Isolation and purification of total RNA from FFPE samples
The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS kits provide a fast, efficient, and convenient method for isolating both small (e.g., miRNA) and large RNAs from formalin-fixed, paraffin-embedded (FFPE) tissue.
The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS kits provide a fast, efficient, and convenient method for isolating both small (e.g., miRNA) and large RNAs from formalin-fixed, paraffin-embedded (FFPE) tissue.
NucleoSpin totalRNA FFPE includes mini spin columns (30–50 µl elution volume) to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS includes XS columns (5–30 µl elution volume) to purify very small FFPE samples (<5 mg tissue, e.g., microdissections). Both kits include blue-colored Paraffin Dissolver for easy visualization of phase separation and convenient paraffin removal without using toxic organic solvents (such as xylene), and rDNase for efficient on-column DNA removal. The kits combine decrosslinking and tissue lysis in one step, providing reliable yields of high-quality total RNA for enhanced RT-PCR performance. The NucleoSpin totalRNA FFPE XS kit makes it possible to isolate highly concentrated RNA suitable for qRT-PCR from even trace amounts of FFPE samples.
Overview
- Efficient isolation of total RNA from FFPE samples
- Two kit options—high recovery from standard and very small FFPE samples
- Convenient paraffin removal—no xylene required
- Enhanced RT-PCR performance
More Information
| NucleoSpin totalRNA FFPE | NucleoSpin totalRNA FFPE XS | |
| Technology | Silica membrane | Silica membrane |
| Format | Mini spin columns | XS spin columns |
| Starting material | 4–10 sections (10 µm) of up to 6 mm2 total area <50 mg tissue |
1–3 sections (10 µm) of up to 6 mm2 total area <5 mg tissue |
| Typical yield | Depends on the amount and quality of the sample | Depends on the amount and quality of the sample |
| Elution volume | 30–50 µl | 5–30 µl |
| Preparation time | 70 min/6 preps (90 min including optional rDNase digest) | 70 min/6 preps (90 min including optional rDNase digest) |
| Binding capacity | 200 µg | 100 µg |
Applications
- Rapid isolation of RNA from formalin-fixed, paraffin-embedded samples, e.g.:
- Colon carcinoma (unstained or hematoxylin-stained)
- Colon mucosa
- Adenocarcinoma of the colon transversum
- Liver (rat/human)
- Liver carcinoma (hematoxylin-stained)
- Lymph node
- Spleen (rat/human)
- Pancreas (rat/human; diabetic/non-diabetic)
- Placenta
- Isolation of RNA from fresh and archived FFPE samples
- Isolation of RNA from specimens on object slides (stained or unstained)
- Typical downstream application: RT-PCR
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.
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