NucleoSpin DNA Yeast
The NucleoSpin DNA Yeast kit enables efficient isolation of genomic DNA from pelleted yeast samples. By incorporating a mechanical lysis step with corundum beads, the kit provides a faster protocol than established methods while yielding up to 20 µg of DNA per prep. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris.
The NucleoSpin DNA Yeast kit enables efficient isolation of genomic DNA from pelleted yeast samples. By incorporating a mechanical lysis step with corundum beads, the kit provides a faster protocol than established methods while yielding up to 20 µg of DNA per prep. The kit has been confirmed to perform well with a variety of popular yeast strains, including S. cerevisiae, S. pombe, and P. pastoris.
Preliminary data also indicate the usability of the kit for DNA isolation from fungal mycelia (e.g., A. nidulans), from bacterial spore suspensions (e.g., G. stearothermophilus), and from plant pollen (e.g., honey bee pollen baskets). For optimal DNA yields, bead tubes different from the ones included in the kit may be required.
Overview
- Processing of ≤100 mg input material per prep
- Yields up to 20 µg of genomic DNA (depending on sample type)
- Protocol can be completed in 35 min
- Corundum homogenization beads included for efficient sample lysis
More Information
| Technology | Silica membrane |
| Sample material | Yeast, fungi |
| Sample amount | ≤100 mg (wet weight) |
| Fragment size | 200 bp–approx. 50 kbp |
| Typical yield | Up to 20 µg (depending on sample amount and quality) |
| Elution volume | 100–200 µl |
| Processing time | 35 min/prep |
Applications
- Isolation of DNA from pelleted yeast samples
- Typical downstream applications: PCR, qPCR, NGS
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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