Isolation of genomic DNA from FFPE samples using magnetic beads—NucleoMag DNA FFPE

NucleoMag DNA FFPE allows rapid, high-throughput DNA purification from formalin-fixed, paraffin-embedded samples by reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. It includes the odorless Paraffin Dissolver for effective lysis in a convenient two-phase system. Magnetic separation can be performed manually using the NucleoMag SEP magnetic separator, or with automated magnetic separators and standard liquid handling instruments.

Cat. #	Product	Size	Price
744320.1	NucleoMag® DNA FFPE	1 x 96 Preps	USD $505.00
NucleoMag DNA FFPE kits employ superparamagnetic beads to enable high-throughput DNA purification from formalin-fixed, paraffin-embedded (FFPE) samples using manual or automated processing. The kits can be used to process fresh or archived samples and are compatible with inputs of ≤5 mg of tissue and ≤15 mg of paraffin. Purified DNA fragments typically range in size from 50 bp–5 kbp and are eluted in volumes >25 µl. The kits include blue-colored Paraffin Dissolver for convenient paraffin removal and enable processing of 96 samples in ~2 hours. Cat. # 744320.1 includes sufficient reagents and materials for processing of 96 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Competitor A (A) and competitor I (B) show higher CT values, indicating a lower yield compared to samples purified with NucleoMag DNA FFPE. NucleoMag DNA FFPE provides better PCR performance than competitor kits. Genomic DNA was purified from FFPE samples using NucleoMag DNA FFPE in comparison with kits from Competitor A and Competitor I and analyzed using a LightCycler PCR system and the DyNAmo Capillary SYBR Green qPCR Kit (amplicon size: 191 bp). qPCR of DNA purified with kits from Competitor A (Panel A) and Competitor I (Panel B) resulted in higher Ct values, indicating lower DNA yields compared to samples purified with NucleoMag DNA FFPE. Back The NucleoMag DNA FFPE kit leverages the reversible adsorption of nucleic acids on paramagnetic beads to allow a quick, simple workflow for high-purity genomic DNA from FFPE tissue. The NucleoMag DNA FFPE procedure. Back The NucleoMag DNA FFPE kit provides higher DNA yields from embedded mouse lung, liver, and kidney sections compared to competitor kits (A, I). NucleoMag DNA FFPE provides higher yields than competitor kits. Genomic DNA was purified in triplicate from embedded mouse lung, liver, and kidney tissue sections using NucleoMag DNA FFPE and two competitor kits. The NucleoMag DNA FFPE kit provided higher DNA yields than the competitor kits (A, I). Back 744320.1: NucleoMag DNA FFPE
744320.4	NucleoMag® DNA FFPE	4 x 96 Preps	USD $1755.00
NucleoMag DNA FFPE kits employ superparamagnetic beads to enable high-throughput DNA purification from formalin-fixed, paraffin-embedded (FFPE) samples using manual or automated processing. The kits can be used to process fresh or archived samples and are compatible with inputs of ≤5 mg of tissue and ≤15 mg of paraffin. Purified DNA fragments typically range in size from 50 bp–5 kbp and are eluted in volumes >25 µl. The kits include blue-colored Paraffin Dissolver for convenient paraffin removal and enable processing of 96 samples in ~2 hours. Cat. # 744320.4 includes sufficient reagents and materials for processing of 4 x 96 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Competitor A (A) and competitor I (B) show higher CT values, indicating a lower yield compared to samples purified with NucleoMag DNA FFPE. NucleoMag DNA FFPE provides better PCR performance than competitor kits. Genomic DNA was purified from FFPE samples using NucleoMag DNA FFPE in comparison with kits from Competitor A and Competitor I and analyzed using a LightCycler PCR system and the DyNAmo Capillary SYBR Green qPCR Kit (amplicon size: 191 bp). qPCR of DNA purified with kits from Competitor A (Panel A) and Competitor I (Panel B) resulted in higher Ct values, indicating lower DNA yields compared to samples purified with NucleoMag DNA FFPE. Back The NucleoMag DNA FFPE kit leverages the reversible adsorption of nucleic acids on paramagnetic beads to allow a quick, simple workflow for high-purity genomic DNA from FFPE tissue. The NucleoMag DNA FFPE procedure. Back The NucleoMag DNA FFPE kit provides higher DNA yields from embedded mouse lung, liver, and kidney sections compared to competitor kits (A, I). NucleoMag DNA FFPE provides higher yields than competitor kits. Genomic DNA was purified in triplicate from embedded mouse lung, liver, and kidney tissue sections using NucleoMag DNA FFPE and two competitor kits. The NucleoMag DNA FFPE kit provided higher DNA yields than the competitor kits (A, I). Back 744320.4: NucleoMag DNA FFPE
740968.25	Paraffin Dissolver	25 mL	USD $37.00
Paraffin Dissolver is an odorless, xylene-free reagent that enables efficient sample deparaffinization and is included with corresponding NucleoSpin and NucleoMag kits for purification of DNA or RNA from FFPE tissues. Cat. # 740968.25 consists of a bottle containing 25 ml of Paraffin Dissolver reagent. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740968.25: Paraffin Dissolver
740343.60	Paraffin Dissolver (blue)	60 mL	USD $78.00
Paraffin Dissolver (blue) is an odorless, xylene-free reagent that enables efficient sample deparaffinization for purification of DNA or RNA from FFPE tissues. Cat. # 740343.60 consists of a bottle containing 60 ml of Paraffin Dissolver (blue) reagent. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740343.60: Paraffin Dissolver (blue)

Overview

Blue-colored Paraffin Dissolver for convenient paraffin removal
Removal of formalin crosslinking by efficient decrosslinking buffer
High-quality DNA for improved performance in downstream applications (e.g., PCR)
Magnetic bead technology allows highest flexibility, easy adaption for automated use
Support protocol for RNA extraction from FFPE samples also available

More Information

Technology	Magnetic beads
Format	Highly reactive superparamagnetic beads
Processing	Manual or automated
Starting material	≤5 mg tissue, ≤15 mg paraffin
Fragment size	50 bp–approx. 5 kb
Typical yield	Depends on amount and quality of sample
A_260/280	Strongly depends on sample quality
Elution volume	>25 µl
Preparation time	120 min/96 preps (excluding lysis)
Binding capacity	0.4 µg/µl

Applications

Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
Isolation of DNA from fresh and archived FFPE samples
Isolation of DNA from specimen on object slides
Typical downstream application: PCR
RNA extraction from FFPE samples using available support protocol

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

High-throughput RNA isolation from FFPE samples

Access a protocol for high-throughput RNA isolation from FFPE samples using the NucleoMag DNA FFPE kit.

Access protocol

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Need help automating your nucleic acid purification workflow?

Isolation of genomic DNA from FFPE samples using magnetic beads—NucleoMag DNA FFPE

Notice to purchaser

Competitor A (A) and competitor I (B) show higher CT values, indicating a lower yield compared to samples purified with NucleoMag DNA FFPE.

The NucleoMag DNA FFPE kit leverages the reversible adsorption of nucleic acids on paramagnetic beads to allow a quick, simple workflow for high-purity genomic DNA from FFPE tissue.

The NucleoMag DNA FFPE kit provides higher DNA yields from embedded mouse lung, liver, and kidney sections compared to competitor kits (A, I).

744320.1: NucleoMag DNA FFPE

Notice to purchaser

Competitor A (A) and competitor I (B) show higher CT values, indicating a lower yield compared to samples purified with NucleoMag DNA FFPE.

The NucleoMag DNA FFPE kit leverages the reversible adsorption of nucleic acids on paramagnetic beads to allow a quick, simple workflow for high-purity genomic DNA from FFPE tissue.

The NucleoMag DNA FFPE kit provides higher DNA yields from embedded mouse lung, liver, and kidney sections compared to competitor kits (A, I).

744320.4: NucleoMag DNA FFPE

Notice to purchaser

740968.25: Paraffin Dissolver

Notice to purchaser

740343.60: Paraffin Dissolver (blue)

Overview

More Information

Applications

Additional product information