Isolation of genomic DNA from FFPE samples using magnetic beads—NucleoMag DNA FFPE
NucleoMag DNA FFPE allows rapid, high-throughput DNA purification from formalin-fixed, paraffin-embedded samples by reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. It includes the odorless Paraffin Dissolver for effective lysis in a convenient two-phase system. Magnetic separation can be performed manually using the NucleoMag SEP magnetic separator, or with automated magnetic separators and standard liquid handling instruments.
Overview
- Blue-colored Paraffin Dissolver for convenient paraffin removal
- Removal of formalin crosslinking by efficient decrosslinking buffer
- High-quality DNA for improved performance in downstream applications (e.g., PCR)
- Magnetic bead technology allows highest flexibility, easy adaption for automated use
- Support protocol for RNA extraction from FFPE samples also available
More Information
| Technology | Magnetic beads |
| Format | Highly reactive superparamagnetic beads |
| Processing | Manual or automated |
| Starting material | ≤5 mg tissue, ≤15 mg paraffin |
| Fragment size | 50 bp–approx. 5 kb |
| Typical yield | Depends on amount and quality of sample |
| A260/280 | Strongly depends on sample quality |
| Elution volume | >25 µl |
| Preparation time | 120 min/96 preps (excluding lysis) |
| Binding capacity | 0.4 µg/µl |
Applications
- Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
- Isolation of DNA from fresh and archived FFPE samples
- Isolation of DNA from specimen on object slides
- Typical downstream application: PCR
- RNA extraction from FFPE samples using available support protocol
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
High-throughput RNA isolation from FFPE samples
Access a protocol for high-throughput RNA isolation from FFPE samples using the NucleoMag DNA FFPE kit.
Access protocolTakara Bio USA, Inc.
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