Isolation of genomic DNA from tissues—NucleoBond AXG
NucleoBond AXG columns are anion-exchange columns for isolating ultra-pure genomic DNA from tissue, bacteria, or yeast. Macherey-Nagel offers buffer sets for extracting genomic DNA from tissue and bacteria, which include all required buffers and enzymes (Proteinase K and RNase A). Using these buffer sets in combination with NucleoBond AXG columns allows isolation of up to 500 µg of genomic DNA from different matrices.
NucleoBond AXG columns are anion-exchange columns for isolating ultra-pure genomic DNA from tissue, bacteria, or yeast. Macherey-Nagel offers buffer sets for extracting genomic DNA from tissue and bacteria, which include all required buffers and enzymes (Proteinase K and RNase A). Using these buffer sets in combination with NucleoBond AXG columns allows isolation of up to 500 µg of genomic DNA from different matrices. Isolation of genomic DNA from yeast requires additional buffers (see user manual). NucleoBond AXG columns are available in three different sizes (AXG 20, AXG 100, and AXG 500).
The quality of the isolated genomic DNA is extraordinarily high. AXG columns used to purify genomic DNA with a gravity flow-based protocol yield high molecular weight DNA without shearing. Samples are first lysed with Proteinase K. Before loading, the sample is diluted with Buffer N2 in order to guarantee a good flow rate as well as quantitative DNA binding. After efficient washing, the genomic DNA is eluted with high-salt buffer and precipitated with isopropanol. The DNA purified using this method is suitable for all kinds of subsequent reactions.
Overview
- Ultra-pure genomic DNA from from tissue, bacteria, or yeast
- Combine NucleoBond AXG columns and the corresponding buffer set for highest flexibility
- NucleoBond AXG columns + NucleoBond Buffer Set III for isolation of genomic DNA from bacteria and yeast
- NucleoBond AXG columns + NucleoBond Buffer Set IV for isolation of genomic DNA from tissue
More Information
| NucleoBond AXG 20 | NucleoBond AXG 100 | NucleoBond AXG 500 | |
| Technology | Anion-exchange technology | ||
| Format | Mini gravity-flow columns | Midi gravity-flow columns | Maxi gravity-flow columns |
| Starting material | 2–5 ml bacterial culture 3–5 ml yeast culture <20 mg tissue |
15–20 ml bacterial culture 10–20 ml yeast culture <100 mg tissue |
60–80 ml bacterial culture 100–500 ml yeast culture <400 mg tissue |
| Fragment size | 500 bp–300 kb | 500 bp–300 kb | 500 bp–300 kb |
| Typical yield | 20 µg | 100 µg | 500 µg |
| A260/280 | 1.80–1.95 | 1.80–1.95 | 1.80–1.95 |
| Preparation time | 4–5 hr | 4–5 hr | 4–5 hr |
| Binding capacity | 20 µg | 100 µg | 500 µg |
Applications
- Isolation of genomic DNA from bacteria, yeast, and tissue
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
Learn more All genomic DNA kitsTakara Bio USA, Inc.
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