Plasmid DNA purification kit selection guide

Our plasmid DNA purification kits help you obtain reliable, high-quality plasmid DNA in minutes. We provide a wide range of formats from mini- to gigapreps as well as options for high-throughput processing. Specialized kits are also available for purifying plasmid DNA suitable for various downstream applications, such as transfection, sequencing, etc. Use the tables below to learn more about the available kits and their specifications.

Kit specifications

Quality grade	Application	Scale	Typical yield	Kit name
Molecular biology (>>50 EU/µg DNA)	Sequencing, cloning	Mini	25–45 µg	NucleoSpin Plasmid
			25–45 µg	NucleoSpin Plasmid (No Lid)
			15–30 µg	NucleoSpin Plasmid EasyPure
		8 well	5–20 µg	NucleoSpin 8 Plasmid
		96 well	5–20 µg	NucleoSpin 96 Plasmid
		96 well	<8 µg	NucleoSpin 96 Flash
Molecular biology (<50 EU/µg DNA)	Transfection (nonsensitive cells)	Mini	3–20 µg	NucleoBond PC 20
		Mini	15–30 µg	NucleoSpin Plasmid Transfection-grade
		96 well	5–20 µg	NucleoSpin 96 Plasmid Transfection-grade
		Midi	500 µg	NucleoBond Xtra Midi
			20–100 µg	NucleoBond PC 100
			250 µg	NucleoSnap Plasmid Midi
		Maxi	1,000 µg	NucleoBond Xtra Maxi
			400–500 µg	NucleoBond PC 500
			10–150 µg	NucleoBond Xtra BAC*
			10–100 µg	NucleoBond BAC 100*
		Mega	0.5–2 mg	NucleoBond PC 2000
		Giga	2–10 mg	NucleoBond PC 10000
Endotoxin-free (<0.1 EU/µg DNA)	Transfection (sensitive cells)	96 well	<50 µg	NucleoBond Xtra 96 EF
		Midi	500 µg	NucleoBond Xtra Midi EF
		Maxi	1,000 µg	NucleoBond Xtra Maxi EF
		Maxi	100–500 µg	NucleoBond PC 500 EF
		Mega	0.5–2 mg	NucleoBond PC 2000 EF
		Giga	2–10 mg	NucleoBond PC 10000 EF
		Preparative	80–100 mg	NucleoBond Prep 100
Vaccine-grade (<0.01 EU/µg DNA)	AAV production, cell and gene therapy development	Preparative	10 mg	NucleoBond RS 10
			50 mg	NucleoBond RS 50
			100 mg	NucleoBond RS 100
			200 mg	NucleoBond RS 200

Ultra-fast procedure; EU: Endotoxin unit, *Special kit for large constructs

Kit formats

	NucleoSpin	NucleoSpin 8	NucleoSpin 96	NucleoSnap	NucleoBond	NucleoBond 96
Technology	Silica membrane	Silica membrane	Silica membrane	Silica membrane	Anion exchange	Anion exchange
Format	Mini spin column	8-well strip	96-well plate	Midi snap-off column	Mini–giga columns	96-well plate
Processing	Centrifugation or vacuum	Vacuum or centrifugation	Vacuum or centrifugation	Vacuum (centrifugation for elution)	Gravity flow	Vacuum or centrifugation

Tip for a successful transfection—remove endotoxins!

Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.

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