NucleoMag HMW DNA
NucleoMag HMW DNA enables magnetic bead-based isolation of high-quality, high-molecular-weight (HMW) DNA for long-range sequencing. The kit is compatible with a wide range of sample materials, including cells, human and animal tissue, plant tissue, whole blood, saliva, swabs, bacteria, and yeast. The workflow can be automated on liquid handling platforms in combination with NucleoMag SEP and on magnetic rod systems such as the IsoPure Mini.
Overview
- Isolate HMW DNA (up to 200 kb) via magnetic bead-based technology
- Obtain high DNA yield and purity from a wide range of sample materials
- Process samples on an automated platform or manually
More Information
| Format | Magnetic beads |
| Fragment Size | From 50–200 kb |
| Typical yield | Depends on sample type and amount |
| Sample materials | Animal tissue, bacteria, blood, cells, human tissue, plant, saliva, yeast |
| Sample amounts for select sample materials | ≤1 x 10⁶ cultured cells ≤20 mg human/animal tissue ≤50 mg plant leaves ≤25 mg gram-negative bacteria ≤35 mg gram-positive bacteria ≤50 mg yeast |
| Automated platform compatibility | Compatible with most open robotic platforms. Please contact technical support for more information. |
| Elution volume | 100–200 µl |
| Processing time | 60–120 min, depending on manual or automated processing |
Applications
- Isolation of high-molecular-weight DNA from mammalian tissues, blood samples, invertebrates, plants, bacteria, and yeast
- Typical downstream applications: long-read sequencing (e.g., SMRT, Nanopore, Next GEM)
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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