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Nucleic acid purification

Efficient plasmid DNA concentration and desalination without a vacuum manifold—NucleoSpin Finisher Midi

Eluates from anion-exchange plasmid preparations (such as NucleoBond Midi preps and Maxi preps) typically contain high salt concentrations. In addition to the vacuum-based NucleoSnap Finisher Midi/Maxi kits, Takara Bio offers NucleoSpin Finisher Midi, which enables rapid concentration and desalination of plasmid DNA using successive centrifugation steps.

Principle/procedure

NucleoSpin Finisher Midi kits supersede the commonly used and time-consuming isopropanol precipitation of DNA from anion exchange eluates by allowing the rapid DNA precipitation onto a filter membrane, followed by a combined washing/drying step and a convenient elution with a variable elution volume. All steps are performed in a centrifuge, which allows the parallel purification of multiple samples.

DNA is precipitated by Buffer FB and filtered by the specially designed matrix of the novel NucleoSpin Finisher Columns, followed by a combined washing and drying step with ethanolic Wash Buffer A4 to remove salts, impurities, and ethanol.

Afterwards, plasmid DNA can be eluted in supplied endotoxin-free H₂O-EF. The applied elution volume can be adjusted from 100 μl to 1.5 ml, according to the expected amount of plasmid DNA, to ensure optimal yield and concentration.

Desalt and concentrate plasmid midipreps and maxipreps.

Application data

Ultra-fast plasmid DNA desalting and concentration.

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740439.50

NucleoSpin® Finisher Midi

50 Preps

USD $258.00

Eluates from anion exchange plasmid preparations (such as NucleoBond Midi preps and Maxi preps) contain high salt concentrations. NucleoSpin Finisher Midi kits supersede the commonly used and time-consuming isopropanol precipitation of DNA from anion exchange eluates by allowing the rapid DNA precipitation onto a filter membrane, followed by a combined washing/drying step and convenient elution with a variable elution volume. All steps are performed in a centrifuge which allows the parallel purification of multiple samples.

Cat. # 740439.50 includes sufficient quantities of reagents and materials to process 50 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. #	Product	Size	Price
740571	NucleoBond® PC 20	20 Preps	USD $164.00
NucleoBond PC 20 (20) 20 preps for the isolation of plasmid DNA - NucleoBond AX 20 Columns, buffers, RNase A Documents You May Also Like Image Data Back 740571: NucleoBond PC 20
740571.100	NucleoBond® PC 20	100 Preps	USD $650.00
NucleoBond PC 20 (100) 100 preps for the isolation of plasmid DNA - NucleoBond AX 20 Columns, buffers, RNase A Documents You May Also Like Image Data Back 740571.100: NucleoBond PC 20 Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.
740410.10	NucleoBond® Xtra Midi	10 Preps	USD $128.00
NucleoBond Xtra Midi (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra Midi/Maxi (midiprep and maxiprep kits) provide up to 60% time savings and up to 100% higher yields compared to competitors’ kits. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740410.50	NucleoBond® Xtra Midi	50 Preps	USD $605.00
NucleoBond Xtra Midi (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Resources Back 740410.50: NucleoBond Xtra Midi Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit). Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.
740410.100	NucleoBond® Xtra Midi	100 Preps	USD $1110.00
Use Midi prep and Midi prep Xtra Plus to purify plasmid DNA in 30 min without the use of vacuum or centrifugation. Typical yield >500 µg of ultra-pure plasmid DNA. Documents Image Data Back Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra Midi/Maxi (midiprep and maxiprep kits) provide up to 60% time savings and up to 100% higher yields compared to competitors’ kits. Back 740410.10: NucleoBond Xtra Midi Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit). Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.
740420.10	NucleoBond® Xtra Midi EF	10 Preps	USD $192.00
NucleoBond Xtra Midi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back 740420.10: NucleoBond Xtra Midi EF Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor.
740420.50	NucleoBond® Xtra Midi EF	50 Preps	USD $875.00
NucleoBond Xtra Midi EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back 740420.50: NucleoBond Xtra Midi EF Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor.
740573	NucleoBond® PC 100	20 Preps	USD $231.00
NucleoBond PC 100 (20) 20 preps for the isolation of plasmid DNA - NucleoBond AX 100 Columns, Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back 740573: NucleoBond PC 100 Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.
740573.100	NucleoBond® PC 100	100 Preps	USD $1050.00
NucleoBond PC 100 (100) 100 preps for the isolation of plasmid DNA - NucleoBond AX 100 Columns, NucleoBond Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back 740573.100: NucleoBond PC 100 Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.

740439.10

NucleoSpin® Finisher Midi

10 Preps

USD $55.00

Cat. # 740439.10 includes sufficient quantities of reagents and materials to process 10 samples.

Notice to purchaser

Required Products

Cat. #	Product	Size	Price
740571	NucleoBond® PC 20	20 Preps	USD $164.00
NucleoBond PC 20 (20) 20 preps for the isolation of plasmid DNA - NucleoBond AX 20 Columns, buffers, RNase A Documents You May Also Like Image Data Back 740571: NucleoBond PC 20
740571.100	NucleoBond® PC 20	100 Preps	USD $650.00
NucleoBond PC 20 (100) 100 preps for the isolation of plasmid DNA - NucleoBond AX 20 Columns, buffers, RNase A Documents You May Also Like Image Data Back 740571.100: NucleoBond PC 20 Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.
740410.10	NucleoBond® Xtra Midi	10 Preps	USD $128.00
NucleoBond Xtra Midi (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra Midi/Maxi (midiprep and maxiprep kits) provide up to 60% time savings and up to 100% higher yields compared to competitors’ kits. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740410.50	NucleoBond® Xtra Midi	50 Preps	USD $605.00
NucleoBond Xtra Midi (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Resources Back 740410.50: NucleoBond Xtra Midi Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit). Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.
740410.100	NucleoBond® Xtra Midi	100 Preps	USD $1110.00
Use Midi prep and Midi prep Xtra Plus to purify plasmid DNA in 30 min without the use of vacuum or centrifugation. Typical yield >500 µg of ultra-pure plasmid DNA. Documents Image Data Back Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra Midi/Maxi (midiprep and maxiprep kits) provide up to 60% time savings and up to 100% higher yields compared to competitors’ kits. Back 740410.10: NucleoBond Xtra Midi Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit). Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.
740420.10	NucleoBond® Xtra Midi EF	10 Preps	USD $192.00
NucleoBond Xtra Midi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back 740420.10: NucleoBond Xtra Midi EF Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor.
740420.50	NucleoBond® Xtra Midi EF	50 Preps	USD $875.00
NucleoBond Xtra Midi EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back 740420.50: NucleoBond Xtra Midi EF Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor.
740573	NucleoBond® PC 100	20 Preps	USD $231.00
NucleoBond PC 100 (20) 20 preps for the isolation of plasmid DNA - NucleoBond AX 100 Columns, Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back 740573: NucleoBond PC 100 Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.
740573.100	NucleoBond® PC 100	100 Preps	USD $1050.00
NucleoBond PC 100 (100) 100 preps for the isolation of plasmid DNA - NucleoBond AX 100 Columns, NucleoBond Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back 740573.100: NucleoBond PC 100 Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.

Overview

No time-consuming isopropanol precipitation
Used for precipitation of DNA eluates from anion exchange eluates (NucleoBond preparations)

More Information

Technology	Silica-membrane technology
Format	Funnel columns
Compatibility	Eluates from NucleoBond preparations: Xtra Midi (EF), Xtra Maxi (EF), PC 20, PC 100, PC 500 (EF)
Sample material	DNA eluates from anion-exchange purification kits
Typical recovery	90–100%
Elution volume	≥100 µl
Preparation time	15 min/6 preps
Binding capacity	1.5 mg

Applications

Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange chromatography
Typical downstream applications: transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Efficient plasmid DNA concentration and desalination without a vacuum manifold—NucleoSpin Finisher Midi

Principle/procedure

Application data

Notice to purchaser

The NucleoSpin Finisher Midi procedure

740439.50: NucleoSpin Finisher Midi

740571: NucleoBond PC 20

740571.100: NucleoBond PC 20

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

740410.50: NucleoBond Xtra Midi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits

740410.10: NucleoBond Xtra Midi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

740420.10: NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

740420.50: NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

740573: NucleoBond PC 100

The NucleoBond PC plasmid purification protocol

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

740573.100: NucleoBond PC 100

The NucleoBond PC plasmid purification protocol

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

Notice to purchaser

740439.10: NucleoSpin Finisher Midi

The NucleoSpin Finisher Midi procedure

740571: NucleoBond PC 20

740571.100: NucleoBond PC 20

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

740410.50: NucleoBond Xtra Midi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits

740410.10: NucleoBond Xtra Midi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

740420.10: NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

740420.50: NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

740573: NucleoBond PC 100

The NucleoBond PC plasmid purification protocol

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

740573.100: NucleoBond PC 100

The NucleoBond PC plasmid purification protocol

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

Overview