RNA isolation or purification—mini spin columns
NucleoSpin RNA is recommended for isolation and purification of total RNA from cultured cells, tissue, bacteria, yeast, cell-free biological fluids, and reaction mixtures without the use of organic compounds (e.g., Trizol). This kit allows purification of up to 70 µg of highly pure, DNA-free total RNA. Alternatively, you may be interested to learn about the next generation of this RNA purification kit, NucleoSpin RNA Plus.
NucleoSpin RNA is recommended for isolation and purification of total RNA from cultured cells, tissue, bacteria, yeast, cell-free biological fluids, and reaction mixtures without the use of organic compounds (e.g., Trizol). This kit allows purification of up to 70 µg of highly pure, DNA-free total RNA. Alternatively, you may be interested to learn about the next generation of this RNA purification kit, NucleoSpin RNA Plus.
Total RNA prepared with NucleoSpin RNA is suitable for applications such as real-time reverse transcription PCR (qRT-PCR), Northern blotting, primer extension, array technology, and RNase protection assays. For isolation of high-quality RNA, it is important to prevent degradation of the RNA and to eliminate genomic DNA. With the NucleoSpin RNA method, cells are lysed in the presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. After lysis, homogenization and reduction of viscosity are achieved by filtration with NucleoSpin Filter units (shredders), which are provided with the kit. Residual genomic DNA is efficiently removed by on-column digestion with rDNase, which is also included in the kit.
Overview
- Complete purification kit: DNase I and shredders are included
- No genomic DNA contamination
- Yields up to 70 µg of high-quality, ready-to-use RNA without the use of organic compounds (e.g., Trizol)
- Simple spin column protocol (no need to learn a new protocol)
- Better performance and outstanding savings
- Columns and buffers are available separately
- Cat. # 740955.240C is designed for use with the QIAcube only
More Information
| Technology | Silica membrane |
| Format | Mini spin columns |
| Starting material | <5 x 106 cultured cells <109 bacterial cells <30 mg tissue up to 108 yeast cells |
| Fragment size | >200 bp |
| Typical yield | 14–70 µg |
| Elution volume | 40–100 µl |
| Preparation time | 30 min/6 preps |
Applications
Kit for isolation and purification of total RNA from cultured cells and tissue (standard protocol), as well as paraffin-embedded tissue, bacterial cells, yeast cells, biological fluids, and samples stored in RNAlater (support protocols) without the use of organic compounds (e.g., Trizol).
Obtain purified RNA suitable for:
- Real-time RT-PCR
- Northern blotting
- Primer extension
- Array technology
- RNase protection assays
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.
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