Efficient plasmid DNA concentration and desalination without a vacuum manifold—NucleoSpin Finisher Midi

Eluates from anion-exchange plasmid preparations (such as NucleoBond Midi preps and Maxi preps) typically contain high salt concentrations. In addition to the vacuum-based NucleoSnap Finisher Midi/Maxi kits, Takara Bio offers NucleoSpin Finisher Midi, which enables rapid concentration and desalination of plasmid DNA using successive centrifugation steps.

Principle/procedure

NucleoSpin Finisher Midi kits supersede the commonly used and time-consuming isopropanol precipitation of DNA from anion exchange eluates by allowing the rapid DNA precipitation onto a filter membrane, followed by a combined washing/drying step and a convenient elution with a variable elution volume. All steps are performed in a centrifuge, which allows the parallel purification of multiple samples.

DNA is precipitated by Buffer FB and filtered by the specially designed matrix of the novel NucleoSpin Finisher Columns, followed by a combined washing and drying step with ethanolic Wash Buffer A4 to remove salts, impurities, and ethanol.

Afterwards, plasmid DNA can be eluted in supplied endotoxin-free H₂O-EF. The applied elution volume can be adjusted from 100 μl to 1.5 ml, according to the expected amount of plasmid DNA, to ensure optimal yield and concentration.

Desalt and concentrate plasmid midipreps and maxipreps.

Application data

Ultra-fast plasmid DNA desalting and concentration.

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Overview

No time-consuming isopropanol precipitation
Used for precipitation of DNA eluates from anion exchange eluates (NucleoBond preparations)

More Information

Technology	Silica-membrane technology
Format	Funnel columns
Compatibility	Eluates from NucleoBond preparations: Xtra Midi (EF), Xtra Maxi (EF), PC 20, PC 100, PC 500 (EF)
Sample material	DNA eluates from anion-exchange purification kits
Typical recovery	90–100%
Elution volume	≥100 µl
Preparation time	15 min/6 preps
Binding capacity	1.5 mg

Applications

Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange chromatography
Typical downstream applications: transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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