A fast way to purify plasmids for transfection—NucleoSpin Plasmid Transfection-grade
NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses an innovative technology developed by Macherey-Nagel to reduce the level of endotoxins copurified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Click here to see performance data. A 96-well format is also available.
Overview
- Novel technology to diminish endotoxin content (<50 EU/µg DNA) for successful transfection
- A miniprep purification format simplifies your workflow
- Ultra-fast procedure
More Information
| Technology | Silica membrane and endotoxin-reduction technologies |
| Format | Miniprep spin columns |
| Endotoxin content | <50 EU/µg DNA (EU=Endotoxin Units) |
| Starting material | Up to 5 ml bacterial culture (E. coli, high-copy plasmids) |
| Vector size | <15 kb |
| Typical yield | 5–20 µg |
| A260/A280 | 1.80–1.85 |
| Elution volume | 30–50 µl |
| Preparation time | 14 min/6 preps |
| Binding capacity | 25 µg |
Applications
- Typical downstream applications: transfection of common cell lines (lipofection, electroporation)
- Also suitable for standard molecular biology applications such as cloning and sequencing
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Tip for a successful transfection—remove endotoxins!
Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.
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