

High DNA yields from yeast sample inputs up to 100 mg

High DNA yields from yeast sample inputs up to 100 mg. Genomic DNA was isolated from varying inputs (wet weight) of pelleted yeast (S. cerevisiae) using NucleoSpin DNA Yeast. As demonstrated, the kit yielded proportionally higher amounts of DNA for sample inputs up 100 mg.

Back

740236.50: NucleoSpin DNA Yeast

740236.50: NucleoSpin DNA Yeast

Homogenization bead selection guide
Yeast culture media including YPD, YPDA, minimal media, and amino acid dropout mixes Yeast media
NucleoSpin Microbial DNA product page NucleoSpin Microbial DNA product page

Overview

  • Processing of ≤100 mg input material per prep
  • Yields up to 20 µg of genomic DNA (depending on sample type)
  • Protocol can be completed in 35 min
  • Corundum homogenization beads included for efficient sample lysis

More Information

Technology Silica membrane
Sample material Yeast, fungi
Sample amount ≤100 mg (wet weight)
Fragment size 200 bp–approx. 50 kbp
Typical yield Up to 20 µg (depending on sample amount and quality)
Elution volume 100–200 µl
Processing time 35 min/prep

Applications

  • Isolation of DNA from pelleted yeast samples
  • Typical downstream applications: PCR, qPCR, NGS

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
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  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
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  • Purified total RNA and mRNA
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  • Most popular polymerases
  • High-yield PCR
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  • GC rich PCR
  • PCR master mixes
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  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
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  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
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