NucleoMag RNA Blood
NucleoMag RNA Blood enables magnetic bead-based isolation of high-quality RNA from whole blood samples collected in EDTA/citrate blood collection tubes, SARSTEDT S-Monovette RNA Exact collection tubes, Zymo DNA/RNA Shield Blood Tubes, or Tempus RNA Blood Tubes. Designed for simplicity and efficiency, this kit eliminates the need for reagent removal or pelleting to streamline your workflows. The workflow can be automated on liquid handling platforms in combination with NucleoMag SEP and on magnetic rod systems, such as the IsoPure Mini.
Overview
- Isolate high-quality RNA from blood for downstream applications
- Streamline workflows with a protocol that requires no pelleting or reagent removal
- Conveniently handle samples at room temperature
- Process samples on an automated platform or manually
More Information
| Format | Magnetic beads |
| Sample type | Whole blood |
| Sample amounts | 9 ml of stabilized blood from Tempus Blood RNA Tubes (corresponds to 3 ml whole blood) 260 µl blood in EDTA/Citrate blood collection tubes 820 µl of stabilized blood from SARSTEDT S-Monovette RNA Exact tubes (corresponds to 200 µl whole blood) 820 µl stabilized blood from DNA/RNA Shield Blood Collection Tubes (corresponds to 270 µl whole blood) |
| Fragment size | >200 nt |
| Binding capacity | 0.4 µg/1 µl B-beads |
| Typical yield | 3–4 µg |
| Automated platform compatibility | Compatible with most open robotic platforms. Please contact technical support for more information. |
| Elution volume | 50–100 µl |
| Preparation time | 90–120 min, depending on manual or automated processing |
Applications
- RNA isolation from blood for downstream applications, including cDNA library construction, microarray spotting, NGS, and RT-qPCR
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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