miRNA isolation or extraction from plasma and serum—NucleoSpin miRNA Plasma
The NucleoSpin miRNA Plasma procedure is a simple and fast method to isolate small RNA (>15 nt), such as microRNA (miRNA), from serum or plasma. The DNA can be isolated as well or digested on-column to eliminate interference of miRNA genes with downstream applications.
The NucleoSpin miRNA Plasma procedure is a simple and fast method to isolate small RNA (>15 nt), such as microRNA (miRNA), from serum or plasma. The DNA can be isolated as well or digested on-column to eliminate interference of miRNA genes with downstream applications.
This proven, efficient silica membrane-based procedure isolates total RNA and DNA from plasma without the need to resort to cumbersome phenol/chloroform extraction or to a time-consuming proteinase digestion. The sample material is denatured in Lysis Buffer MLP. The protein is then precipitated by Protein Precipitation Buffer MPP and pelleted by centrifugation. After the removal of protein, the binding conditions for nucleic acids are adjusted by adding isopropanol, allowing small RNA and DNA to bind to the NucleoSpin miRNA Column. Optionally, DNA can be removed by an on-column rDNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-free water.
Overview
- Efficient isolation of small RNA from plasma or serum samples
- Superior recovery of small RNA (>15 nt)
- Optional on-column DNA digest for increased sensitivity in downstream applications
More Information
| Format | Mini spin columns |
| Sample material | 300 µl plasma or serum (up to 900 µl with multiple loading) |
| Fragment size | <1,000 nt/bp |
| Elution volume | 20–50 µl |
| Preparation time | 40 min/10 preps (without rDNase digestion) 70 min/10 preps (with rDNase digestion) |
| Binding capacity | 200 µg |
| Technology | Silica membrane |
Applications
- Isolation of small RNAs from plasma or serum for cancer research
- Typical downstream applications: qRT-PCR, chip hybridization
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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