High-throughput plant DNA isolation in a 384-well format—NucleoMag 384 Plant
The NucleoMag 384 Plant kit employs a magnetic bead-based purification technology that enables rapid and efficient isolation of genomic DNA from a wide array of plant species and tissue types and allows for processing of up to 384 samples in parallel. The kit protocol can be automated on liquid-handling platforms and is particularly well suited for high-throughput applications such as plant breeding or population-level analyses.
The NucleoMag 384 Plant kit employs a magnetic bead-based purification technology that enables rapid and efficient isolation of genomic DNA from a wide array of plant species and tissue types and allows for processing of up to 384 samples in parallel. The kit protocol can be automated on liquid-handling platforms and is particularly well suited for high-throughput applications such as plant breeding or population-level analyses.
The NucleoMag 384 Plant kit accommodates up to 30 mg of sample material per prep and is designed to overcome common challenges associated with plant DNA purification, including the release of polyphenolic compounds and complex polysaccharides. Plant tissue is extracted with CTAB Lysis Buffer MC1, and binding of genomic DNA to the NucleoMag V-Beads is facilitated by Binding Buffer MC2. Following magnetic separation and removal of supernatant, the paramagnetic beads are washed in a series of buffers to remove contaminants, salt, and residual ethanol. Following elution under low-salt conditions, isolated genomic DNA is ready for downstream applications such as NGS, qPCR, blotting, or enzymatic reactions.
Overview
- Optimized for high-throughput, automated processing in a 384-well format
- Enables efficient DNA purification from up to 30 mg of sample material per prep
- Suitable for a wide array of plant species and tissue types
- Purified DNA is ready for downstream applications such as qPCR and NGS
More Information
| Technology | Magnetic bead technology |
| Format | Highly reactive superparamagnetic beads |
| Processing | Manual or automated |
| Starting material | ≤30 mg plant tissue (wet weight) |
| Plant species successfully tested | Zea mays (maize), Brassica napus (rapeseed), Glycine max (soy), Triticum aestivum (wheat), Nicotiana tabacum (tobacco), Elaeis guineensis (oil palm) |
| Typical yield |
Depends on sample source, storage, and quality |
| A260/A280 | 1.6–1.9 |
| Elution volume |
40–100 µl |
| Preparation time | 60 min/384 preps* |
| Binding capacity | 0.2 μg/μl beads |
*Using TECAN Freedom EVO MCA 384
Applications
- Manual or automated isolation of DNA from plant tissue
- Typical downstream applications: PCR, Southern blotting, any kind of enzymatic reaction
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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